Survey data on Families' perceptions of ed-tech corporations, educational digital platforms and children's rights.

This data article describes the dataset of the project "edDIT: Technological corporations, digital educational platforms and guarantee of children's rights with a gender approach". This study has analysed the impact of the use of corporate digital platforms in public schools in Catalonia. A series of data were collected through an online survey, with a total sample of 2347 parents/caregivers. The description of the data contained in this article is divided into two main parts. The first one is a descriptive analysis of all the items included in the survey and has been carried out using tables and figures. The second one refers to the construction of scales. Three scales were constructed and included in the data set: 'Opinions about Educational Digital Platforms', 'Concerns about the use of the data generated on the utilisation of the digital platform' and 'Parental Engagement'. The scales were created using Confirmatory Factor Analysis (CFA) and Multigroup Confirmatory Analysis (MG-CFA). This dataset will be relevant for researchers in different fields, in particular for those interested in digital inclusion public policies and educational policies.

Angiocrine polyamine production regulates adiposity.

Reciprocal interactions between endothelial cells (ECs) and adipocytes are fundamental to maintain white adipose tissue (WAT) homeostasis, as illustrated by the activation of angiogenesis upon WAT expansion, a process that is impaired in obesity. However, the molecular mechanisms underlying the crosstalk between ECs and adipocytes remain poorly understood. Here, we show that local production of polyamines in ECs stimulates adipocyte lipolysis and regulates WAT homeostasis in mice. We promote enhanced cell-autonomous angiogenesis by deleting Pten in the murine endothelium. Endothelial Pten loss leads to a WAT-selective phenotype, characterized by reduced body weight and adiposity in pathophysiological conditions. This phenotype stems from enhanced fatty acid ß-oxidation in ECs concomitant with a paracrine lipolytic action on adipocytes, accounting for reduced adiposity. Combined analysis of murine models, isolated ECs and human specimens reveals that WAT lipolysis is mediated by mTORC1-dependent production of polyamines by ECs. Our results indicate that angiocrine metabolic signals are important for WAT homeostasis and organismal metabolism.

Identification of Androgen Receptor Metabolic Correlome Reveals the Repression of Ceramide Kinase by Androgens.

Prostate cancer (PCa) is one of the most prevalent cancers in men. Androgen receptor signaling plays a major role in this disease, and androgen deprivation therapy is a common therapeutic strategy in recurrent disease. Sphingolipid metabolism plays a central role in cell death, survival, and therapy resistance in cancer. Ceramide kinase (CERK) catalyzes the phosphorylation of ceramide to ceramide 1-phosphate, which regulates various cellular functions including cell growth and migration. Here we show that activated androgen receptor (AR) is a repressor of CERK expression. We undertook a bioinformatics strategy using PCa transcriptomics datasets to ascertain the metabolic alterations associated with AR activity. CERK was among the most prominent negatively correlated genes in our analysis. Interestingly, we demonstrated through various experimental approaches that activated AR reduces the mRNA expression of CERK: (i) expression of CERK is predominant in cell lines with low or negative AR activity; (ii) AR agonist and antagonist repress and induce CERK mRNA expression, respectively; (iii) orchiectomy in wildtype mice or mice with PCa (harboring prostate-specific Pten deletion) results in elevated Cerk mRNA levels in prostate tissue. Mechanistically, we found that AR represses CERK through interaction with its regulatory elements and that the transcriptional repressor EZH2 contributes to this process. In summary, we identify a repressive mode of AR that influences the expression of CERK in PCa.

Genetic lesions in MYC and STAT3 drive oncogenic transcription factor overexpression in plasmablastic lymphoma.

Plasmablastic lymphoma mutational profile is undescribed. Here we performed a targeted exonic NGS analysis of 30 plasmablastic lymphoma cases with a B cell lymphoma dedicated panel and FISH for the detection of MYC rearrangements. A complete phenotyping of the neoplastic and microenvironment cell populations was also performed. We have identified an enrichment in recurrent genetic events in MYC (69% with MYC translocation or amplification and 3 cases with missense point mutations), PRDM1/Blimp1 and STAT3 mutations. These gene mutations were more frequent in EBV positive disease. Other genetic events included mutations in BRAF, EP300, BCR (CD79A and CD79B), NOTCH pathway (NOTCH2, NOTCH1 and SGK1) and MYD88pL265P. Immunohistochemical analysis showed consistent MYC expression, higher in cases with MYC rearrangements together with phospho-STAT3 (Tyr705) overexpression in cases with STAT3 SH2 domain mutations. Microenvironment populations were heterogeneous and unrelated with EBV, with an enrichment of Tumor Associated Macrophages (TAM) and PD1 positive T cells. PD-L1 was expressed in all cases in the TAM population but only in 5 cases in the neoplastic cells (4 out of 14 EBV positive cases). HLA expression was absent in the majority of PBL cases. In summary, Plasmablastic lymphoma mutational profile is heterogeneous and related with EBV infection. Genetic events in MYC, STAT3 and PRDM1/Blimp1 are more frequent in EBV positive disease. An enrichment in TAM and PD1 reactive T lymphocytes is found in the microenvironment of PBL cases, that express PD-L1 in the neoplastic cells in a fraction of cases.

Diagnostic value of bone marrow core biopsy patterns in lymphoplasmacytic lymphoma/Waldenström macroglobulinaemia and description of its mutational profiles by targeted NGS.

