Effects of persistent organic pollutants on telomere dynamics are sex and age-specific in a wild long-lived bird.

Chemical pollution is a major man-made environmental threat to ecosystems and natural animal populations. Of concern are persistent organic pollutants (POPs), which can persist in the environment for many years. While bioaccumulating throughout the lives of wild animals, POPs can affect their health, reproduction, and survival. However, measuring long-term effects of POPs in wild populations is challenging, and therefore appropriate biomarkers are required in wildlife ecotoxicology. One potential target is telomere length, since telomere preservation has been associated to survival and longevity, and stressors as chemical pollution can disrupt its maintenance. Here, we investigated the effects of different classes of POPs on relative telomere length (RTL) and its rate of change (TROC) in wild long-lived Alpine swifts (Tachymarptis melba). As both RTL and TROC are often reported to differ between sexes and with chronological age, we tested for sex- and age-specific (pre-senescent vs. senescent, ≥ 9 age of years, individuals) effects of POPs. Our results showed that senescent females presented longer RTL and elongated telomeres over time compared to pre-senescent females and males. These sex- and age-related differences in RTL and TROC were influenced by POPs, but differently depending on whether they were organochlorine pesticides (OCPs) or industrial polychlorinated biphenyls (PCBs). OCPs (particularly drins) were negatively associated with RTL, with the strongest negative effects being found in senescent females. Conversely, PCBs led to slower rates of telomere shortening, especially in females. Our study indicates diametrically opposed effects of OCPs on RTL and PCBs on TROC, and these effects were more pronounced in females and senescent individuals. The mechanisms behind these effects (e.g., increased oxidative stress by OCPs; upregulation of telomerase activity by PCBs) remain unknown. Our results highlight the importance in wildlife ecotoxicology to account for sex- and age-related effects when investigating the health effects of pollutants on biomarkers such as telomeres.

Is minimally-invasive sampling the future of persistent organic pollutant (POP) research in birds? A meta-analysis on tissue comparisons.

Persistent organic pollutants (POPs) bioaccumulate in the food chain and can cause ecotoxicity. In wild bird populations, various tissues are used to determine POP levels, including invasive (e.g., brain, fat, kidney, liver, muscle) and minimally-invasive tissues (e.g., blood, feather, preen oil). Minimally-invasive sampling, which does not require the death of the animal, opens new prospects for sampling birds as sentinels of environmental pollution and its consequences on fitness. However, POP variability between tissues is understudied, which is an essential prerequisite for making a reasoned choice about which tissues to sample. Here, we performed a meta-analysis of eight tissues across 115 studies comparing tissues across POP groups. We demonstrate increased use of minimally-invasive measures between 1974 and 2020. When grouping tissue correlations into three groups, "invasive:invasive", "invasive:minimally-invasive" and "minimally-invasive:minimally-invasive", we found that all three groups produced moderate to strong positive correlations with no difference seen between comparison groups. We demonstrate (1) lower POP concentrations in preen oil than fat, but no difference in detection frequencies, supporting preen oil use; (2) blood showed high concentration variability dependent on POP group but detection frequencies were comparable to liver and kidney; and (3) feathers demonstrated a significantly lower detection frequency than other matrices measured. By further researching minimally-invasive tissues, we increase our understanding of whether minimally-invasive tissues are ecologically representative of body-level toxicity. Our study supports blood and preen oil as substitutes for invasive measures when sampling living bird populations as they represent internal POP concentrations and provide significant benefits both practically and ethically.

Food insecurity increases energetic efficiency, not food consumption: an exploratory study in European starlings.

Food insecurity-defined as limited or unpredictable access to nutritionally adequate food-is associated with higher body mass in humans and birds. It is widely assumed that food insecurity-induced fattening is caused by increased food consumption, but there is little evidence supporting this in any species. We developed a novel technology for measuring foraging, food intake and body mass in small groups of aviary-housed European starlings (Sturnus vulgaris). Across four exploratory experiments, we demonstrate that birds responded to 1-2 weeks of food insecurity by increasing their body mass despite eating less. Food-insecure birds therefore increased their energetic efficiency, calculated as the body mass maintained per unit of food consumed. Mass gain was greater in birds that were lighter at baseline and in birds that faced greater competition for access to food. Whilst there was variation between experiments in mass gain and food consumption under food insecurity, energetic efficiency always increased. Bomb calorimetry of guano showed reduced energy density under food insecurity, suggesting that the energy assimilated from food increased. Behavioural observations of roosting showed inconsistent evidence for reduced physical activity under food insecurity. Increased energetic efficiency continued for 1-2 weeks after food security was reinstated, indicating an asymmetry in the speed of the response to food insecurity and the recovery from it. Future work to understand the mechanisms underlying food insecurity-induced mass gain should focus on the biological changes mediating increased energetic efficiency rather than increased energy consumption.

Collective learning, change and improvement in health care: trialling a facilitated learning initiative with general practice teams.

RATIONALE, AIMS AND OBJECTIVES: Many patients, families, health care professionals and politicians desire for quality improvement within the UK National Health Service. One way to achieve this change is for health care teams to work and learn together more effectively. This research aimed to design and trial a facilitated learning programme with the aim of supporting general practice teams in fostering the characteristics of learning organizations. METHODS: This is an action research study. Qualitative data were captured during and after the trial from 40 participants in two multi-professional general practice teams within different Scottish health boards. Data were gathered using observations, semi-structured interviews and written learning notes. RESULTS: Taking part in the LPP was a positive experience of learning together as a practice and enhanced communication within the team was a particular outcome. External facilitation helped provide focus and reduce inter-professional barriers. Teams found working in small, mixed role discussion groups particularly valuable in understanding each others' perspectives. The active learning style of the LPP could be daunting at times but teams valued the chance to identify their own quality improvement goals. Teams introduced a number of changes to improve the quality of care within their practice as a result of their participation. CONCLUSION: This trial of the learning practice programme shows that, with facilitation and the appropriate input of resources, general practice teams can successfully apply learning organization principles to produce quality improvement outcomes. The study also demonstrates the value of action research in researching iterative change over time.

Cultivation of a learning culture in general practice: an educational intervention.

BACKGROUND: Increasing challenges are faced in primary care, including the increase in chronic disease and its management in the community. This paper describes an educational initiative developed to help local general practitioners (GPs) and primary care teams manage chronic conditions and address referral behaviour. The purpose of this study was to evaluate the initiative through the exploration of the experiences of the participants and providers. METHODS: A variety of methods was used to collect data for the evaluation. These included feedback from participants in the form of workshop evaluation questionnaires, semi-structured interviews of both participants and presenters and focus groups involving participants. RESULTS: Following analysis of the data, five themes were identified across all the methods used. This paper focuses on the key themes of 'practice change' and 'referral behaviour' as they relate to the specific aims and objectives of the initiative. The data indicated that participants had identified actual changes in practice following their participation in the educational initiative, including changes in individual practice and prescribing behaviour as well as in the provision of healthcare in the community. Changes identified in referral behaviour were evident in feedback from both participants and presenters in secondary care. Participants indicated proposed changes, including an anticipated reduction in referrals for dermatology with greater management of conditions in primary care, as well as a greater awareness of indications for early referral. The use of guidelines in aiding referral decisions was identified and the content and information required from referral letters was also a key area. CONCLUSION: The evaluation of this educational initiative has identified changes in practice in primary care and demonstrates how educational interventions can support and enhance future developments in primary care.