RESUMO
BACKGROUND: In the male Drosophila, the X chromosome is transcriptionally upregulated to achieve dosage compensation, in a process that depends on association of the MSL proteins with the X chromosome. A role for non-coding RNAs has been suggested in recent studies. The roX1 and roX2 RNAs are male-specific, non-coding RNAs that are produced by, and also found associated with, the dosage-compensated male X chromosome. Whether roX RNAs are physically part of the MSL complex has not been resolved. RESULTS: We found that roX RNAs colocalize with the MSL proteins and are highly unstable unless the MSL complex is coexpressed, suggesting a physical interaction. We were able to immunoprecipitate roX2 RNA from male tissue-culture cells with antibodies to the proteins Msl1 and Mle, consistent with an integral association with MSL complexes. Localization of roX1 and roX2 RNAs in mutants indicated an order of MSL-complex assembly in which roX2 RNA is incorporated early in a process requiring the Mle helicase. We also found that the roX2 gene, like roX1, is a nucleation site for MSL complex spreading into flanking chromatin in cis. CONCLUSIONS: Our results support a model in which MSL proteins assemble at specific chromatin entry sites (including the roX1 and roX2 genes); the roX RNAs join the complex at their sites of synthesis; and complete complexes spread in cis to dosage compensate most genes on the X chromosome.
Assuntos
Mecanismo Genético de Compensação de Dose , Proteínas Nucleares/metabolismo , RNA/metabolismo , Cromossomo X/genética , Animais , Cromatina/genética , Cromatina/metabolismo , Drosophila/genética , Drosophila/metabolismo , Genes de Insetos , Hibridização in Situ Fluorescente , Masculino , Proteínas Nucleares/genética , Testes de Precipitina , RNA/genética , Transgenes , Cromossomo X/metabolismoRESUMO
The multisubunit MSL dosage compensation complex binds to hundreds of sites along the Drosophila single male X chromosome, mediating its hypertranscription. The male X chromosome is also coated with noncoding roX RNAs. When either msl3, mle, or mof is mutant, a partial MSL complex is bound at only approximately 35 unusual sites distributed along the X. We show that two of these sites are the roX1 and roX2 genes and postulate that one of their functions is to provide entry sites for the MSL complex to recognize the X chromosome. The roX1 gene provides a nucleation site for extensive spreading of the MSL complex into flanking chromatin even when moved to an autosome. The spreading can occur in cis or in trans between paired homologs. We present a model for how the dosage compensation complex recognizes X chromatin.
Assuntos
Cromatina/genética , DNA/metabolismo , Mecanismo Genético de Compensação de Dose , Proteínas de Drosophila , Drosophila melanogaster/genética , Genes de Insetos , Proteínas de Insetos/metabolismo , Proteínas Nucleares/metabolismo , Proteínas de Ligação a RNA/genética , RNA/metabolismo , Fatores de Transcrição/metabolismo , Cromossomo X/genética , Animais , Cromatina/metabolismo , Proteínas de Ligação a DNA , Drosophila/genética , Evolução Molecular , Regulação da Expressão Gênica , Masculino , Modelos Genéticos , Ligação Proteica , Especificidade da Espécie , Transcrição Gênica , Transgenes , Cromossomo X/metabolismoRESUMO
The cerebellum is essential for fine motor control of movement and posture, and its dysfunction disrupts balance and impairs control of speech, limb and eye movements. The developing cerebellum consists mainly of three types of neuronal cells: granule cells in the external germinal layer, Purkinje cells, and neurons of the deep nuclei. The molecular mechanisms that underlie the specific determination and the differentiation of each of these neuronal subtypes are unknown. Math1, the mouse homologue of the Drosophila gene atonal, encodes a basic helix-loop-helix transcription factor that is specifically expressed in the precursors of the external germinal layer and their derivatives. Here we report that mice lacking Math1 fail to form granule cells and are born with a cerebellum that is devoid of an external germinal layer. To our knowledge, Math1 is the first gene to be shown to be required in vivo for the genesis of granule cells, and hence the predominant neuronal population in the cerebellum.
