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1.
Sci Rep ; 12(1): 1846, 2022 02 03.
Artigo em Inglês | MEDLINE | ID: mdl-35115632

RESUMO

Adolescent idiopathic scoliosis (AIS) is the most prevalent pediatric spinal deformity. We previously demonstrated elongated cilia and an altered molecular mechanosensory response in AIS osteoblasts. The purpose of this exploratory study was to characterize the mechanosensory defect occurring in AIS osteoblasts. We found that cilia length dynamics in response to flow significantly differ in AIS osteoblasts compared to control cells. In addition, strain-induced rearrangement of actin filaments was compromised resulting in a failure of AIS osteoblasts to position or elongate in function of the bidirectional-applied flow. Contrary to control osteoblasts, fluid flow had an inhibitory effect on AIS cell migration. Moreover, flow induced an increase in secreted VEGF-A and PGE2 in control but not AIS cells. Collectively our data demonstrated that in addition to the observed primary cilium defects, there are cytoskeletal abnormalities correlated to impaired mechanotransduction in AIS. Thus, we propose that the AIS etiology could be a result of generalized defects in cellular mechanotransduction given that an adolescent growing spine is under constant stimulation for growth and bone remodeling in response to applied mechanical forces. Recognition of an altered mechanotransduction as part of the AIS pathomechanism must be considered in the conception and development of more effective bracing treatments.


Assuntos
Citoesqueleto de Actina/metabolismo , Cílios/metabolismo , Mecanotransdução Celular , Osteoblastos/metabolismo , Escoliose/metabolismo , Coluna Vertebral/metabolismo , Citoesqueleto de Actina/patologia , Adolescente , Braquetes , Estudos de Casos e Controles , Movimento Celular , Células Cultivadas , Criança , Cílios/patologia , Dinoprostona/metabolismo , Feminino , Humanos , Osteoblastos/patologia , Escoliose/patologia , Escoliose/terapia , Coluna Vertebral/patologia , Estresse Mecânico , Fator A de Crescimento do Endotélio Vascular/metabolismo
2.
Sci Rep ; 9(1): 10074, 2019 07 11.
Artigo em Inglês | MEDLINE | ID: mdl-31296888

RESUMO

Adolescent idiopathic scoliosis is the most prevalent spine deformity and the molecular mechanisms underlying its pathophysiology remain poorly understood. We have previously found a differential impairment of melatonin receptor signaling in AIS osteoblasts allowing the classification of patients into three biological endophenotypes or functional groups (FG1, FG2 and FG3). Here, we provide evidence that the defect characterizing each endophenotype lies at the level of Gαi proteins leading to a systemic and generalized differential impairment of Gi-coupled receptor signaling. The three Gαi isoforms exhibited a selective serine phosphorylation patterns for each AIS endophenotype resulting in a differential reduction in Gαi protein activity as determined by cellular dielectric spectroscopy and small interfering RNA methods. We found that one endophenotype (FG2) with phosphorylated Gαi1 and Gαi2 was consistently associated with a significantly high risk of spinal deformity progression when compared to the other two endophenotypes (FG1 and FG3). We further demonstrated that each endophenotype is conserved among affected family members. This study expands our understanding of the mechanism underlying the Gi-coupled receptor signaling dysfunction occurring in AIS and provides the first evidence for its hereditary nature. Collectively, our findings offers a new perspective on Gαi hypofunctionality in a human disease by revealing specific serine phosphorylation signatures of Gαi isoforms that may facilitate the identification of AIS patients at risk of spinal deformity progression.


Assuntos
Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP/metabolismo , Osteoblastos/metabolismo , Receptores de Melatonina/metabolismo , Escoliose/metabolismo , Adolescente , Células Cultivadas , Criança , Estudos de Coortes , Progressão da Doença , Feminino , Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP/genética , Humanos , Masculino , Fenótipo , Prognóstico , Isoformas de Proteínas/genética , RNA Interferente Pequeno/genética , Risco , Escoliose/genética , Transdução de Sinais
3.
Sci Rep ; 9(1): 5712, 2019 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-30952886

