1.
Biotechniques
; 19(2): 286-90, 1995 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8527151
RESUMO
This article describes the optimization of the hybridization signal obtained with biotinylated oligonucleotides. Optimal number and positions of biotin moieties on a 33-base oligonucleotide probe were determined. The quality of avidin-peroxidase conjugate and the choice of chromogenic substrate influenced detection sensitivity. A signal amplification method was also developed for avidin enzymatic conjugates. These improvements allowed the detection of less than 0.02 fmol of target DNA.