RESUMO
This review concerns structural and functional properties of human vascular endothelial growth factor as well as the process of alternative splicing of its transcript. Regulation of VEGF gene transcription and participation of the protein in the angiogenesis of tumours are also broadly discussed.
Assuntos
Fatores de Crescimento Endotelial/genética , Linfocinas/genética , Neoplasias/fisiopatologia , Neovascularização Patológica/genética , Animais , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Transcrição Gênica , Fator A de Crescimento do Endotélio Vascular , Fatores de Crescimento do Endotélio VascularRESUMO
Proliferative and angiogenic activity of tissue specimens taken from women with various vulvar pathologies were evaluated by determining the number of mRNA VEGF molecules and H4 histone mRNA molecules, by means of the QRT-PCR (TaqMan) technique. Following a cluster analysis the results, where normalised. Euclidean distances were used, all the cases were classified into three groups of pathologies. Group I included low degree vulvar pathologies, group II included high degree vulvar pathologies and group III included vulvar pathologies with high proliferative and angiogenic activity. Significant differences were found in the proliferative and angiogenic activity between groups I and III, and between groups II and III, while no statistically significant differences were found between groups I and II.
Assuntos
Carcinoma in Situ/patologia , Fatores de Crescimento Endotelial/genética , Histonas/genética , Linfocinas/genética , Neovascularização Patológica/patologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Neoplasias Vulvares/patologia , Carcinoma in Situ/fisiopatologia , Divisão Celular , DNA de Neoplasias/análise , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Neovascularização Patológica/fisiopatologia , RNA Mensageiro/análise , Taq Polimerase , Fator A de Crescimento do Endotélio Vascular , Fatores de Crescimento do Endotélio Vascular , Neoplasias Vulvares/fisiopatologiaRESUMO
SUBJECT: The excessive proliferative activity of endometrial cells drives to hyperplasia, via states: simplex hyperplasia, complex and atypical hyperplasia, and finally preneoplastic state may arise. New molecular markers and their ability to differentiate physiological and pathological states are described. DESIGN: The aim of this study was to evaluate the usefulness of histone H3 gene expression level as a marker of hyperplasia of endometrium. MATERIAL AND METHODS: The level of H3 mRNA was estimated using RT-QPCR method in proliferative phase (n = 5) and endometrial hyperplasia simplex (n = 8). Differences in expression level of H3, were evaluated by Student's t-test (t-test for independent samples). RESULTS: In our experiments the average mRNA H3 expression was significantly higher(p < 0.05, p = 0.03) in case of hyperplasia simplex in comparison to H3 expression in proliferative state of endometrium. CONCLUSION: The results confirm advantage of estimation of H3 mRNA level as a marker of cell proliferation in endometrium.
Assuntos
Biomarcadores Tumorais/análise , Hiperplasia Endometrial/metabolismo , Histonas/análise , Lesões Pré-Cancerosas/química , Adulto , Biomarcadores Tumorais/sangue , Hiperplasia Endometrial/sangue , Hiperplasia Endometrial/patologia , Endométrio/química , Endométrio/patologia , Feminino , Regulação Neoplásica da Expressão Gênica , Histonas/sangue , Histonas/genética , Humanos , Lesões Pré-Cancerosas/sangue , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Telomerase is a specialized ribonucleoprotein polymerase that elongates telomeres. To clarify the molecular mechanisms through which telomerase is activated in normal endometrium, we examined the expression profiles of each telomerase subunit in this tissues. A total of 22 normal human endometrium in various menstrual phases were examined for the expression of each telomerase subunit (hTERT, hTR, TP-1) using RT-QPCR. The hTERT as well as TP-1 subunit had higher expression levels in proliferative than secretory phase endometrium. Expression levels of hTR did not change during menstrual cycle. Our data showed the strong correlation between the level of expression of telomerase in human endometrium and phase of menstrual cycle.