RESUMO

The cellular and molecular mechanisms underlying spinal deformity progression in adolescent idiopathic scoliosis (AIS) remain poorly understood. In this study, 804 French-Canadian patients and 278 age- and sex-matched controls were enrolled and genotyped for 12 single nucleotide polymorphisms (SNPs) in the chitinase 3-like 1 (CHI3L1) gene or its promoter. The plasma YKL-40 levels were determined by ELISA. We showed that elevation of circulating YKL-40 levels was correlated with a reduction of spinal deformity progression risk. We further identified significant associations of multiple CHI3L1 SNPs and their haplotypes with plasma YKL-40 levels and scoliosis severity as a function of their classification in a specific endophenotype. In the endophenotype FG3 group, we found that patients harboring the haplotype G-G-A-G-G-A (rs880633|rs1538372|rs4950881|rs10399805|rs6691378|rs946261), which presented in 48% of the cases, showed a positive correlation with the plasma YKL-40 levels (P = 7.6 × 10-6 and coefficient = 36). Conversely, the haplotype A-A-G-G-G-G, which presented in 15% of the analyzed subjects, showed a strong negative association with the plasma YKL-40 levels (P = 2 × 10-9 and coefficient = -9.56). We found that this haplotype showed the strongest association with AIS patients in endophenotype FG2 (P = 9.9 × 10-6 and coefficient = -13.53), who more often develop severe scoliosis compared to those classified in the other two endophenotypes. Of note, it showed stronger association in females (P = 1.6 × 10-7 and coefficient = -10.08) than males (P = 0.0021 and coefficient = -9.01). At the functional level, we showed that YKL-40 treatments rescued Gi-coupled receptor signalling dysfunction occurring in primary AIS osteoblasts. Collectively, our findings reveal a novel role for YKL-40 in AIS pathogenesis and a new molecular mechanism interfering with spinal deformity progression.


Assuntos
Proteína 1 Semelhante à Quitinase-3/sangue , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , Escoliose/sangue , Adolescente , Canadá , Proteína 1 Semelhante à Quitinase-3/genética , Feminino , Ligação Genética , Haplótipos , Humanos , Masculino , Escoliose/genética
4.
Sci Rep ; 7: 44260, 2017 03 14.
Artigo em Inglês | MEDLINE | ID: mdl-28290481

RESUMO

The primary cilium is an outward projecting antenna-like organelle with an important role in bone mechanotransduction. The capacity to sense mechanical stimuli can affect important cellular and molecular aspects of bone tissue. Idiopathic scoliosis (IS) is a complex pediatric disease of unknown cause, defined by abnormal spinal curvatures. We demonstrate significant elongation of primary cilia in IS patient bone cells. In response to mechanical stimulation, these IS cells differentially express osteogenic factors, mechanosensitive genes, and signaling genes. Considering that numerous ciliary genes are associated with a scoliosis phenotype, among ciliopathies and knockout animal models, we expected IS patients to have an accumulation of rare variants in ciliary genes. Instead, our SKAT-O analysis of whole exomes showed an enrichment among IS patients for rare variants in genes with a role in cellular mechanotransduction. Our data indicates defective cilia in IS bone cells, which may be linked to heterogeneous gene variants pertaining to cellular mechanotransduction.


Assuntos
Cílios/genética , Regulação da Expressão Gênica , Mecanotransdução Celular/genética , Osteoblastos/metabolismo , Osteogênese/genética , Escoliose/genética , Adolescente , Osso e Ossos/metabolismo , Osso e Ossos/patologia , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Moléculas de Adesão Celular/genética , Moléculas de Adesão Celular/metabolismo , Cílios/metabolismo , Cílios/patologia , Exoma , Feminino , Fator 3 de Crescimento de Fibroblastos/genética , Fator 3 de Crescimento de Fibroblastos/metabolismo , Perfilação da Expressão Gênica , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Humanos , Cinesinas/genética , Cinesinas/metabolismo , Osteoblastos/patologia , Fatores de Transcrição Box Pareados/genética , Fatores de Transcrição Box Pareados/metabolismo , Cultura Primária de Células , Receptores Proteína Tirosina Quinases/genética , Receptores Proteína Tirosina Quinases/metabolismo , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo , Escoliose/metabolismo , Escoliose/patologia , Proteínas com Domínio T/genética , Proteínas com Domínio T/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
5.
Spine (Phila Pa 1976) ; 40(8): 537-43, 2015 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-25646748

