Assuntos
Fosfatase Alcalina/metabolismo , Doenças dos Cavalos/enzimologia , Íleo/irrigação sanguínea , Obstrução Intestinal/veterinária , Isquemia/veterinária , Isoenzimas/metabolismo , Fosfatase Alcalina/sangue , Animais , Líquido Ascítico/enzimologia , Cavalos , Obstrução Intestinal/enzimologia , Isquemia/enzimologia , Isoenzimas/sangueRESUMO
Thyroid microsomes for use in assay systems for anti-thyroid-microsomal antibodies are normally prepared by ultracentrifugation. By means of gel filtration studies we show that preparations made in this way are invariably contaminated with thyroglobulin. A further purification by gel filtration is described which enables the preparation of thyroid microsomes of sufficient purity for use in a micro-ELISA method for the measurement of anti-thyroid-microsomal antibodies. Microsomes prepared in this way would also be suitable for haemagglutination and radioassays, which are also affected by thyroglobulin contamination.
Assuntos
Autoanticorpos/análise , Microssomos/imunologia , Glândula Tireoide/imunologia , Antígenos/isolamento & purificação , Ensaio de Imunoadsorção Enzimática/métodos , Testes de Hemaglutinação , Humanos , Técnicas In Vitro , Radioimunoensaio/métodos , Tireoglobulina/isolamento & purificação , UltracentrifugaçãoRESUMO
An indirect, enzyme-linked immunosorbent assay is described for the assay of thyroid autoantibodies, particularly those directed against thyroglobulin. The method is specific, sensitive and precise, and may be automated. The results are shown to correlate well with those obtained by the haemagglutination method.
