Study on the accelerated biodegradation of PAHs in subsurface soil via coupled low-temperature thermally treatment and electron acceptor stimulation based on metagenomic sequencing.

In situ anoxic bioremediation is a sustainable technology to remediate PAHs contaminated soils. However, the limited degradation rate of PAHs under anoxic conditions has become the primary bottleneck hindering the application of this technology. In this study, coupled low-temperature thermally treatment (<50 °C) and EA biostimulation was used to enhance PAH removal. Anoxic biodegradation of PAHs in soil was explored in microcosms in the absence and presence of added EAs at 3 temperatures (15 °C, 30 °C, and 45 °C). The influence of temperature, EA, and their interaction on the removal of PAHs were identified. A PAH degradation model based on PLSR analysis identified the importance and the positive/negative role of parameters on PAH removal. Soil archaeal and bacterial communities showed similar succession patterns, the impact of temperature was greater than that of EA. Soil microbial community and function were more influenced by temperature than EAs. Close and frequent interactions were observed among soil bacteria, archaea, PAH-degrading genes and methanogenic genes. A total of 15 bacterial OTUs, 1 PAH-degrading gene and 2 methanogenic genes were identified as keystones in the network. Coupled low-temperature thermally treatment and EA stimulation resulted in higher PAH removal efficiencies than EA stimulation alone and low-temperature thermally treatment alone.

Influencing factors identification and the nested structure analysis of heavy metals in soils in entire city and surrounding the multiple pollution sources.

Identifying the sources of pollutants and analyzing the nested structure of heavy metals is vital for the prevention and control of soil pollution. However, there is a lack of research on comparison the main sources and the nested structure at different scales. In this study, two spatial extent scales were taken as the research objects, the results showed that, (1) the point exceeding standard rate of As, Cr, Ni, and Pb is higher at the entire city scale; (2) As and Pb, while Cr, Ni, and Zn, have weaker spatial variability at the entire scale and surrounding the pollution sources, respectively; (3) the contribution of the larger structure of Cr and Ni, while Cr, Ni, and Zn, at the entire scale and surrounding the pollution sources, respectively, is bigger to the total variability. The representation of semivariogram is better when the general spatial variability is weaker and the contribution of the smaller structure is lower; (4) various factors with different influencing distance could lead to nested structure even at a small extent spatial scale. The results provide a basis for the determination of remediation and prevention objectives at different spatial scales.

Polycyclic aromatic hydrocarbon removal from subsurface soil mediated by bacteria and archaea under methanogenic conditions: Performance and mechanisms.

In situ anoxic bioremediation is an easy-to-use technology to remediate polycyclic aromatic hydrocarbon (PAH)-contaminated soil. Degradation of PAHs mediated by soil bacteria and archaea using CO2 as the electron acceptor is an important process for eliminating PAHs under methanogenic conditions; however, knowledge of the performance and mechanisms involved is poorly unveiled. In this study, the effectiveness and efficiency of NaHCO3 (CO2) as an electron acceptor to stimulate the degradation of PAHs by bacteria and archaea in highly contaminated soil were investigated. The results showed that CO2 addition (EC2000) promoted PAH degradation compared to soil without added CO2 (EC0), with 4.18%, 9.01%-8.05%, and 6.19%-12.45% increases for 2-, 3- and 4-ring PAHs after 250 days of incubation, respectively. Soil bacterial abundances increased with increasing incubation time, especially for EC2000 (2.90 × 108 g-1 soil higher than EC0, p < 0.05). Different succession patterns of the soil bacterial and archaeal communities during PAH degradation were observed. According to the PCoA and ANOSIM results, the soil bacterial communities were greatly (ANOSIM: R = 0.7232, P = 0.001) impacted by electron acceptors, whereas significant differences in the archaeal communities were not observed (ANOSIM: R = 0.553, P = 0.001). Soil bacterial and archaeal co-occurrence network analyses showed that positive correlations outnumbered the negative correlations throughout the incubation period for both treatments (e.g., EC0 and EC2000), suggesting the prevalence of coexistence/cooperation within and between these two domains rather than competition. The higher complexity, connectance, edge, and node numbers in EC2000 revealed stronger linkage and a more stable co-occurrence network compared to EC0. The results of this study could improve the knowledge on the removal of PAHs and the responses of soil bacteria and archaea to CO2 application, as well as a scientific basis for the in situ anoxic bioremediation of PAH-contaminated industrial sites.

Insights into the effects of Fenton oxidation on PAH removal and indigenous bacteria in aged subsurface soil.

Combined chemical oxidation and bioremediation is a promising method of treating polycyclic aromatic hydrocarbon (PAH) contaminated soil, wherein indigenous soil bacteria play a critical role in the subsequent biodegradation of PAHs after the depletion of the oxidant. In this study, different Fenton conditions were applied by varying either the oxidation mode (conventional Fenton (CF), Fenton-like (LF), modified Fenton (MF), and graded modified Fenton (GMF)) or the H2O2 dosage (0%, 3%, 6%, and 10% (v/v)) to treat PAH contaminated soil. The results revealed that when equal dosages of H2O2 are applied, PAHs are significantly removed following oxidation treatment, and the removal percentages obeyed the following sequence: CF > GMF > MF > LF. In addition, higher dosages of H2O2 improved the PAH removal from soil treated with the same oxidation mode. The ranges of total PAHs removal efficiencies in the soil added 3%, 6%, and 10% of H2O2 (v/v) were 18.04%∼59.48%, 31.88%∼71.83%, and 47.56%∼78.16%, respectively. The PAH removal efficiency decreased with increasing ring numbers for the same oxidation treatment. However, the negative influences on soil bacterial abundance, community composition, and function were observed after Fenton treatment. After Fenton oxidation, the bacterial abundance in the soil received 3%, 6%, and 10% of H2O2 (v/v) decreased 1.96-2.69, 2.44-3.22, and 3.09-3.42 orders of magnitude compared to the untreated soil. The soil bacterial abundance tended to be impacted by the oxidation mode and H2O2 dosage simultaneously. While the main factor influencing the soil bacterial community composition was the H2O2 dosages. The results of this study showed that different oxidation mode and H2O2 dosage exhibited different effects on PAHs removal and soil bacteria (including abundance, community composition, and function), and there was a trade-off between the removal of PAHs and the adverse impact on soil bacteria.