RESUMO

STUDY DESIGN: A replication association study that used genomic data generated from French-Canadian case and control cohorts. OBJECTIVES: To determine whether the 53 single nucleotide polymorphisms (SNPs) that were previously associated with spinal deformity progression in an American Caucasian cohort are similarly associated in French-Canadian population. SUMMARY OF BACKGROUND DATA: It is widely accepted that genetic factors contribute to adolescent idiopathic scoliosis. The identification of genetic variants associated with the predisposition or progression of curvature could facilitate diagnostic/prognostic tool development. Although 53 SNPs have been associated with spinal curve progression in Caucasian cohorts in the United States, these associations were not replicated in a large Japanese population study, arguing that such a discrepancy could be explained by ethnicity, thus raising the importance of a replication study in an independent Caucasian population of European descent. METHODS: Genomic data were collected from the French-Canadian population, using the Illumina HumanOmni 2.5M BeadChip. Fifty-two SNPs, tested in ScoliScore or in high linkage disequilibrium with SNPs in the test, were selected to assess their association with scoliosis generally, and with spinal curve progression. One SNP in ScoliScore, rs16909285, could not be evaluated in our Genome-Wide association study. RESULTS: None of the SNPs used in ScoliScore were associated with adolescent idiopathic scoliosis curve progression or curve occurrence in French-Canadian population. We evaluated 52 SNPs in severe patients by comparing risk allele frequencies with those in nonsevere patients and with those in control individuals. There was no significant difference between the severe group and the nonsevere group or between the severe group and the control group. CONCLUSION: Although the 52 SNPs studied here were previously associated with curve progression in an American population of European descent, we found no association in French-Canadian patients with adolescent idiopathic scoliosis. This second replication cohort suggests that the lack of association of these SNPs in a Japanese cohort is not due to ethnicity. LEVEL OF EVIDENCE: 4.


Assuntos
Estudos de Associação Genética , Escoliose/genética , População Branca/genética , Adolescente , Adulto , Estudos de Casos e Controles , Criança , Progressão da Doença , Feminino , Frequência do Gene , Genótipo , Humanos , Desequilíbrio de Ligação , Masculino , Polimorfismo de Nucleotídeo Único , Quebeque , Fatores de Risco , Índice de Gravidade de Doença , Adulto Jovem
6.
BMC Genet ; 12: 16, 2011 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-21269476

RESUMO

BACKGROUND: Understanding the genetic basis of heritable spinal curvature would benefit medicine and aquaculture. Heritable spinal curvature among otherwise healthy children (i.e. Idiopathic Scoliosis and Scheuermann kyphosis) accounts for more than 80% of all spinal curvatures and imposes a substantial healthcare cost through bracing, hospitalizations, surgery, and chronic back pain. In aquaculture, the prevalence of heritable spinal curvature can reach as high as 80% of a stock, and thus imposes a substantial cost through production losses. The genetic basis of heritable spinal curvature is unknown and so the objective of this work is to identify quantitative trait loci (QTL) affecting heritable spinal curvature in the curveback guppy. Prior work with curveback has demonstrated phenotypic parallels to human idiopathic-type scoliosis, suggesting shared biological pathways for the deformity. RESULTS: A major effect QTL that acts in a recessive manner and accounts for curve susceptibility was detected in an initial mapping cross on LG 14. In a second cross, we confirmed this susceptibility locus and fine mapped it to a 5 cM region that explains 82.6% of the total phenotypic variance. CONCLUSIONS: We identify a major QTL that controls susceptibility to curvature. This locus contains over 100 genes, including MTNR1B, a candidate gene for human idiopathic scoliosis. The identification of genes associated with heritable spinal curvature in the curveback guppy has the potential to elucidate the biological basis of spinal curvature among humans and economically important teleosts.


