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1.
Front Plant Sci ; 13: 1067096, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36743541

RESUMO

Mountain ecosystems are sensitive to climate fluctuations; however, the scarcity of instrumental data makes necessary the use of complementary information to study the effect of climate change on these systems. Remote sensing permits studying the dynamics of vegetation productivity and wetlands in response to climate variability at different scales. In this study we identified the main climate variables that control vegetation dynamics and water balance in Cumbres Calchaquíes, NW Argentina. For this, we built annual time series from 1986 to 2019 of Soil Adjusted Vegetation Index (SAVI, to quantify spare vegetation productivity), lake area, and snow-ice cover of peatlands, as indicators of mountain productivity and hydrology. We used a decompose function to explore trend, seasonality and random signal of the three-time series, and explored for significant changes in the mean value of consecutive periods. We used correlational analysis to explore their associations with climate records at local, regional, and global scales. The results showed that, SAVI and hydrological indicators presented different fluctuation patterns more pronounced since 2012, when they showed divergent trends with increasing SAVI and decreasing lake area and snow-ice cover. The three indicators responded differently to climate; SAVI increased in warmer years and lake area reflected the water balance of previous years. Snow-ice cover of peatlands was highly correlated with lake area. La Niña had a positive effect on lake area and snow-ice cover and a negative on SAVI, while El Niño had a negative effect on SAVI. Fluctuations of lake areas were synchronized with lake area in the nearby Argentinian puna, suggesting that climate signals have regional extent. The information provided by the three hydroclimate indicators is complementary and reflects different climate components and processes; biological processes (SAVI), physical processes (snow ice cover) and their combination (lake area). This study provides a systematic accessible replicable tool for mountain eco-hydrology long-term monitoring.

2.
Ecol Evol ; 8(23): 11554-11567, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30598756

RESUMO

As global climate change leads to warmer and dryer conditions in the central Andes, alpine plant communities are forced to upward displacements following their climatic niche. Species range shifts are predicted to have major impacts on alpine communities by reshuffling species composition and abundances. Using a standardized protocol, we surveyed alpine plant communities in permanent plots on four high Andean summits in NW Argentina, which range from 4,040 to 4,740 m a.s.l. After a baseline survey in 2006-2008, we resurvey the same plots in 2012, and again in 2017. We found a significant decrease in plant cover, species richness, and diversity across the elevation gradient in the three censuses and a strong decrease in soil temperature along the elevation gradient. We found a high plant community turnover (37%-49%) among censuses, differentiating according to summits and aspects; major changes of community turnover were observed in the lowest summit (49%) and on the northern (47%) and western (46%) aspects. Temporal patterns in community changes were represented by increases in plant cover in the highest summit, in species richness in the lower summit, and in diversity (Shannon index) in the four summits, over time, together with increase in small herbs and non-tussock grasses. We suggest that the observed trend in plant community dynamics responds to short-term temperature and precipitation variability, which is influenced by El Niño Southern Oscillation (ENSO), and due to time lags in plant community response, it may take much longer than one decade for the observed trends to become stables and statistically significant. Our study provides an important foundation for documenting more profound changes in these subtropical alpine plant communities as global climate change continues.

3.
Phytochemistry ; 117: 332-339, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26125943

RESUMO

A 8ß-angeloyloxy-9α-hydroxy-14-oxo-acanthospermolide and five known melampolide sesquiterpene lactones (uvedalin, enhydrin, polymatin B, sonchifolin, and fluctuanin) were isolated from the leaves of Smallanthus sonchifolius. The compounds were identified by 1D-, 2D-NMR, HRMS, IR and UV analyses. In vitro cytotoxicity assays (MTT) showed that these sesquiterpene lactones display poor cytotoxic effects on peripheral blood mononuclear cells (PBMC) of healthy human subjects, whereas a strong cytotoxicity was observed in leukemia and pancreas cancer cells. For the mechanism of action of polymatin B, oxidative stress seems to be involved. Interestingly, reactive oxygen species (ROS) formation mainly induced different effects: apoptosis in CCRF-CEM cells, necroptosis in CEM-ADR5000 cells through induction of RIP1K, neither apoptosis nor necroptosis in MIA-PaCa-2 cells. Additionally, cells also died partly by necrosis.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Asteraceae/química , Lactonas/farmacologia , Sesquiterpenos/farmacologia , Antineoplásicos Fitogênicos/química , Ciclo Celular/efeitos dos fármacos , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos/métodos , Ensaios de Seleção de Medicamentos Antitumorais/métodos , Humanos , Lactonas/química , Leucemia de Células T/tratamento farmacológico , Leucemia de Células T/patologia , Leucócitos Mononucleares/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Estresse Oxidativo/efeitos dos fármacos , Sesquiterpenos/química , Sesquiterpenos de Germacrano/química , Sesquiterpenos de Germacrano/farmacologia
4.
Chem Biol Interact ; 209: 35-47, 2014 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-24309157

