Comparative study on the immunogenic properties of Clostridium perfringens type A toxoid.

The paper presents the results of a study on the immunogenic properties of toxoid preparations from Cl. perfringens type A obtained using the routine method of detoxifying alpha = toxin in the culture medium (commercial preparations) and by means of detoxifying a previously purified alpha = toxin (experimental preparations). When tested in immunized guinea pigs, the immunogenicity of experimental preparations was found to be 4.5 to 6 times that of commercial preparations. In mice, there was no difference in the immunogenic properties of the two types of preparations as determined by the ED30 of the antigen and the serum levels of Cl. perfringens antitoxin. The possibility is discussed of using the guinea pig as a laboratory animal model due to its ability to reflect most clearly the differences in the immunogenicity of Cl. perfringens type A toxoid preparations.

[Effect on helper activity of synthetic polyelectrolytes introduced into an in vitro system of interacting macrophages and thymus cells]. / Vliianie na khelpernuiu aktivnost' sinteticheskikh poliélektrolitov, vvedennykh v sistemu in vitro vzaimodeistvuiushchikh makrofagov i kletok timusa.

Our experiments have shown that the joint incubation of thymocytes and syngeneic macrophages in vitro for 60 minutes is accompanied by a certain increase in the helper activity of thymocytes. The presence of polyacrylic acid or the copolymer of acrylic acid and N-vinyl-pyrrolidone, containing 56 mol% of acrylic acid, in this system ensures a considerable increase in the helper activity of thymocytes, incubated jointly with marrow cells and sheep red blood cells and then transferred into a cell culture in vivo.

[Mechanism of immunosuppression in carcinogenesis. I. Contact interaction of macrophages with T-lymphocytes in the dynamics of tumor growth]. / Mekhanizm immunosupressii pri kantserogeneze. Soobshchenie I. Kontaktnoe vzaimodeistvie makrofagov s T-limfotsitami v dinamike opukholevogo rosta.

The suppression of the "grape-forming" activity of macrophages has been shown to occur in the process of the development of tumor growth under the conditions of in vitro culture, which seems to be one of the important factors ensuring the "deficit" state in the population of mature lymphocytes.