RESUMO
Neuroblastoma is associated with chromosomal aberrations of 1p and 1q in a majority of cases. Some nonrandom secondary changes were observed in this study. The role of these changes in the development and progression of neuroblastoma is examined. Chromosomal analysis was performed on 33 children with neuroblastoma using fine needle aspiration cultures. Metaphases were observed in 57.5% of cases. 86.6% showed the involvement of chromosome 1. Double minutes and unidentifiable markers was also observed. The most frequent secondary changes included add(4)(q35), add(11)(p13), add(14)(q32), add(16)(q12). add(17)(p13), add(19)(q12) and add(21)(q22). Minority of cases showed deletions and translocations. Ploidy pattern ranged from diploid to hypotetraploid. The present study substantiates aberration of chromosome 1 in the form of deletions, additions, duplications and iso-chromosome with some notable secondary abnormalities.
Assuntos
Biópsia/métodos , Aberrações Cromossômicas , Neuroblastoma/genética , Adolescente , Criança , Pré-Escolar , Deleção Cromossômica , Cromossomos Humanos Par 1 , Cromossomos Humanos Par 17 , Feminino , Deleção de Genes , Humanos , Lactente , Cariotipagem , Masculino , Metáfase , Ploidias , Translocação GenéticaRESUMO
Chromosomal analysis was performed in fine needle aspiration samples of 98 primary Ewing tumors (ETs) prior to treatment. Among the 58 (59.18%) successful cultures, t(11;22)(q24;q12) was observed in 87.9% and 6.8% had abnormalities other than t(11;22), viz., del(22)(q12), der(16)t(1;16)(q12;q11), and variant t(8;22)(q24;q12). Involvement of breakpoints 1q21, 1q22, 3p14, 16q22, and 17p13 was also observed. Numerical abnormalities such as trisomies 8 and 12 were found in 29.3% and 20.6% and trisomy 18 in 17.2%. An attempt was made to evaluate the role of these additional changes in the process of tumor development, metastasis, and progression of the disease. This is the largest cytogenetic study on ET from a single center using a simple and reliable technique of fine-needle aspiration culture. The literature on cytogenetics of ET is reviewed.
Assuntos
Neoplasias Ósseas/genética , Aberrações Cromossômicas/genética , Cromossomos Humanos 1-3/genética , Cromossomos Humanos 16-18/genética , Cromossomos Humanos Par 11/genética , Cromossomos Humanos Par 22/genética , Sarcoma de Ewing/genética , Adolescente , Adulto , Biópsia por Agulha , Neoplasias Ósseas/patologia , Criança , Pré-Escolar , Transtornos Cromossômicos , Citogenética , Feminino , Humanos , Masculino , Sarcoma de Ewing/patologiaRESUMO
Nuclear DNA content and cell cycle distribution in fresh tissues from 40 malignant and 10 benign breast tumors were assessed by flow cytometry using a DNA-specific fluorochrome, 4,6-diamidino-2phenyl-indole hydrochloride. DNA indices (DIs) (relative DNA content of tumor cells with reference to normal cells, 4,6-diamino-2phenyl-indole-hydrochloride ranged from 0.85 to 5.6 for malignant tumors and from 1.8 to 2.2 for benign tumors. Proliferating fraction (PF) (total cells in S and G2 + M phases) values were significantly higher in malignant tumors (35.4 +/- 14.75, mean +/- SD; P < .001) than in benign tumors (14.2 + 4.9) and adjacent normal tissues (6.6 + 2.73). DIs and cell cycle distribution correlated with clinicopathologic parameters, disease progression and survival. Four-year survival was greater in patients with a DI value of 1.8-2.2 as well as with SF values less than 10%.