Assuntos
Curvaturas da Coluna Vertebral/genética , Animais , Mapeamento Cromossômico , Predisposição Genética para Doença , Poecilia/genética , Polimorfismo de Nucleotídeo Único , Locos de Características Quantitativas
7.
Scoliosis ; 5: 10, 2010 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-20529276

RESUMO

BACKGROUND: The curveback lineage of guppy is characterized by heritable idiopathic-type spinal curvature that develops during growth. Prior work has revealed several important developmental similarities to the human idiopathic scoliosis (IS) syndrome. In this study we investigate structural and histological aspects of the vertebrae that are associated with spinal curvature in the curveback guppy and test for sexual dimorphism that might explain a female bias for severe curve magnitudes in the population. METHODS: Vertebrae were studied from whole-mount skeletal specimens of curved and non-curved adult males and females. A series of ratios were used to characterize structural aspects of each vertebra. A three-way analysis of variance tested for effects of sex, curvature, vertebral position along the spine, and all 2-way interactions (i.e., sex and curvature, sex and vertebra position, and vertebra position and curvature). Histological analyses were used to characterize micro-architectural changes in affected vertebrae and the intervertebral region. RESULTS: In curveback, vertebrae that are associated with curvature demonstrate asymmetric shape distortion, migration of the intervertebral ligament, and vertebral thickening on the concave side of curvature. There is sexual dimorphism among curved individuals such that for several vertebrae, females have more slender vertebrae than do males. Also, in the region of the spine where lordosis typically occurs, curved and non-curved females have a reduced width at the middle of their vertebrae, relative to males. CONCLUSIONS: Based on similarities to human spinal curvatures and to animals with induced curves, the concave-convex biases described in the guppy suggest that there is a mechanical component to curve pathogenesis in curveback. Because idiopathic-type curvature in curveback is primarily a sagittal deformity, it is structurally more similar to Scheuermann kyphosis than IS. Anatomical differences between teleosts and humans make direct biomechanical comparisons difficult. However, study of basic biological systems involved in idiopathic-type spinal curvature in curveback may provide insight into the relationship between a predisposing aetiology, growth, and biomechanics. Further work is needed to clarify whether observed sex differences in vertebral characteristics are related to the female bias for severe curves that is observed in the population.

8.
Spine (Phila Pa 1976) ; 35(5): 511-6, 2010 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-20147879

RESUMO

STUDY DESIGN: A comparative allometric study of body lengths in an animal model for human idiopathic-type scoliosis. OBJECTIVE: To compare body length variation among adult curved and noncurved curveback female guppies. SUMMARY OF BACKGROUND DATA: Tallness and/or abnormal anthropometric parameters have been correlated to idiopathic-type scoliosis (IS) in numerous studies. Heritable curvature in curveback has demonstrated morphologic and developmental similarities to human IS. Because control of body length in the guppy is heritable and variable, we investigated whether length might also be correlated to curvature in the curveback population. METHODS: Component body lengths were measured from digital photographs for 321 (246 curved and 75 noncurved) females. Sources of experimental variation were omitted by only measuring 2-dimensional curves in mature females all from the same pedigree, and raised under controlled conditions of diet and environment. Body length was divided into 2 component parts (precaudal and caudal). Body lengths were tested statistically for correlation to curvature and curve magnitude. RESULTS: Although absolute length does not correlate to curvature, this survey of length in the curveback model reveals 2 important similarities to anthropometric studies of IS: that there are disproportionate body lengths among females with curvature, and the suggestion of an underlying growth abnormality among curved individuals. CONCLUSION: In order to better characterize the relationship between growth, length disproportion, and curvature in the guppy, further studies are warranted. However, this inquiry further supports the usefulness of curveback as a model for understanding the basic biology of idiopathic-type scoliosis and encourages study of growth-related factors.


