RESUMO
Sexual selection is a major evolutionary process, shaping organisms in terms of success in competition for access to mates and their gametes. The study of sexual selection has provided rich empirical and theoretical literature addressing the ecological and evolutionary causes and consequences of competition for gametes. However, there remains a bias towards individual, species-specific studies, whilst broader, cross-species comparisons looking for wider-ranging patterns in sexual selection remain uncommon. For instance, we are still some ways from understanding why particular kinds of traits tend to evolve under sexual selection, and under what circumstances. Here we consider sexual selection in the Heteroptera, a sub-order of the Hemiptera, or true bugs. The latter is the largest of the hemimetabolous insect orders, whilst the Heteroptera itself comprises some 40,000-plus described species. We focus on four key sexual signaling modes found in the Heteroptera: chemical signals, acoustic signaling via stridulation, vibrational (substrate) signaling, and finally tactile signaling (antennation). We compare how these modes vary across broad habitat types and provide a review of each type of signal. We ask how we might move towards a more predictive theory of sexual selection, that links mechanisms and targets of sexual selection to various ecologies.
RESUMO
The use of environmental DNA (eDNA) to monitor rare and elusive species has great potential for conservation biology. Traditional surveying methods can be time-consuming, labour-intensive, subject to error or can be invasive and potentially damaging to habitat. The Trinidad golden treefrog (Phytotriades auratus) is one such species that would benefit from such an approach. This species inhabits the giant bromeliad (Glomeropitcairnia erectiflora) on two peaks on the Caribbean island of Trinidad. Traditional survey methods for this species have required the destruction of the giant bromeliad, which is the only known habitat of this frog. Here we described the development of an eDNA PCR-based assay that uses water drawn from the water-filled phytotelmata of the giant bromeliad along with the use of a synthetic DNA positive control that can be easily amplified in the bacterium Escherichia coli. The assay can detect to a DNA concentration of 1.4ng. Sampling of 142 bromeliads using this method revealed 9% were positive for P. auratus DNA. These data suggest that eDNA methods also have great potential for revealing the presence of elusive species in arboreal habitats.
