In vitro investigation of wound healing performance of PVA/chitosan/silk electrospun mat loaded with deferoxamine and ciprofloxacin.

Electrospinning is an advanced method used for developing wound dressings. Biopolymer-based electrospun mats have been extensively studied in tissue engineering due to their similarity to the extracellular matrix. In this study, electrospun poly(vinyl alcohol)/chitosan/silk fibroin (PChS) mat demonstrated improved mechanical properties, including tensile strength, strain at break, and Young's modulus, compared to electrospun poly(vinyl alcohol) and poly(vinyl alcohol)/chitosan mats. Similarly, the swelling capability, thermal stability, and hydrophilicity were higher in the PChS mat compared to the other ones. Hence, the PChS mat was selected for further investigation. Ciprofloxacin (CIP) was added to the PChS electrospinning solution at 5 % and 10 % concentration, and deferoxamine (DFO) was immobilized on CIP-loaded mats at 1 and 2 g/L concentration using a polydopamine linker. Evaluating mats with the dimensions of 1 × 1 cm2 showed that those containing 5 % and 10 % CIP exhibited bactericidal activity against Escherichia coli and Staphylococcus aureus. Moreover, Human dermal fibroblast cells were compatible with the fabricated mats, as confirmed by the MTT assay. Finally, drug-loaded mats had a positive effect on wound healing in a scratch test, and mats with 10 % CIP and 2 g/L DFO showed the highest effect on promoting wound healing, indicating potential for use as a wound dressing.

Effects of plasma electrolytic oxidation process on the mechanical properties of additively manufactured porous biomaterials.

Metallic porous biomaterials are recently attracting more attention thanks to the additive manufacturing techniques which help produce more complex structures as compared to conventional techniques. On the other hand, bio-functional surfaces on metallic biomaterials such as titanium and its alloys are necessary to enhance the biological interactions with the host tissue. This study discusses the effect of plasma electrolytic oxidation (PEO), as a surface modification technique to produce bio-functional layers, on the mechanical properties of additively manufactured Ti6Al4V scaffolds based on the cubic unit cell. For this purpose, the PEO process with two different oxidation times was applied on scaffolds with four different values of relative density. The effects of the PEO process were studied by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), optical microscopy as well as static and dynamic (fatigue) mechanical testing under compression. SEM results indicated pore formation on the surface of the scaffolds after oxidation with a thickness of 4.85±0.36µm of the oxide layer after 2min and 9.04±2.27µm after 5min oxidation (based on optical images). The static test results showed the high effect of relative density of porous structure on its mechanical properties. However, oxidation did not influence most of the mechanical properties such as maximum stress, yield stress, plateau stress, and energy absorption, although its effect on the elastic modulus was considerable. Under fatigue loading, none of the scaffolds failed even after 106 loading cycles at 70% of their yield stress.