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1.
Plant Biotechnol J ; 13(7): 875-83, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25487781

RESUMO

Host-induced gene silencing (HIGS) is an RNA interference-based approach in which small interfering RNAs (siRNAs) are produced in the host plant and subsequently move into the pathogen to silence pathogen genes. As a proof-of-concept, we generated stable transgenic lettuce plants expressing siRNAs targeting potentially vital genes of Bremia lactucae, a biotrophic oomycete that causes downy mildew, the most important disease of lettuce worldwide. Transgenic plants, expressing inverted repeats of fragments of either the Highly Abundant Message #34 (HAM34) or Cellulose Synthase (CES1) genes of B. lactucae, specifically suppressed expression of these genes, resulting in greatly reduced growth and inhibition of sporulation of B. lactucae. This demonstrates that HIGS can provide effective control of B. lactucae in lettuce; such control does not rely on ephemeral resistance conferred by major resistance genes and therefore offers new opportunities for durable control of diverse diseases in numerous crops.


Assuntos
Lactuca/microbiologia , Oomicetos/fisiologia , Doenças das Plantas/microbiologia , Plantas Geneticamente Modificadas/microbiologia , Inativação Gênica , Lactuca/genética , Oomicetos/genética , Doenças das Plantas/imunologia , Plantas Geneticamente Modificadas/genética , Interferência de RNA
2.
RNA ; 20(12): 1987-99, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25344399

RESUMO

The experimental induction of RNA silencing in plants often involves expression of transgenes encoding inverted repeat (IR) sequences to produce abundant dsRNAs that are processed into small RNAs (sRNAs). These sRNAs are key mediators of post-transcriptional gene silencing (PTGS) and determine its specificity. Despite its application in agriculture and broad utility in plant research, the mechanism of IR-PTGS is incompletely understood. We generated four sets of 60 Arabidopsis plants, each containing IR transgenes expressing different configurations of uidA and CHALCONE Synthase (At-CHS) gene fragments. Levels of PTGS were found to depend on the orientation and position of the fragment in the IR construct. Deep sequencing and mapping of sRNAs to corresponding transgene-derived and endogenous transcripts identified distinctive patterns of differential sRNA accumulation that revealed similarities among sRNAs associated with IR-PTGS and endogenous sRNAs linked to uncapped mRNA decay. Detailed analyses of poly-A cleavage products from At-CHS mRNA confirmed this hypothesis. We also found unexpected associations between sRNA accumulation and the presence of predicted open reading frames in the trigger sequence. In addition, strong IR-PTGS affected the prevalence of endogenous sRNAs, which has implications for the use of PTGS for experimental or applied purposes.


Assuntos
Inativação Gênica , Interferência de RNA , RNA Mensageiro/genética , RNA Interferente Pequeno/genética , Aciltransferases/genética , Arabidopsis/genética , Regulação da Expressão Gênica de Plantas , Sequenciamento de Nucleotídeos em Larga Escala , Sequências Repetidas Invertidas/genética , Plantas Geneticamente Modificadas/genética , Estabilidade de RNA/genética , RNA de Cadeia Dupla/genética , Transdução de Sinais
3.
Plant Physiol ; 160(4): 2125-36, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23060368

RESUMO

The soybean (Glycine max) genome contains 18 members of the 14-3-3 protein family, but little is known about their association with specific phenotypes. Here, we report that the Glyma0529080 Soybean G-box Factor 14-3-3c (SGF14c) and Glyma08g12220 (SGF14l) genes, encoding 14-3-3 proteins, appear to play essential roles in soybean nodulation. Quantitative reverse transcription-polymerase chain reaction and western-immunoblot analyses showed that SGF14c mRNA and protein levels were specifically increased in abundance in nodulated soybean roots 10, 12, 16, and 20 d after inoculation with Bradyrhizobium japonicum. To investigate the role of SGF14c during soybean nodulation, RNA interference was employed to silence SGF14c expression in soybean roots using Agrobacterium rhizogenes-mediated root transformation. Due to the paleopolyploid nature of soybean, designing a specific RNA interference sequence that exclusively targeted SGF14c was not possible. Therefore, two highly similar paralogs (SGF14c and SGF14l) that have been shown to function as dimers were silenced. Transcriptomic and proteomic analyses showed that mRNA and protein levels were significantly reduced in the SGF14c/SGF14l-silenced roots, and these roots exhibited reduced numbers of mature nodules. In addition, SGF14c/SGF14l-silenced roots contained large numbers of arrested nodule primordia following B. japonicum inoculation. Transmission electron microscopy further revealed that the host cytoplasm and membranes, except the symbiosome membrane, were severely degraded in the failed nodules. Altogether, transcriptomic, proteomic, and cytological data suggest a critical role of one or both of these 14-3-3 proteins in early development stages of soybean nodules.