AIMS: The aim of this study was to describe the characteristics of the bone marrow infiltration found in a series of clinically defined lymphoplasmacytic lymphoma (LPL)/Waldenström macroglobulinaemia (WM) and IgM-monoclonal gammopathy of undetermined significance (MGUS) and to perform a targeted next-generation sequencing (NGS) for the identification of additional somatic mutations to MYD88p.L265P in LPL/WM. METHODS: We have reviewed a series of 35 bone marrow biopsies from 28 patients with a clinical diagnosis of LPL/WM (24 cases) or MGUS (4 cases). Bone marrow infiltration characteristics by morphology, immunohistochemistry, flow cytometry (FCM), allele-specific real-time PCR for the detection of MYD88p.L265P mutation, targeted exonic amplicon-based NGS of 35 lymphoma-related genes and direct sequencing were analysed. RESULTS: Our findings show that bone marrow trephine biopsy evaluation is superior to FCM in the identification of significant lymphoid infiltrates. A combined paratrabecular and interstitial infiltration pattern is the most common feature in LPL/WM while a patchy interstitial pattern characterises IgM-MGUS cases. MYD88p.L265P mutation was found by allele-specific-PCR in 92% of the LPL cases (22 out of 24) and 25% of IgM-MGUS cases (1 out of 4 cases). In addition to MYD88p.L265P somatic mutations in CXCR4, KMT2D, PRDM1/Blimp1, MYC and ID3 were found by NGS and direct sequencing in 4 cases. CONCLUSIONS: In conclusion, bone marrow core biopsy evaluation is critical in the identification of unequivocal bone marrow infiltration by LPL/WM. In addition to MYD88p.L265P, somatic mutations in CXCR4, KMT2D, PRDM1/Blimp1, MYC and ID3 can appear in a fraction of LPL/WM.

Salud física de la persona con trastorno mental grave: sensibilización a los profesionales de atención primaria / No disponible

El presente trabajo se propone recoger la opinión y actitudes de médicos, enfermeros y trabajadores sociales de los Centros de Atención Primaria (CAP) de Ibiza y Pacífico, pertenecientes al distrito de Retiro, sobre la atención que llevan a cabo en sus consultas a los pacientes con Trastorno Mental Grave (TMG), así como sensibilizar acerca de la problemática de la salud física y el estigma asociados. Esta acción se materializó a través de la aplicación de una encuesta anónima con formato de preguntas cerradas y abiertas que recoge la percepción general ante el paciente con TMG por parte del profesional de Atención Primaria (AP), la autopercepción de conocimientos o formación en materia de enfermedad mental y los problemas de salud física más comúnmente asociados, los recursos del entorno promotores de hábitos saludables y la coordinación existente entre dispositivos y niveles asistenciales. Tras el análisis descriptivo de los resultados, se pone de manifiesto: Que los profesionales de atención primaria tienen mucho que decir respecto a los pacientes con TMG a los que atienden. Que presentan actitudes de rechazo, miedo y estigma relacionadas con dificultades en el manejo por la falta de conocimientos y formación en salud mental así como de la comorbilidad física que presentan. Y que es necesario mejorar la coordinación entre dispositivos. Estos resultados fueron devueltos a los profesionales en una acción de sensibilización llevado a cabo en los CAP acerca del estado de salud física del paciente con TMG, de donde surgieron propuestas de coordinación y colaboración entre dispositivos para mejorar la atención del usuario y el cuidado de su salud física

The present work aims to collect the opinion and attitudes of doctors, nurses and social workers of the Primary Care Centers (PCC) of Ibiza and Pacific, belonging to the district of Retiro, about the care they carry out in their consultations to patients with Severe Mental Disorder (SMD), as well as raising awareness about the associated physical health problems. This action was materialized through the application of an anonymous survey in the form of closed and open questions that reflects the general perception of the patient with SMD by the Primary Care Professional (PCC), the self-perception of knowledge or training in the field of mental illness and the physical health problems most commonly associated, the resources of the environment promoting healthy habits and the existing coordination between devices and levels of care. After the descriptive analysis of the results, it becomes clear: That the primary care professionals have a lot to say about the patients with SMD that they attend. They present attitudes of rejection, fear and stigma related to difficulties in handling due to the lack of knowledge and training in mental health as well as the physical comorbidity they present. And also that it is necessary to improve the coordination between devices. These results were returned to the professionals in an awareness-raising action carried out in the CAPs about the physical health status of the patient with SMD, from which proposals for coordination and collaboration between devices emerged to improve the user's attention and care of their patients

Only spontaneous counterfactual thinking is impaired in patients with prefrontal cortex lesions.

Counterfactual thoughts (CFT) are mental simulations of what might have been if another behavior had been executed. They are pervasive in everyday life, help people learn from experience, modulate their emotional state, and contribute to decision-making and social functioning. To test the hypothesis that the prefrontal cortex (PFC) is involved in the generation, content, and use of CFT, we studied 18 patients with strictly prefrontal cortex lesions. Our results indicated that the PFC is crucial only for self-generated counterfactual reflections. We did not detect CFT generation differences based on lesion location within the PFC. CFT performance correlated positively with measures of attention, creativity, verbal skills, conscientiousness, and self-esteem and negatively with depression and dysexecutive symptoms. An impairment in counterfactual thinking may contribute to the lack of regret and insight often observed in patients with frontal lobe lesions.