Toxicity reduction of reverse osmosis concentrates from petrochemical wastewater by electrocoagulation and Fered-Fenton treatments.

In this work, both Electrocoagulation (EC) and Fered-Fenton (FF) technologies were used to treat reverse osmosis concentrates (ROC) from petrochemical production. The toxicity reduction capacity and mechanism were comparatively assessed during these two treatments. The results showed that FF exhibited higher capacity to reduce toxicity than EC in the 30 min treatment, which could be attributed to the removal of organic pollutants and heavy metals. The results showed that the ROC contained organics with molecular weight of 1200 g mol-1 and 220 g mol-1, which mainly consisted of the soluble microbial by-product-like and humic acid-like substances. The removal of these organics directly led to the noticeable toxicity reduction. Alkanes, haloalkanes, ketones, PAHs, and other four organic pollutants were the dominant species in the ROC, and the removal of small molecular weight organic pollutants played an essential role in reducing toxicity. FF exhibited stronger capacity to remove PAHs, BTEXS and haloalkanes, and the removal efficiencies for the PAHs were in the following order: 5-ring > 4-ring > 3-ring > 2-ring. The promotion of heavy metals removal appeared to be favorable for decreasing toxicity in ROC. This study illustrated the mechanism of the toxicity reduction and the characteristics of pollutants removal during FF and EC treatments, and provided valuable guidance for petrochemical manufacturing to the toxicity reduction and operation of wastewater treatment facilities.

An effective method for determining the optimal sampling scale based on the purposes of soil pollution investigations and the factors influencing the pollutants.

There is a lack of a systematic method for determining the optimal sampling scale based on the purposes of soil pollution investigations (purposeinvest) and the factors influencing of pollutants, which could affect the accuracy of determining pollution scope of the pollution. Therefore, in this study, both the purposeinvest and the influencing factors were considered to determine the optimal sampling scale. The conclusions were obtained through geostatistical and spatial analysis. (1) The optimal sampling scale should account for 3% of the range of the pollutants, which can identify pollution information and minimize sampling costs. (2) The optimal sampling scale should be set to 3% of the range of the main factor influencing the pollutants in the absence of prior pollution information. (3) The greater the influences of the factors on the pollutants, the closer the optimal sampling scale calculated according to the influencing factors will be to that calculated based on the purposeinvest. (4) The method of determination based on both the purposeinvest and the influencing factors was concluded to be rational and reliable based on validation and advantage analysis. These results provide a method for soil pollution investigation that can minimize costs and improve the representativeness of the sample sites.

Quantitative analysis of the main sources of pollutants in the soils around key areas based on the positive matrix factorization method.

Quantitative identification of the main sources of heavy metals and polycyclic aromatic hydrocarbons (PAHs) in soils around multiple types of key areas is of great significance for blocking pollution sources. However, there is a lack of more comprehensive relevant research. In this study, Beijing was taken as the research area and four main sources were identified using the positive matrix factorization (PMF) method. The concentration of Pb, PAHs, Cr, and Hg in soils was significantly affected by the presence of landuse type, road traffic, natural factor, and industrial production, respectively, and the farmland, distance to main road, Proterozoic Changcheng-Jixian parent material and cinnamon soil type, and the gross industrial production make greater contributions to these four factors respectively than other variables. Moreover, the uncertainty of the PMF indicates that this four-factor PMF solution is stable and appropriate. These results provide support for the comprehensive control of soil environmental risks.

Removal of polycyclic aromatic hydrocarbons (PAHs) and the response of indigenous bacteria in highly contaminated aged soil after persulfate oxidation.

Integrated chemical-biological treatment is a promising alternative to remove PAHs from contaminated soil, wherein indigenous bacteria is the key factor for the biodegradation of residual PAHs after the application of chemical oxidation. However, systematical study on the impact of persulfate (PS) oxidation on indigenous bacteria as well as PAHs removal is still scarce. In this study, the influences of different PS dosages (1%, 3%, 6%, and 10% [w/w]), as well as various activation methods (native iron, H2O2, alkaline, ferrous iron, and heat) on PAHs removal and indigenous bacteria in highly contaminated aged soil were investigated. Apparent degradation of PAHs in the soil treated with PS oxidation was observed, and the removal efficiency of total PAHs in the soil ranged from 38.28% to 79.97%. The removal efficiency of total PAHs in the soil increased with increasing consumption of PS. However, the bacterial abundance in soil was negatively affected following oxidation for all of the treatments added with PS, with bacterial abundance in the soil decreased by 0.89-2.93 orders of magnitude compared to the untreated soil. Moreover, the number of total bacteria in the soil decreased as PS consumption increased. Different PS activation methods and PS dosages exhibited different influences on the bacterial community composition. Bacteria capable of degrading PAHs under anoxic conditions were composed predominantly by Proteobacteria and Firmicutes. The total amount of Proteobacteria and Firmicutes also decreased with increasing consumption of PS. The results of this study provide important insight for the design of PAHs contaminated soil remediation projects.