RESUMO
Rats were divided into the following four groups namely (a) sham-operated control, (b) 6-OHDA-treated, (c) sham-operated vitamin E-fed and (d) Vitamin E-fed treated with 6-OHDA. Total glutathione (GSH) and superoxide dismutase (SOD) were measured in brainstem (BS), striatum (ST), hippocampus, frontal cortex, and nucleus accumbens (N. Acc.). GSH and SOD levels were significantly decreased in all regions of 6-OHDA-treated rats compared to controls. Feeding of vitamin E resulted in a significant reduction of GSH in ST and N. Acc. but caused increases in SOD in BS, ST, and N. Acc. Pretreatment of rats with vitamin E caused significant attenuation of the effects of 6-OHDA on GSH and SOD in most of the brain regions. These results show that vitamin E can spare the scavenging systems from the injurious effects of 6-OHDA.
Assuntos
Sequestradores de Radicais Livres , Oxidopamina/antagonistas & inibidores , Vitamina E/farmacologia , Animais , Encéfalo/anatomia & histologia , Química Encefálica , Glutationa/metabolismo , Masculino , Oxidopamina/toxicidade , Ratos , Ratos Sprague-Dawley , Superóxido Dismutase/metabolismoRESUMO
Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were measured in different regions of the brains of control and IDPN-treated rats. IDPN treatment resulted in significant increase of SOD activity in the cerebellum. Other regions, except frontal cortex, exhibited a slight decrease. While there was no significant difference in GSH-Px activity in all regions compared to controls, CAT activity was slightly increased in the cerebellum. These results give credence to the notion that free radical damage to certain areas of the brain such as isodendric core of the brainstem may play a role in the development of movement disorders.