RESUMO

The aim of the present study was to analyze the in vivo hypoglycaemic effects of both decoction of Smallanthus macroscyphus leaves and pure crystalline polymatin A isolated from its leaves. Phytochemical analysis of the leaf decoction showed that its major constituents were caffeic, chlorogenic and three dicaffeoilquinic acids, together with the sesquiterpene lactone polymatin A. Oral glucose tolerance test in normal rats was performed to evaluate the hypoglycemic activity and to choose the minimum effective dose of the decoction and polymatin A. They have effective hypoglycemic activity at the minimum dose of 140 mg dry extract and 14 mg crystalline powder/kg body weight, respectively, and were selected for the following experiments. Oral administration of a single-dose of decoction produced a moderate lowering effect in fasting glycemia of normal rats, whereas polymatin A had no significant effect. We also assessed the effect of a single-dose on post-prandial blood glucose, resulting in an inhibition of the hyperglycemic peak after sucrose overload. Daily administration of decoction or polymatin A for 4 weeks produced an effective glycemic control in diabetic animals, with a decrease in urinary glucose excretion and a significant reduction in the HbA1c levels. Although there were no significant increases in plasma insulin levels, both treatments improved the fasting blood glucose/insulin ratio. In vivo acute toxicity studies were performed in adult Wistar rats. There were no deaths or signs of toxicity observed after oral administration of decoction or polymatin A at any dose level up to the highest dose tested (14.0 and 2.8 g/kg, respectively). The results presented here strongly support the notion that S. macroscyphus represents a new source of antidiabetic compounds that could help to manage diabetes more efficiently and safely.


Assuntos
Asteraceae/química , Glicemia/efeitos dos fármacos , Hipoglicemiantes/farmacologia , Lactonas/farmacologia , Extratos Vegetais/farmacologia , Sesquiterpenos/farmacologia , Administração Oral , Animais , Glicemia/análise , Cromatografia Líquida de Alta Pressão , Diabetes Mellitus Experimental/tratamento farmacológico , Relação Dose-Resposta a Droga , Cromatografia Gasosa-Espectrometria de Massas , Hipoglicemiantes/química , Lactonas/química , Masculino , Extratos Vegetais/química , Folhas de Planta/química , Ratos , Ratos Wistar , Sesquiterpenos/química
5.
Nat Prod Res ; 27(10): 916-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-22889184

RESUMO

The anti-inflammatory, antioxidant, antimicrobial and cytotoxic activities of the hexane (HE), chloroform (CE) and methanol (ME) extracts obtained from the bark of Prunus tucumanensis Lillo were investigated. Both ME and CE extracts displayed a significant in vitro anti-inflammatory activity similar to dexamethasone and to a commercial formulation (Pygeum) used for the treatment of benign prostatic hyperplasia (BPH). ME exhibited powerful antioxidant (67.6% relative to BHT) and free radical scavenging (RC50 = 5 ppm) activities, antimicrobial activities against Staphylococcus aureus and Mycobacterium smegmatis and did not show any cytotoxic effect on human-derived macrophage cells. Chemical analyses showed that (2 R,3 R)-3,5,7,3',5'-pentahydroxyflavan, ß-sitosterol and ß-sitosterol-3-O-ß-D-glucopyranoside (daucosterol) are relevant components of ME.


