Ent-11α-Hydroxy-15-oxo-kaur-16-en-19-oic-acid Inhibits Growth of Human Lung Cancer A549 Cells by Arresting Cell Cycle and Triggering Apoptosis.

OBJECTIVE: To examine the apoptotic effect of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F), a compound isolated from Pteris semipinnata L (PsL), in human lung cancer A549 cells. METHODS: A549 cells were treated with 5F (0-80 µg/ml) for different time periods. Cytotoxicity was examined using a MTT method. Cell cycle was examined using propidium iodide staining. Apoptosis was examined using Hoechst 33258 staining, enzyme-linked immunosorbent assay (ELISA) and caspase-3 activity analysis. Expression of representative apoptosis-related proteins was evaluated by Western blot analysis. Reactive oxygen species (ROS) level was measured using standard protocols. Potential interaction of 5F with cisplatin was also examined. RESULTS: 5F inhibited the proliferation of A549 cells in a concentration- and time-dependent manner. 5F increased the accumulation of cells in sub-G1 phase and arrested the cells in the G2 phase. Exposure to 5F induced morphological changes and DNA fragmentation that are characteristic of apoptosis. The expression of p21 was increased. 5F exposure also increased Bax expression, release of cytochrome c and apoptosis inducing factor (AIF), and activation of caspase-3. 5F significantly sensitized the cells to cisplatin toxicity. Interestingly, treatment with 5F did not increase ROS, but reduced ROS production induced by cisplatin. CONCLUSION: 5F could inhibit the proliferation of A549 cells by arresting the cells in G2 phase and by inducing mitochondrial-mediated apoptosis.

Structural determination of two new triterpenoids biotransformed from glycyrrhetinic acid by Mucor polymorphosporus.

Five hydroxylated derivatives of glycyrrhetinic acid by Mucor polymorphosporus were isolated. Among them, 6beta, 7beta-dihydroxyglycyrrhentic acid (2) and 27-hydroxyglycyrrhentic acid (3) are new compounds. Their chemical structures were identified by spectral methods including 2D-NMR.

[Determination of chlorogenic acid and total caffeoylquinic acid in 6 Folium Lonicerae].

OBJECTIVE: To determine the contents of chlorogenic acid and total caffeoylquinic acid in 6 Folium Lonicerae in Sichuan province and Chongqing city for evaluating the quality of them. METHODS: The HPLC analysis and spectrophotometry analysis were used. RESULTS: The contents of chlorogenic acid and total caffeoylquinic acid in 6 Folium Lonicerae had been determined. CONCLUSION: The method is accurate, simple and has a good reproducibility. The results can be used as a reference for the reasonable medication, quality control and further study of Folium Lonicerae.