Microfiltración coronal según materiales de restauración temporal empleados en endodoncia / Coronal microleakage according to the temporary restorative materials used in endodontic treatment

RESUMEN Introducción: La microfiltración coronal causada por los materiales de restauración temporal es considerada una de las causas del fracaso de los tratamientos endodónticos. A raíz de ello, en los últimos años se ha buscado crear un material de restauración temporal que sea capaz de evitar este problema. Objetivo: Comparar in vitro la microfiltración coronal de un cemento experimental y cuatro materiales de restauración temporal usados en endodoncia. Métodos: Se realizaron cavidades clase I en 90 premolares, divididos en 10 grupos (n= 9) y evaluados en dos periodos de tiempo (1 y 2 semanas). Se realizó la elaboración del cemento experimental, posteriormente se llevó a cabo el sellado coronal de las piezas dentales con el cemento experimental, Clip F (VOCO), Eugenato (MOYCO), Ketac™ Molar Easymix 3M (ESPE) y Coltosol® F. Los especímenes fueron sumergidos en tinta china (Pelikan) durante 1 y 2 semanas. Se evaluó la microfiltración en la interfase pared dentinaria-restauración temporal utilizando un esteromicroscopio (Leica Microsystems LAS EZ versión 2.0.0). La medición se realizó en milímetros en el programa LAS EZ versión 2.0.0. Resultados: Se encontró diferencias estadísticamente significativas (p < 0,05) al comparar la microfiltración coronal de los cinco materiales de restauración temporal, según el tiempo de exposición en tinta (1 y 2 semanas). Conclusiones: El cemento experimental presentó menor microfiltración que el Coltosol® F y Ketac™ Molar EasyMix 3M (ESPE); sin embargo, ninguno de los cuatro materiales fue capaz de prevenir la microfiltración en su totalidad(AU)

ABSTRACT Introduction: Coronal microleakage caused by temporary restorative materials is viewed as one of the reasons for endodontic failure. Efforts have been made in recent years to create a temporary restorative material capable of preventing that problem. Objective: Compare in vitro coronal microleakage of an "experimental cement" and four temporary restorative materials used in endodontics. Methods: Class I cavities were made in 90 premolar teeth divided into 10 groups (n= 9) and evaluated at two time periods (1 and 2 weeks). The "experimental cement" was prepared and then coronal sealing of the teeth was performed with the experimental Cement, F (VOCO), Eugenato (MOYCO), Ketac™ Molar Easymix 3M (ESPE) and Coltosol® F. The pieces were submerged in India ink (Pelikan)for 1 and 2 weeks. Microleakage was evaluated on the interface dentin wall / temporary restoration using a stereo microscope (Leica Microsystems LAS EZ version 2.0.0). Measurement was made in millimeters using the software LAS EZ version 2.0.0. Results: Statistically significant differences were found (p < 0.05) when comparing coronal microleakage in the five temporary restorative materials according to exposure time in ink (1 and 2 weeks). Conclusions: The experimental cement had less microleakage than Coltosol® F and Ketac™ Molar EasyMix 3M (ESPE). However, none of the four materials was capable of completely preventing microleakage(AU)

Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis.

OBJECTIVE: To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. METHODS: The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. RESULTS: The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. CONCLUSIONS: In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.

Capacidad de permeabilización en retratamiento de conductos subobturados mediante tres cementos selladores / Permeabilization capacity in root canal retreatment underfilling using three sealers

Introducción: En el presente estudio in vitro se evaluó la permeabilidad de los conductos subobturados con tres cementos selladores, los cuales fueron retratados de manera químico - mecánica con el objetivo de alcanzar la longitud de trabajo óptima. Material y método: Fueron utilizados 60 conductos de las raíces mesiales de molares inferiores permanentes, divididos en tres grupos: (G1) Gutapercha - Endofill, (G2) Gutapercha - Roeko seal y (G3) el sistema de obturación Resilon (conos de resina y Epiphany Seal); los cuales fueron retratados mediante el sistema Pro-taper Re tratamiento (D1, D2) y el cloroformo como disolvente. Resultados: Fueron evaluados cualitativamente empleando la prueba de chi cuadrado, encontrándose que fue estadísticamente significativa (p<0,005) entre los grupos (G1) gutapercha-endofill y (G2) gutapercha Roeko seal en comparación con el (G3) sistema Resilon Epiphany seal. Conclusiones: El estudio demostró que los cementos selladores Endofill y Roeko Seal presentaron mayor factibilidad en la permeabilización que el Epiphany en conductos subobturados.

Introduction: In this in vitro study, there was evaluated the permeability of the underfilling root canals with three sealers, which were portrayed in chemical-mechanical way in order to achieve optimal working length. Material and Methods: There were used 60 mesial root canals of permanent lower molars divided (G1) Gutta-percha - Endofill, (G2) Gutta-percha - Roek Seal and (G3) Resilon obturation system (cones and resin Epiphany Seal), which were portrayed by the Pro-taper Re treatment (D1, D2) and chloroform as solvent. Results: There were evaluated qualitatively using the Chi Square test finding that it was statistically significant (p <0.005) among the groups (G1) and gutta-endofill (G2) Roek percha seal compared to the (G3) Epiphany Resilon system seal. Conclusions: The study showed that sealers: Endofill and Roeko seal showed higher permeability than the Epiphany in under filling root canals.