Assuntos
Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Casca de Planta/química , Prunus/química , Compostos de Bifenilo/química , Humanos , Testes de Sensibilidade Microbiana , Picratos/química , Staphylococcus aureus/efeitos dos fármacos
6.
Br J Nutr ; 109(11): 1971-9, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23137694

RESUMO

Beneficial effects of prebiotics like inulin and fructo-oligosaccharides (FOS) have been proven in health and nutrition. Yacon (Smallanthus sonchifolius), an Andean crop, contains FOS (50­70% of its dry weight) and, therefore, is considered a prebiotic. Commercial FOS can upregulate total secretory IgA (S-IgA) in infant mice, prevent infection with Salmonella in swine or enhance immune response for Salmonella vaccine in a mouse model. Previously, we found that administration of yacon root flour regulates gut microbiota balance and has immunomodulatory effects without inflammatory responses. The aim of the present paper is to analyse if yacon prevents enteric infection caused by a strain of Salmonella enteritidis serovar Typhimurium (S. Typhimurium) in a mouse model. BALB/c mice were supplemented with yacon flour (45 d), challenged with S. Typhimurium and killed to study pathogen translocation, total and specific IgA production by ELISA, presence of IgA and other cytokines and Toll-like receptor 4 (TLR4) and clustor of differentiation 206 (CD206) receptors positive cells by immunofluorescence and histological changes. Yacon flour administration had a protective effect from 15 to 30 d of treatment. We found a peak of total S-IgA production without translocation of the pathogen for these periods. At 30 d, there was an increase in IL-6 and macrophage inflammatory proteins-1aþ cells and expression of the receptors CD206 and TLR4. Yacon flour did not have incidence in pathogen-specific S-IgA production. Longer periods (45 d) of administration had no protective effect. Therefore, yacon can prevent enteric infection caused by S. Typhimurium when given up to 30 d; this effect would be mediated by enhancing non-specific immunity, such as total S-IgA, that improves the immunological intestinal barrier.


Assuntos
Asteraceae/química , Oligossacarídeos/farmacologia , Raízes de Plantas/química , Salmonelose Animal/terapia , Salmonella typhimurium , Animais , Líquidos Corporais/química , Líquidos Corporais/metabolismo , Técnica Indireta de Fluorescência para Anticorpo , Imunoglobulina A/química , Imunoglobulina A/metabolismo , Intestino Delgado/metabolismo , Intestino Delgado/microbiologia , Intestino Delgado/patologia , Fígado/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Oligossacarídeos/química , Prebióticos , Salmonelose Animal/patologia , Baço/microbiologia
7.
AoB Plants ; 2011: plr022, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22476492

RESUMO

BACKGROUND AND AIMS: The basic set of adaptations necessary for salinity tolerance in vascular plants remains unknown. Although much has been published on salinity stress, almost all studies deal with spermatophytes. Studies of salinity tolerance in pteridophytes are relatively rare but hold promise for revealing the fundamental adaptations that all salt-tolerant vascular plants may share. The most basal pteridophytes to exhibit salinity tolerance are members of the genus Equisetum, including the giant horsetail, Equisetum giganteum, the only pteridophyte to occur in salinity-affected regions of the Atacama Desert valleys of northern Chile. Here it can constitute a significant vegetation component, forming dense stands of shoots >4 m high. METHODOLOGY: Physiological parameters (stomatal conductances; efficiency of photosystem II; sap osmotic potential) were measured in E. giganteum populations in northern Chile across a range of groundwater salinities at 11 sites. In addition, Na, K, electrical conductivity and total plant water potential were measured in the plants and groundwater from each site. PRINCIPAL RESULTS: Equisetum giganteum exhibits similar stomatal conductances and photochemical efficiencies of photosystem II across a wide range of groundwater salinities. It lowers cell sap osmotic potential with increasing salinity and produces positive root pressure, as evidenced by guttation, at the full range of salinities experienced in the Atacama Desert. Equisetum giganteum maintains low Na concentrations in its xylem fluid and cell sap when soil water Na is high. It also maintains high K/Na ratios in xylem fluid and cell sap when soil water has low K/Na ratios. CONCLUSIONS: Equisetum giganteum is well adapted to salinity stress. Efficient K uptake and Na exclusion are important adaptations and closely similar to those of the facultative halophyte fern Acrostichum aureum.

