[Ion-exchange properties of immobilized DNA: influence of the polymer concentration and the solvent nature on the exchange of alkali metals]. / Ionoobmennye svoistva immobilizovannoi DNK: vliianie kontsentratsii polimera i prirody rastvoriteliia na obmen ionov shchelochnykh metallov.

The method of ion exchange on immobilized DNA, which allows to determine quantitative parameters of ion binding with a high precision, is used for studying of DNA ion selectivity. Insoluble ion exchangers on the DNA basis with the exchange capacity of 0.09 and 0.17 mg-equiv. per 1 g of dry gel are synthesized by means of immobilization of DNA gel in polyacrylamide gel. Constants of ion-exchange equilibrium for the exchanges K+-Na+ and K+-Li+ are determined on these exchangers in water and 50% water-dioxane solution. It is shown that DNA binds selectively only Li+. The selectivity to Li+ increases with the increase of DNA concentration in gel. The specific properties of Li+-DNA in solutions and in the solid state, for example, the impossibility of the B-A transition, are discussed. The selectivity reversal in favor of K+ is observed in water-dioxane solution. The cause of the selectivity reversal and the question of possible participation of cell polyelectrolytes in creation of ion gradients in the living cell are discussed.

Investigation of vibrational relaxation in the hydrogen chloride chemical laser.

The gain dependence on time of the HCl chemical laser was measured and calculated. Comparison of the calculated and measured results enables one to determine the constant of HCl-HCl deactivation which is found to be within (0.2-9) 10(12) cm(3) mole(-1) sec(-1).