Assuntos
Poecilia/anatomia & histologia , Curvaturas da Coluna Vertebral/patologia , Análise de Variância , Animais , Biometria , Feminino , Poecilia/genética , Análise de Regressão , Curvaturas da Coluna Vertebral/genética
9.
Med Hypotheses ; 72(3): 348-52, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19070438

RESUMO

Human familial/idiopathic-type scoliosis (IS) is a complex genetic disorder for which the cause is unknown. The curve phenotype characteristically demonstrates pronounced morphological and developmental variability that is likely a consequence of biomechanical, environmental, and genetic differences between individuals. In addition, risk factors that affect the propensity for curves to progress to severity are unknown. Progress in understanding the fundamental biology of idiopathic-type scoliosis has been limited by the lack of a genetic/developmental animal model. Prior to consideration of teleosts, developmental idiopathic-type scoliosis has been considered to be exclusive to humans. Consequently, there is the notion that the syndrome is a result of bipedalism, and many studies try to explain the deformity from this anthrocentric viewpoint. This perspective has been reinforced by the choice of animals used for study, in that chickens and bipedal rats and mice demonstrate idiopathic-type curvature when made melatonin-deficient, but quadrupedal animals do not. Overlooked is the fact that teleosts also demonstrate similar curvature when made melatonin-deficient. Our characterization of the guppy curveback has demonstrated that non-induced idiopathic-type curvature is not exclusive to humans, nor bipedalism. We hypothesize that unique morphological, developmental and genetic parallels between the human and guppy syndromes are due to common molecular pathways involved in the etiopathogenesis of both phenotypes. We explore established gene conservation between human and teleost genomes that are in pathways hypothesized to be involved in the IS syndrome. We present non-induced vertebral wedging as a unique shared feature in IS and curveback that suggests a similar interaction between a molecular phenotype on the level of the vertebral anatomy, and biomechanics. We propose that rather than bipedalism per se, expression of idiopathic-type scoliosis is dependent on normal spinal loading applied along the cranio-caudal axis that interacts with an unknown factor causing the primary curve. In this regard, a comparative biological approach using a simplified teleost model will promote discovery of basic processes integral to idiopathic-type scoliosis in teleosts and humans, and highlight human-specific aspects of the deformity.


Assuntos
Marcha/genética , Locomoção/genética , Poecilia/genética , Escoliose/genética , Escoliose/veterinária , Coluna Vertebral/fisiopatologia , Animais , Predisposição Genética para Doença/genética , Humanos , Especificidade da Espécie
10.
Spine (Phila Pa 1976) ; 32(7): 735-41, 2007 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-17414906

RESUMO

STUDY DESIGN: This study investigated the morphology, pathogenesis, and inheritance of idiopathic-like spinal curvature in the guppy syndrome, curveback. OBJECTIVE: To determine whether curveback could be applied as a model for the primary factors that contribute to heritable spinal curvature in humans, specifically, the etiopathogenesis of human familial idiopathic scoliosis. SUMMARY OF BACKGROUND DATA: Although a genetic basis is accepted, phenotypic complexity and the lack of an animal model with noninduced curvature have made identification of idiopathic scoliosis etiology difficult. It is well established that humans and fish share many genes with similar tissue and temporal expression characteristics, and comparisons between human and fish genomes have proven to be valuable for understanding the genetics of diseases affecting humans. METHODS: The curveback lineage of guppies was constructed from a single curved male crossed to a normal female. Offspring (103) from the original cross were scored from birth until death for the presence and magnitude of spinal curvature. Genetic architecture was investigated through selective inbreeding, analysis of the distribution of curve magnitude in the mature population, and assessment of curve dynamics during development. Computed tomography assessed vertebral detail. RESULTS: Computed tomography reveals that vertebral breakage or fusion is not associated with the curveback syndrome. Inbreeding demonstrates a strong genetic influence on curveback, and the distribution of curve magnitude among adult fish suggests polygenic inheritance. There is a female bias for curves of high magnitude and curves that resolve before maturity. There is developmental variability for the age of curve onset, curve progression, and final curve magnitude. CONCLUSIONS: Observed parallels between the curveback syndrome and human idiopathic scoliosis suggest that the guppy model is an unexploited resource for the identification of primary etiological factors involved in curvature. As models for biomedical research, teleosts offer great potential regarding spinal stability and deformity.