Assuntos
Proteínas 14-3-3/metabolismo , Glycine max/fisiologia , Proteínas de Plantas/metabolismo , Nodulação/fisiologia , Proteínas 14-3-3/genética , Bradyrhizobium/fisiologia , Regulação da Expressão Gênica de Plantas , Inativação Gênica , Genes de Plantas/genética , Immunoblotting , Espectrometria de Massas , Família Multigênica , Filogenia , Proteínas de Plantas/genética , Nodulação/genética , Plantas Geneticamente Modificadas , Proteômica , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Nódulos Radiculares de Plantas/citologia , Nódulos Radiculares de Plantas/genética , Nódulos Radiculares de Plantas/microbiologia , Nódulos Radiculares de Plantas/ultraestrutura , Glycine max/genética , Glycine max/microbiologia , Glycine max/ultraestrutura , Simbiose/genética , Transcriptoma/genética
4.
PLoS One ; 6(8): e24230, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21909385

RESUMO

BACKGROUND: The wheat stripe rust fungus (Puccinia striiformis f. sp. tritici, PST) is responsible for significant yield losses in wheat production worldwide. In spite of its economic importance, the PST genomic sequence is not currently available. Fortunately Next Generation Sequencing (NGS) has radically improved sequencing speed and efficiency with a great reduction in costs compared to traditional sequencing technologies. We used Illumina sequencing to rapidly access the genomic sequence of the highly virulent PST race 130 (PST-130). METHODOLOGY/PRINCIPAL FINDINGS: We obtained nearly 80 million high quality paired-end reads (>50x coverage) that were assembled into 29,178 contigs (64.8 Mb), which provide an estimated coverage of at least 88% of the PST genes and are available through GenBank. Extensive micro-synteny with the Puccinia graminis f. sp. tritici (PGTG) genome and high sequence similarity with annotated PGTG genes support the quality of the PST-130 contigs. We characterized the transposable elements present in the PST-130 contigs and using an ab initio gene prediction program we identified and tentatively annotated 22,815 putative coding sequences. We provide examples on the use of comparative approaches to improve gene annotation for both PST and PGTG and to identify candidate effectors. Finally, the assembled contigs provided an inventory of PST repetitive elements, which were annotated and deposited in Repbase. CONCLUSIONS/SIGNIFICANCE: The assembly of the PST-130 genome and the predicted proteins provide useful resources to rapidly identify and clone PST genes and their regulatory regions. Although the automatic gene prediction has limitations, we show that a comparative genomics approach using multiple rust species can greatly improve the quality of gene annotation in these species. The PST-130 sequence will also be useful for comparative studies within PST as more races are sequenced. This study illustrates the power of NGS for rapid and efficient access to genomic sequence in non-model organisms.


Assuntos
Basidiomycota/genética , Genoma Fúngico/genética , Doenças das Plantas/microbiologia , Análise de Sequência de DNA/métodos , Triticum/microbiologia , Mapeamento de Sequências Contíguas , Elementos de DNA Transponíveis/genética , DNA Fúngico/genética , Ergosterol/biossíntese , Proteínas Fúngicas/metabolismo , Genes Fúngicos/genética , Dados de Sequência Molecular , Sequências Repetitivas de Ácido Nucleico/genética , Sintenia/genética
5.
Mol Plant Microbe Interact ; 24(9): 1051-60, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21815830

RESUMO

The symbiotic interaction between legumes and soil bacteria (e.g., soybean [Glycine max L.] and Bradyrhizobium japonicum]) leads to the development of a new root organ, the nodule, where bacteria differentiate into bacteroids that fix atmospheric nitrogen for assimilation by the plant host. In exchange, the host plant provides a steady carbon supply to the bacteroids. This carbon can be stored within the bacteroids in the form of poly-3-hydroxybutyrate granules. The formation of this symbiosis requires communication between both partners to regulate the balance between nitrogen fixation and carbon utilization. In the present study, we describe the soybean gene GmNMNa that is specifically expressed during the infection of soybean cells by B. japonicum. GmNMNa encodes a protein of unknown function. The GmNMNa protein was localized to the nucleolus and also to the mitochondria. Silencing of GmNMNa expression resulted in reduced nodulation, a reduction in the number of bacteroids per infected cell in the nodule, and a clear reduction in the accumulation of poly-3-hydroxybutyrate in the bacteroids. Our results highlight the role of the soybean GmNMNa gene in regulating symbiotic bacterial infection, potentially through the regulation of the accumulation of carbon reserves.


