The paradox of expert judgment in rivers ecological monitoring.

A great investment has been done in the last decades in the development of numerical and qualitative assessment methods to classify the ecological quality of water bodies. Yet, in spite of all attempts to avoid subjectivity, expert judgment is still used at numerous steps of the ecological classification and is considered by some authors as indispensible for management purposes. Thus, the aim of this study is to test the hypothesis that expert judgment, when done by the adequate experts (limnologists/river ecologist) with experience in the study area (i.e., natural conditions and expected communities), could be as good as quantitative indices and measures (i.e., result in the same classification), but quicker and with lower cost. For that we compared the classifications (on 13 aspects of rivers ecosystems) attributed by two experts to 20 sites (10 each) located in their study areas, with the classifications of ecological quality based on biological indices (for invertebrates and diatoms), hydromorphology and water chemistry, calculated by an independent team. Our results show that assessments made by experts and those calculated through indices (biological quality and hydromorphology) are globally very similar (RELATE test; Rho = 0.442; p < 0.001, 999 permutations). Most differences were of one class and experts tended to attribute a better condition than indices to the best quality sites but a worse condition to the worse quality sites. A Principal Components Analysis revealed that sites to which experts attributed a moderate quality had higher nitrate concentration and pH but were well oxygenated. The sites classified as poor and bad where those with stronger modifications in their habitats (given by the higher values of HMS). The difference between experts and indices is small but still represents 15% of sites, and includes both situations: the experts or the indices lead to the need of measures (i.e., classifications below class Good). Experts' evaluations on hydromorphological conditions of the channel and margins are also significantly correlated with the quality assessments made by the field team that has no experience in the study area (Rh0 = 0.518; p = 0.001; 999 permutation), indicating geographic independence in the expert judgment. We concluded that expert judgment could be used in the determination of streams and rivers ecological quality, saving money and time and helping to redirect monitoring funds to actual implementation of restoration measures. Yet, classification' scoring methods may still be useful for a better targeting of restoration measures.

Human disturbance affects the long-term spatial synchrony of freshwater invertebrate communities.

Taxonomic and trait composition of invertebrate communities were investigated for 4 reference and 5 disturbed Mediterranean stream sites over a 18 y period, to test the hypotheses that: 1) human disturbance reduces the resilience of communities to extreme climatic events by affecting their functional structure; 2) and as a result, extreme climatic events could lead to asynchronous responses of communities from streams with similar environmental characteristics but differently affected by human disturbance. In reference sites, trait compositions changed differently (were less similar over time) in response to extreme events and variation was less synchronic than taxonomic compositions in the same sites. Oppositely, in disturbed sites there was a marked synchrony in trait composition indicating a functional homogenization in response to human pressures. This limitation in "functional diversity" may involve a lower ability to respond to climatic extreme events and also a reduced potential in ecosystem services.

Do climate and soil influence phenotypic variability in leaf litter, microbial decomposition and shredder consumption?

We tested the hypothesis that water stress and soil nutrient availability drive leaf-litter quality for decomposers and detritivores by relating chemical and physical leaf-litter properties and decomposability of Alnus glutinosa and Quercus robur, sampled together with edaphic parameters, across wide European climatic gradients. By regressing principal components analysis of leaf traits [N, P, condensed tannins, lignin, specific leaf area (SLA)] against environmental and soil parameters, we found that: (1) In Q. robur the condensed tannin and lignin contents increased and SLA decreased with precipitation, annual range of temperature, and soil N content, whereas leaf P increased with soil P and temperature; (2) In A. glutinosa leaves N, P, and SLA decreased and condensed tannins increased with temperature, annual range of temperature, and decreasing soil P. On the other hand, leaf P and condensed tannins increased and SLA decreased with minimum annual precipitation and towards sites with low temperature. We selected contrasting leaves in terms of quality to test decomposition and invertebrate consumption. There were intraspecific differences in microbial decomposition rates (field, Q. robur) and consumption by shredders (laboratory, A. glutinosa). We conclude that decomposition rates across ecosystems could be partially governed by climate and soil properties, affecting litter quality and therefore decomposers and detritivores. Under scenarios of global warming and increased nutrients, these results suggest we can expect species-specific changes in leaf-litter properties most likely resulting in slow decomposition with increased variance in temperatures and accelerated decomposition with P increase.

Importance of fungi in the diet of Gammarus pulex and Asellus aquaticus : II. Effects on growth, reproduction and physiology.

An important component of the interaction between macroinvertebrates and leaf litter in streams in the extent to which consumers can differentiate between undecomposed and decomposing leaves. The detritivores Gammarus pulex and Asellus aquaticus fed preferentially on conditioned rather on unconditioned leaf material. Growth in A. aquaticus was significantly reduced when unconditioned leaves were provided, but in G. pulex no significant effect of conditioning on growth was observed. The capacity of G. pulex to tolerate reductions in food quality seems to be a consequence of a compensatory system in which respiration rates change to compensate for reductions in food quality. In this way a constant growth rate is maintained. Increases in ingestion rates to compensate for low quality food were not observed.

Importance of fungi in the diet of Gammarus pulex and Asellus aquaticus I: feeding strategies.

The importance of fungi in the trophic biology of the freshwater detritivores Gammarus pulex and Asellus aquaticus was investigated. Inspection of leaves used in feeding trials indicated that whereas A. aquaticus scrapes at the leaf surface, G. pulex bites through the leaf material. Both species discriminated between fungal mycelia, fungally colonized and uncolonized leaf material but, although A. aquaticus selectively consumed fungal mycelia, G. pulex fed preferentially on leaf material. Fungi appear to be an important food source for A. aquaticus and selection of food material was positively correlated with fungal biomass. In contrast, for G. pulex, fungi appear to be more important as modifiers of leaf material. However, no significant correlations were found between food preference and any of the leaf modifications measured.