Assuntos
Doenças dos Peixes/genética , Mutação/genética , Poecilia/genética , Curvaturas da Coluna Vertebral/veterinária , Animais , Fenômenos Biomecânicos , Modelos Animais de Doenças , Doenças dos Peixes/patologia , Humanos , Endogamia , Fenótipo , Escoliose/genética , Escoliose/patologia , Curvaturas da Coluna Vertebral/genética , Curvaturas da Coluna Vertebral/patologia
11.
Artigo em Inglês | MEDLINE | ID: mdl-17240199

RESUMO

Vertebral development is a dynamic and complicated process, and defects can be caused by a variety of influences. Spinal curvature with no known cause (idiopathic scoliosis) affects 2-3% of the human population. In order to understand the etiology and pathogenesis of complex human skeletal defects such as idiopathic scoliosis, multiple models must be used to study all of the factors affecting vertebral stability and deformity. Although fish and humans have many of the same types of offenses to vertebral integrity, they have been overlooked as a resource for study. The most common morphological deformity reported for fish are those that occur during the development of the spinal system, and as with humans, curvature is a common morphological consequence. Here we review spinal curvature in teleosts and suggest that they are an unexploited resource for understanding the basic elements of vertebral stability, deformity, development and genetics. Fish can be a value to vertebral research because they are tractable, have a diversity of non-induced vertebral deformities, and substantial genomic resources. Current animal models lack non-induced deformities and the experimental tractability necessary for genetic studies. The fact that fish are free of an appendicular skeleton should allow for analysis of basic spinal integrity without the biomechanical constraints observed in quadrupedal and bipedal models. To illustrate the point we review human idiopathic scoliosis and the potential contribution teleosts can make for the identification of causes, risk factors, and treatment options.


Assuntos
Peixes/fisiologia , Coluna Vertebral/anormalidades , Coluna Vertebral/fisiologia , Animais , Humanos , Escoliose/congênito , Escoliose/patologia , Curvaturas da Coluna Vertebral/congênito , Curvaturas da Coluna Vertebral/patologia , Doenças da Coluna Vertebral/congênito , Doenças da Coluna Vertebral/patologia , Coluna Vertebral/crescimento & desenvolvimento , Coluna Vertebral/patologia
12.
PLoS One ; 1: e76, 2006 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-17183708

RESUMO

Hsp90 controls dramatic phenotypic transitions in a wide array of morphological features of many organisms. The genetic-background dependence of specific abnormalities and their response to laboratory selection suggested Hsp90 could be an 'evolutionary capacitor', allowing developmental variation to accumulate as neutral alleles under normal conditions and manifest selectable morphological differences during environmental stress. The relevance of Hsp90-buffered variation for evolution has been most often challenged by the idea that large morphological changes controlled by Hsp90 are unconditionally deleterious. To address this issue, we tested an Hsp90-buffered abnormality in Drosophila for unselected pleiotropic effects and correlated fitness costs. Up to 120-fold differences in penetrance among six highly related selection lines, started from an initially small number of flies and rapidly selected for and against a deformed eye trait (dfe), did not translate into measurable differences in any of several tests of viability, lifespan or competitive fitness. Nor were 17 dfe Quantitative Trait Loci (QTL) associated with fitness effects in over 1,400 recombinant lines. Our ability to detect measurable effects of inbreeding, media environment and the white mutation in the selection line backgrounds independent of dfe penetrance suggests that, within the limitations of laboratory tests of fitness, this large morphological change controlled by Hsp90 was selectable independent of strong, correlated and unconditionally deleterious effects--abundant, polygenic variation hidden by Hsp90 allows potentially deleterious alleles to be readily replaced during selection by less deleterious alleles with similar phenotypic effects. Hsp90 links environmental stress with the expression of developmental variation controlling unprecedented morphological plasticity. As outlined here and in the companion paper of this issue, the complex genetic architecture of Hsp90-buffered variation supports a remarkable modularity of Hsp90 effects on quantitative and qualitative phenotypes, consistent with the 'Hsp90 capacitor hypothesis' and standard quantitative genetic models of threshold traits.


Assuntos
Proteínas de Drosophila/genética , Drosophila/genética , Evolução Molecular , Proteínas de Choque Térmico HSP90/genética , Alelos , Animais , Animais Geneticamente Modificados , Drosophila/fisiologia , Anormalidades do Olho/genética , Feminino , Fertilidade/genética , Genes de Insetos , Variação Genética , Longevidade/genética , Masculino , Modelos Genéticos , Mutação , Penetrância , Fenótipo , Locos de Características Quantitativas
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