RESUMO
Transfer of conjugative hybrid plasmid RP4::Mu cts 62 from Escherichia coli into Bac. cereus, Bac. thuringiensis, Bac. mesentericus and Bac. polymyxa cells led to the multiple effects on the structure and physiology of bacillus cells. It has resulted in a decrease of the bacillus vitality, in the accelerated autolytic decay of cells, in the delay of cell growth and reproduction rate in liquid and solid media, in the disruption of ultrastructure of the cell membrane and its surface layer.
Assuntos
Bacillus/genética , Plasmídeos/genética , Transformação Bacteriana/genética , Bacillus/ultraestrutura , Conjugação Genética , Resistência Microbiana a Medicamentos/genética , Escherichia coli/genética , Microscopia EletrônicaRESUMO
Electron-microscopic and ultrahistochemical investigations of biopsies of the conjunctiva as well as of iris and retina of 46 patients with diabetes mellitus have revealed disturbances in the ultrastructure, bioenergetics and activity of enzyme processes of the cell at different stages of diabetic angioretinopathy. The increase in activity of peroxide oxidation of lipids in progression of retinopathy is recorded. It is recommended to use antioxidants and agents increasing oxidation-reduction processes.
Assuntos
Retinopatia Diabética/enzimologia , Metabolismo Energético , Adolescente , Adulto , Biópsia , Doença Crônica , Túnica Conjuntiva/enzimologia , Túnica Conjuntiva/ultraestrutura , Feminino , Histocitoquímica , Humanos , Iris/enzimologia , Iris/ultraestrutura , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Retina/enzimologia , Retina/ultraestruturaRESUMO
The localization of Na+,K+-ATPase in cardiomyocytes of white rats at the ultrastructural level and the dependence of enzyme activeness on electronic density of structures, were studied. Na+,K+-ATPase activity was revealed in plasmalemma, Z-membranes and in nuclei. The enzyme activity is distributed discretely both within the tissue and within separate cells. It is, probably, due to the heterogeneity of structural-functional condition of cells and their ultrastructures as revealed by means of electronic microscopy in terms of their different electronic density. The maximal Na+,K+-ATPase activeness was revealed at the average electronic density of structures.
