RESUMO
El Síndrome Burnout se caracteriza por agotamiento emocional, despersonalización y baja realización personal, que suele producirse en entornos de estrés laboral continuo, afectando gradualmente a trabajadores cuya labor les exige implicación emocional con personas. La calidad de vida profesional es la calidad que uno siente con relación a su trabajo. Este trabajo, transversal analítico, tuvo como objetivo determinar la frecuencia del Síndrome Burnout y de deficiencias en la calidad de vida laboral del personal de enfermería de los servicios de hospitalización de Medicina Interna del Hospital Roosevelt. Asimismo, se determinó qué relación presentaban las subescalas del inventario Maslach de Síndrome de Burnout y las subescalas de calidad de vida profesional del instrumento ProQOL. Se incluyó a 55 trabajadores de enfermería de los servicios de Medicina Interna del Hospital Roosevelt, durante 2016. Se observó una frecuencia de Síndrome de Burnout de 3.6 %, agotamiento emocional alto de 20 %, despersonalización de 24.5 % y baja satisfacción laboral de 30 %. No se encontró ningún caso de calidad de vida laboral deficiente. Se observó asociación significativa entre satisfacción laboral y realización personal (Tau = .36; p = .014), burnout con realización personal (Tau = -.31; p = .040) y estrés traumático con despersonalización (Tau = .36; p = .014). Se concluyó que algunos dominios de la escala de calidad de vida laboral poseen alguna relación de débil a moderada con los dominios de la escala de Burnout
Burnout Syndrome is characterized by emotional exhaustion, depersonalization and low personal fulfillment, which usually occurs in environments of continuous work stress, gradually affecting workers whose work requires emotional involvement with people. The quality of professional life is the quality one feels in relation to their work. This work, cross-sectional analysis, aimed to determine the frequency of Burnout Syndrome and deficiencies in the quality of working life of the nursing staff of the Internal Medicine hospitalization services of the Roosevelt Hospital. Likewise, it was determined what relation the subscales of the Maslach inventory of Burnout Syndrome and the subscales of quality of professional life of the ProQOL instrument presented. Fifty-five nursing workers from the Internal Medicine departments of Roosevelt Hospital were included during 2016. A frequency of Burnout Syndrome of 3.6%, high emotional exhaustion of 20%, depersonalization of 24.5% and low job satisfaction of 30% was observed. No case of poor quality of working life was found. There was a significant association between job satisfaction and personal fulfillment (Tau = .36; p = .014), burnout with personal fulfillment (Tau = -.31; p = .040) and traumatic stress with depersonalization (Tau = .36; p = .014). It was concluded that some domains of the quality of work life scale have some weak to moderate relationship with the domains of the Burnout scale
RESUMO
OBJECTIVES: The present study aimed at developing tissue-engineered mitral valves based on cell-free ovine mitral allografts. METHODS: The ovine mitral valves (OMVs) (n = 46) were harvested in the local slaughter house. They were decellularized using detergent solutions and DNase. The effectiveness of decellularization was assessed by histological (haematoxylin-eosin, Movat's pentachrome) and immunofluorescent staining (for DNA and α-Gal), and DNA-quantification. To reveal the receptiveness of decellularized tissue to endothelial cells (ECs), the valve leaflets were reseeded with ovine ECs, derived from endothelial progenitor cells in vitro. For assessment of biomechanical properties, uniaxial tensile tests were carried out. RESULTS: Histology and immunofluorescent staining revealed absence of cell nuclei in decellularized leaflets, chordae and papillary muscles. According to the software for immunofluorescence analysis, reduction in DNA and α-Gal was 99.9 and 99.6%, respectively. DNA-quantification showed 71.2% reduction in DNA content without DNase and 96.4% reduction after DNase treatment. Decellularized leaflets were comparable with native in ultimate tensile strain (native, 0.34 ± 0.09 mm/mm, vs decellularized, 0.44 ± 0.1 mm/mm; P = 0.09), and elastin modulus (native, 0.39 ± 0.27, vs decellularized, 0.57 ± 0.55, P = 0.46), had increased ultimate tensile stress (native, 1.23 ± 0.35 MPa, vs decellularized 2.12 ± 0.43 MPa; P = 0.001) and collagen modulus (native, 5.5 ± 1.26, vs decellularized, 8.29 ± 2.9; P = 0.04). After EC seeding, immunofluorescent staining revealed a monolayer of CD31-, eNOS- and vWF-positive cells on the surface of the leaflet, as well as a typical cobble-stone morphology of those cells. CONCLUSIONS: Decellularization of ovine mitral valve results in a mitral valves scaffold with mechanical properties comparable with native tissue, and a graft surface, which can be repopulated by endothelial cells.
