Assuntos
Meios de Contraste , Extravasamento de Materiais Terapêuticos e Diagnósticos/diagnóstico por imagem , Migração de Corpo Estranho/diagnóstico por imagem , Iohexol/análogos & derivados , Mediastino/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Dispositivos de Acesso Vascular , Veia Cava Superior/diagnóstico por imagem , Veia Cava Superior/lesões , Meios de Contraste/farmacocinética , Feminino , Humanos , Iohexol/farmacocinética , Pessoa de Meia-Idade , Derrame Pleural/diagnóstico por imagemRESUMO
The preparation Solutio Betadini propanolica 3% w/w, used as a pre-operative skin disinfectant was examined for its germicidal activity and its chemical stability. The additive activity of iodine and propanol resulted in a wide range of antimicrobial activity against bacteria, including bacterial spores, and fungi, with a rapid onset and sustained duration of action. The solution is easily manufactured and analyzed, its stability is guaranteed for 6 months. Because of its staining property, there is no need of coloured additives to mark the disinfected area. In addition, no skin irritations by the disinfectant were observed so far. Thus, the preparation fulfills the essential requirements for preoperative skin disinfectants and is suitable for the preparation and the compounding in hospital pharmacies.
Assuntos
Bactérias/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Iodo/normas , Iodóforos/normas , Povidona-Iodo/normas , Povidona/análogos & derivados , Estabilidade de Medicamentos , Enterococcus faecalis/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Humanos , Iodóforos/farmacologia , Povidona-Iodo/farmacologia , Proteus mirabilis/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacosRESUMO
20 hly plasmids of different origin and source and 8 recombinant plasmids containing either chromosomally or plasmid encoded hly determinants were characterized with emphasis on their haemolytic activity. Remarkable differences among the hly genes tested were found in their haemolytic activity and in the regulation of the haemolytic activity by the extracellular iron concentration. Under iron-limiting growth conditions the haemolysin secretion encoded by 6 hly plasmids, but not by the remaining 14 hly plasmids and the chromosomal hly genes, was significantly induced. The 6 plasmids all derived from human isolates, belonged to the incompatibility groups FIV and FVI and showed similar restriction patterns. Two hly determinants, encoding an inducible haemolysin secretion, cloned from plasmids isolated in Berne and Paris, respectively, showed identical restriction maps, indicating a common origin. Compared to the hly determinant of a plasmid encoding a noninducible haemolysin secretion, distinct differences, mainly in the flanking regions, were observed.
Assuntos
Cromossomos Bacterianos , Escherichia coli/genética , Genes Bacterianos , Genes , Proteínas Hemolisinas/genética , Plasmídeos , Animais , Enzimas de Restrição do DNA , Escherichia coli/isolamento & purificação , Escherichia coli/patogenicidade , Hemólise , Humanos , Mapeamento de NucleotídeosRESUMO
The haemolytic activity encoded by thirteen hly-plasmids of different origin and sources was examined as a function of the Fe3+-concentration in E. coli fur+ and E. coli fur- strains, respectively. In E. coli fur+ the relatively low haemolytic activity of five hly-plasmids isolated in Berne and one isolated in Paris was increased significantly under iron-limiting growth conditions. Contrastingly, in E. coli fur- strains containing the same plasmids, a considerably higher amount of secreted haemolysin was detected. This activity could not be further increased by limiting the extracellular iron concentration. Seven other hly-plasmids expressed similar and non-inducible amounts of secreted haemolysin in both E. coli fur+ and E. coli fur- strains. These results indicate that the extracellular haemolytic activity encoded by certain hly-plasmids was controlled by the chromosomally encoded fur gene.