Assuntos
Bradyrhizobium/fisiologia , Glycine max/fisiologia , Hidroxibutiratos/metabolismo , Nodulação/fisiologia , Poliésteres/metabolismo , Proteínas de Soja/metabolismo , Bradyrhizobium/ultraestrutura , Carbono/metabolismo , DNA de Plantas/genética , Regulação da Expressão Gênica de Plantas , Genes Reporter , Proteínas Mitocondriais/genética , Proteínas Mitocondriais/metabolismo , Nitrogênio/metabolismo , Fixação de Nitrogênio/fisiologia , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/microbiologia , Raízes de Plantas/fisiologia , Raízes de Plantas/ultraestrutura , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/microbiologia , Plantas Geneticamente Modificadas/fisiologia , Plantas Geneticamente Modificadas/ultraestrutura , Interferência de RNA , RNA de Plantas/genética , Proteínas de Soja/genética , Glycine max/genética , Glycine max/microbiologia , Glycine max/ultraestrutura , Simbiose/genética , Simbiose/fisiologia , Nicotiana/genética , Nicotiana/metabolismo , Nicotiana/ultraestrutura
6.
Plant J ; 62(5): 852-64, 2010 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-20230508

RESUMO

A soybean homolog of the tomato FW2.2 gene, here named GmFWL1 (Glycine max FW2.2-like 1), was found to respond strongly to inoculation with the nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum. In tomato, the FW2.2 gene is hypothesized to control 30% of the variance in fruit weight by negatively regulating cell division. In the present study, the induction of GmFWL1 expression in root hair cells and nodules in response to B. japonicum inoculation was documented using quantitative RT-PCR and transcriptional fusions to both beta-glucuronidase (GUS) and green fluorescent protein (GFP). RNAi-mediated silencing of GmFWL1 expression resulted in a significant reduction in nodule number, with a concomitant reduction in nuclear size and changes in chromatin structure. The reduction in nuclear size is probably due to a change in DNA heterochromatinization, as the ploidy level of wild-type and RNAi-silenced nodule cells was similar. GmFWL1 was localized to the plasma membrane. The data suggest that GmFWL1 probably acts indirectly, perhaps through a cellular cascade, to affect chromatin structure/nuclei architecture. As previously proposed in tomato, this function may be a result of effects on plant cell division.


Assuntos
Glycine max/genética , Proteínas de Plantas/metabolismo , Nódulos Radiculares de Plantas/crescimento & desenvolvimento , Bradyrhizobium/fisiologia , Clonagem Molecular , Genes de Plantas , Heterocromatina/metabolismo , Família Multigênica , Filogenia , Proteínas de Plantas/genética , Interferência de RNA , RNA de Plantas/genética , Alinhamento de Sequência , Glycine max/metabolismo
7.
New Phytol ; 184(2): 399-411, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19659660

RESUMO

* Here, nitrogen (N) uptake and metabolism, and related gene expression, were analyzed in germinating spores of Glomus intraradices to examine the mechanisms and the regulation of N handling during presymbiotic growth. * The uptake and incorporation of organic and inorganic N sources into free amino acids were analyzed using stable and radioactive isotope labeling followed by high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS) and liquid scintillation counting and the fungal gene expression was measured by quantitative polymerase chain reaction (Q-PCR). * Quiescent spores store Asp, Ala and Arg and can use these internal N resources during germination. Although not required for presymbiotic growth, exogenous N can also be utilized for the de novo biosynthesis of amino acids. Ammonium and urea are more rapidly assimilated than nitrate and amino acids. Root exudates do not stimulate the uptake and utilization of exogenous ammonium, but the expression of genes encoding a putative glutamate dehydrogenase (GDH), a urease accessory protein (UAP) and an ornithine aminotransferase (OAT) were stimulated by root exudates. The transcript levels of an ammonium transporter (AMT) and a glutamine synthetase (GS) were not affected. * Germinating spores can make effective use of different N sources and the ability to synthesize amino acids does not limit presymbiotic growth of arbuscular mycorrhizal (AM) spores.