8.
Chem Biol Interact ; 185(2): 143-52, 2010 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-20211156

RESUMO

The aim of the present study was to determine the in vivo hypoglycemic activity of five organic extracts and enhydrin obtained from yacon leaves. The main constituents of the most active fraction were identified. Five organic extracts and pure crystalline enhydrin were administered to normoglycemic, transiently hyperglycemic and streptozotocin (STZ)-diabetic rats. The fasting and post-prandial blood glucose, and serum insulin levels were estimated and an oral glucose tolerance test (OGTT) was performed for the evaluation of hypoglycemic activity and dose optimization of each extract. We found that the methanol, butanol and chloroform extracts showed effective hypoglycemic activity at minimum doses of 50, 10 and 20mg/kg body weight, respectively, and were selected for further experiments. Oral administration of a single-dose of each extract produced a slight lowering effect in the fasting blood glucose level of normal healthy rats, whereas each extract tempered significantly the hyperglycemic peak after food ingestion. Daily administration of each extract for 8 weeks produced an effective glycemic control in diabetic animals with an increase in the plasma insulin level. Phytochemical analysis of the most active fraction, the butanol extract, showed that caffeic, chlorogenic and three dicaffeoilquinic acids were significant components. Additionally, enhydrin, the major sesquiterpene lactone of yacon leaves, was also effective to reduce post-prandial glucose and useful in the treatment of diabetic animals (minimum dose: 0.8mg/kg body weight). The results presented here strongly support the notion that the phenolic compounds above as well as enhydrin are important hypoglycemic principles of yacon leaves that could ameliorate the diabetic state.


Assuntos
Asteraceae/química , Diabetes Mellitus Experimental/tratamento farmacológico , Hipoglicemiantes/uso terapêutico , Extratos Vegetais/farmacologia , Folhas de Planta/química , Animais , Glicemia/metabolismo , Peso Corporal/efeitos dos fármacos , Peso Corporal/fisiologia , Ácido Clorogênico/química , Ácido Clorogênico/classificação , Ácido Clorogênico/farmacologia , Ácido Clorogênico/uso terapêutico , Diabetes Mellitus Experimental/induzido quimicamente , Diabetes Mellitus Experimental/metabolismo , Relação Dose-Resposta a Droga , Teste de Tolerância a Glucose , Hipoglicemiantes/química , Hipoglicemiantes/farmacologia , Insulina/sangue , Masculino , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Ratos , Ratos Wistar , Sesquiterpenos/química , Sesquiterpenos/farmacologia , Sesquiterpenos/uso terapêutico , Solventes/química , Estreptozocina/toxicidade , Fatores de Tempo
9.
Nat Prod Commun ; 5(11): 1721-6, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21213966

RESUMO

Two new acyclic diterpenoids, smaditerpenic acid E (1a) and F (2a), along with nineteen melampolide-type sesquiterpene lactones, six of them not previously reported in yacon, were isolated from the methylene chloride leaf rinse extract. Their structures were elucidated from 1D and 2D NMR experiments and gas chromatography coupled to mass spectrometry.


Assuntos
Ácidos Acíclicos/química , Asteraceae/química , Diterpenos/química , Folhas de Planta/química , Estrutura Molecular
10.
Clin Nutr ; 28(2): 182-7, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19254816

RESUMO

BACKGROUND & AIMS: Syrup obtained from yacon roots could be well positioned as a nutraceutical product due to its high fructooligosaccharides content. We examined the beneficial effects and tolerance of yacon syrup on human health. METHODS: Obese and slightly dyslipidemic pre-menopausal women were studied over a 120-day period in a double-blind placebo-controlled experiment. We used two doses of yacon syrup, 0.29 g and 0.14 g fructooligosaccharides/kg/day. At the start and end of the study, anthropometric measurements, blood glucose, calcium, lipid and insulin concentrations and Homeostasis Model Assessment index were determined. RESULTS: The recommended daily consumption of yacon syrup with no undesirable gastrointestinal effects is 0.14 g fructooligosaccharides/kg. Daily intake of yacon syrup produced a significant decrease in body weight, waist circumference and body mass index. Additionally, decrease in fasting serum insulin and Homeostasis Model Assessment index was observed. The consumption of yacon syrup increased defecation frequency and satiety sensation. Fasting glucose and serum lipids were not affected by syrup treatment and the only positive effect was found in serum LDL-cholesterol levels. CONCLUSIONS: Yacon syrup is a good source of fructooligosaccharides and its long-term consumption produced beneficial health effects on obese pre-menopausal women with insulin resistance.


Assuntos
Resistência à Insulina , Obesidade/dietoterapia , Extratos Vegetais/administração & dosagem , Preparações de Plantas/farmacologia , Adulto , Antropometria , Glicemia/efeitos dos fármacos , Índice de Massa Corporal , Peso Corporal/efeitos dos fármacos , Cálcio/sangue , Relação Dose-Resposta a Droga , Método Duplo-Cego , Dislipidemias/dietoterapia , Feminino , Humanos , Insulina/sangue , Lipídeos/sangue , Pessoa de Meia-Idade , Resposta de Saciedade/efeitos dos fármacos
11.
Am J Bot ; 96(10): 1839-48, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21622305

RESUMO

Many crops are polyploids, and it can be challenging to untangle the often complicated history of their origins of domestication and origins of polyploidy. To complement other studies of the origins of polyploidy of the octoploid tuber crop oca (Oxalis tuberosa) that used DNA sequence data and phylogenetic methods, we here compared AFLP data for oca with four wild, tuber-bearing Oxalis taxa found in different regions of the central Andes. Results confirmed the divergence of two use-categories of cultivated oca that indigenous farmers use for different purposes, suggesting the possibility that they might have had separate origins of domestication. Despite previous results with nuclear-encoded, chloroplast-expressed glutamine synthetase suggesting that O. picchensis might be a progenitor of oca, AFLP data of this species, as well as different populations of wild, tuber-bearing Oxalis found in Lima Department, Peru, were relatively divergent from O. tuberosa. Results from all analytical methods suggested that the unnamed wild, tuber-bearing Oxalis found in Bolivia and O. chicligastensis in NW Argentina are the best candidates as the genome donors for polyploid O. tuberosa, but the results were somewhat equivocal about which of these two taxa is the more strongly supported as oca's progenitor.

12.
Food Chem Toxicol ; 43(11): 1657-65, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15979774

RESUMO

Yacon roots are a rich source of fructooligosaccharides (FOS) and have a long use tradition as food in the Andean region. However, there are no published reports regarding their toxicology and use safety. The aim of this study was to analyze the effects of subchronic (4-months) oral consumption of dried yacon root flour as a diet supplement using normal Wistar rats. Two daily intake levels were used, equivalent to 340 mg and 6800 mgFOS/body weight, respectively. Yacon administered as a diet supplement was well tolerated and did not produce any negative response, toxicity or adverse nutritional effect at both intake levels used. Yacon root consumption showed no hypoglycemic activity in normal rats and resulted in significantly reduced post-prandial serum triacylglycerol levels in both doses assayed. Conversely, serum cholesterol reduction was not statistically significant. Cecal hypertrophy was observed in rats fed only the high dose. Our results indicating lack of toxicity and a certain beneficial metabolic activity in normal rats warrant further experiments with normal subjects and patients suffering metabolic disorders. They should also be considered when establishing the regulatory framework of this natural product by national health authorities and international trade agencies.


Assuntos
Asteraceae/toxicidade , Suplementos Nutricionais , Animais , Asteraceae/química , Glicemia/metabolismo , Peso Corporal/efeitos dos fármacos , Colesterol/sangue , Carboidratos da Dieta/análise , Suplementos Nutricionais/efeitos adversos , Ingestão de Alimentos , Feminino , Teste de Tolerância a Glucose , Mucosa Intestinal/citologia , Mucosa Intestinal/efeitos dos fármacos , Masculino , Oligossacarídeos/análise , Raízes de Plantas/química , Raízes de Plantas/toxicidade , Ratos , Ratos Wistar , Sobrevida , Triglicerídeos/análise , Urina/química
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