Assuntos
Aminoácidos/biossíntese , Genes Fúngicos , Glomeromycota/metabolismo , Micorrizas/metabolismo , Nitrogênio/metabolismo , Esporos Fúngicos/metabolismo , Transporte Biológico , Cromatografia Gasosa-Espectrometria de Massas , Regulação Fúngica da Expressão Gênica , Glomeromycota/genética , Glomeromycota/crescimento & desenvolvimento , Glutamato Desidrogenase/genética , Glutamato Desidrogenase/metabolismo , Micorrizas/crescimento & desenvolvimento , Nitratos/metabolismo , Ornitina-Oxo-Ácido Transaminase/genética , Ornitina-Oxo-Ácido Transaminase/metabolismo , Exsudatos de Plantas/fisiologia , Raízes de Plantas , Compostos de Amônio Quaternário/metabolismo , Esporos Fúngicos/genética , Esporos Fúngicos/crescimento & desenvolvimento , Ureia/metabolismo
8.
Plant Physiol ; 150(1): 521-30, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19279195

RESUMO

Glutathione S-transferases (GSTs) are ubiquitous enzymes that catalyze the conjugation of toxic xenobiotics and oxidatively produced compounds to reduced glutathione, which facilitates their metabolism, sequestration, or removal. We report here that soybean (Glycine max) root nodules contain at least 14 forms of GST, with GST9 being most prevalent, as measured by both real-time reverse transcription-polymerase chain reaction and identification of peptides in glutathione-affinity purified extracts. GST8 was prevalent in stems and uninfected roots, whereas GST2/10 prevailed in leaves. Purified, recombinant GSTs were shown to have wide-ranging kinetic properties, suggesting that the suite of GSTs could provide physiological flexibility to deal with numerous stresses. Levels of GST9 increased with aging, suggesting a role related to senescence. RNA interference studies of nodules on composite plants showed that a down-regulation of GST9 led to a decrease in nitrogenase (acetylene reduction) activity and an increase in oxidatively damaged proteins. These findings indicate that GSTs are abundant in nodules and likely function to provide antioxidant defenses that are critical to support nitrogen fixation.


Assuntos
Glutationa Transferase/fisiologia , Glycine max/enzimologia , Proteínas de Plantas/fisiologia , Nódulos Radiculares de Plantas/enzimologia , Acetileno/metabolismo , Regulação para Baixo , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Cinética , Nitrogenase/metabolismo , Oxirredução , Proteínas de Plantas/genética , Interferência de RNA , Nódulos Radiculares de Plantas/fisiologia , Glycine max/fisiologia
9.
Plant Physiol ; 149(2): 994-1004, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19036836

RESUMO

Apyrases are non-energy-coupled nucleotide phosphohydrolases that hydrolyze nucleoside triphosphates and nucleoside diphosphates to nucleoside monophosphates and orthophosphates. GS52, a soybean (Glycine soja) ecto-apyrase, was previously shown to be induced very early in response to inoculation with the symbiotic bacterium Bradyrhizobium japonicum. Overexpression of the GS52 ecto-apyrase in Lotus japonicus increased the level of rhizobial infection and enhanced nodulation. These data suggest a critical role for the GS52 ecto-apyrase during nodulation. To further investigate the role of GS52 during nodulation, we used RNA interference to silence GS52 expression in soybean (Glycine max) roots using Agrobacterium rhizogenes-mediated root transformation. Transcript levels of GS52 were significantly reduced in GS52 silenced roots and these roots exhibited reduced numbers of mature nodules. Development of the nodule primordium and subsequent nodule maturation was significantly suppressed in GS52 silenced roots. Transmission electron micrographs of GS52 silenced root nodules showed that early senescence and infected cortical cells were devoid of symbiosome-containing bacteroids. Application of exogenous adenosine diphosphate to silenced GS52 roots restored nodule development. Restored nodules contained bacteroids, thus indicating that extracellular adenosine diphosphate is important during nodulation. These results clearly suggest that GS52 ecto-apyrase catalytic activity is critical for the early B. japonicum infection process, initiation of nodule primordium development, and subsequent nodule organogenesis in soybean.


Assuntos
Apirase/metabolismo , Glycine max/enzimologia , Glycine max/genética , Proteínas de Plantas/metabolismo , Pirofosfatases/genética , Pirofosfatases/metabolismo , Difosfato de Adenosina/metabolismo , Sequência de Aminoácidos , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Inativação Gênica , Dados de Sequência Molecular , Nucleotídeos/metabolismo , Pisum sativum/enzimologia , Raízes de Plantas/enzimologia , Raízes de Plantas/genética , Interferência de RNA , RNA Mensageiro/genética , Solanum tuberosum/enzimologia , Transcrição Gênica
10.
New Phytol ; 180(3): 684-695, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18694446

RESUMO

* Root exudates play a key role during the presymbiotic growth phase and have been shown to stimulate hyphal branching and the catabolic metabolism of arbuscular mycorrhizal (AM) fungal spores. * Here, the effect of root exudates on presymbiotic growth, uptake of exogenous carbon and transcript levels for genes putatively involved in the carbon metabolism of germinating spores were determined. * Crude root exudates led to a slight acceleration of spore germination, increased germ tube branching and stimulated uptake and catabolic metabolism of acetate, and to a greater extent of glucose, but had no effect on gene expression. By contrast, partially purified root exudates increased the transcript levels of acyl-CoA dehydrogenase (ss-oxidation of fatty acids to acetyl-CoA), malate synthase (glyoxylate cycle) and glutamine-fructose-6-phosphate aminotransferase (chitin biosynthesis), but did not differ from crude root exudates in their effect on substrate uptake and respiration. The expression of glycogen synthase (glycogen biosynthesis), glucose-6-phosphate dehydrogenase (pentose phosphate pathway) and neutral trehalase (hydrolysis of trehalose) were only marginally or not affected by root exudates. * Root exudates have an effect on both membrane activity and gene expression and the results are discussed in relation to the catabolic and anabolic metabolism of spores during presymbiotic growth.


Assuntos
Carbono/metabolismo , Glomeromycota/metabolismo , Micorrizas/fisiologia , Exsudatos de Plantas/metabolismo , Raízes de Plantas/metabolismo , Esporos Fúngicos/metabolismo , Absorção , Acetatos/metabolismo , Carbono/farmacocinética , Respiração Celular , Expressão Gênica/efeitos dos fármacos , Regulação Fúngica da Expressão Gênica/efeitos dos fármacos , Genes Fúngicos , Germinação , Glomeromycota/genética , Glomeromycota/crescimento & desenvolvimento , Esporos Fúngicos/genética , Esporos Fúngicos/crescimento & desenvolvimento , Simbiose
11.
Mol Plant Microbe Interact ; 21(8): 1027-35, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18616399

RESUMO

The efficiency of beta-glucuronidase (GUS) expression was evaluated with five viral promoters to identify the most suitable promoter or promoters for use in soybean hairy roots, including applications to study the symbiotic interaction with Bradyrhizobium japonicum. Levels of GUS activity were fluorimetrically and histochemically assayed when the GUS (uidA) gene was driven by the Cauliflower mosaic virus (CaMV) 35S promoter and enhanced 35S (E35S) promoter, the Cassava vein mosaic virus (CsVMV) promoter, the Figwort mosaic virus (FMV) promoter, and the Strawberry vein banding virus (SVBV2) promoter. We demonstrate that GUS activity was highest when driven by the FMV promoter and that the promoter activity of 35S and SVBV2 was significantly lower than that of the CsVMV and E35S promoters when tested in soybean hairy roots. In mature soybean root nodules, strong GUS activity was evident when the FMV, 35S, and CsVMV promoters were used. These results indicate that the FMV promoter facilitates the strong expression of target genes in soybean hairy roots and root nodules.


Assuntos
Regulação da Expressão Gênica de Plantas , Glycine max/genética , Vírus de Plantas/genética , Plantas Geneticamente Modificadas/genética , Regiões Promotoras Genéticas , Nódulos Radiculares de Plantas/genética , Genes Reporter , Vetores Genéticos , Glucuronidase/genética , Plasmídeos , Transformação Genética
12.
Nature ; 435(7043): 819-23, 2005 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-15944705

RESUMO

Most land plants are symbiotic with arbuscular mycorrhizal fungi (AMF), which take up mineral nutrients from the soil and exchange them with plants for photosynthetically fixed carbon. This exchange is a significant factor in global nutrient cycles as well as in the ecology, evolution and physiology of plants. Despite its importance as a nutrient, very little is known about how AMF take up nitrogen and transfer it to their host plants. Here we report the results of stable isotope labelling experiments showing that inorganic nitrogen taken up by the fungus outside the roots is incorporated into amino acids, translocated from the extraradical to the intraradical mycelium as arginine, but transferred to the plant without carbon. Consistent with this mechanism, the genes of primary nitrogen assimilation are preferentially expressed in the extraradical tissues, whereas genes associated with arginine breakdown are more highly expressed in the intraradical mycelium. Strong changes in the expression of these genes in response to nitrogen availability and form also support the operation of this novel metabolic pathway in the arbuscular mycorrhizal symbiosis.


Assuntos
Micorrizas/metabolismo , Nitrogênio/metabolismo , Simbiose , Acetatos/metabolismo , Aminoácidos , Arginina/metabolismo , DNA Bacteriano/genética , Daucus carota/genética , Daucus carota/metabolismo , Daucus carota/microbiologia , Regulação da Expressão Gênica , Genes Fúngicos/genética , Genes de Plantas/genética , Dados de Sequência Molecular , Micélio/metabolismo , Micorrizas/genética , Nitratos/metabolismo , Simbiose/genética
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