RESUMO
Preparative affinity chromatography of bovine serum amine oxidase (SAO) on aminohexyl (AH)-Sepharose was often associated with an unexpected irreversible SAO retention on the support. This particular enzyme immobilization, occurring without coupling reagents, was supposed to be due to a SAO ability to: (i) recognize alkylamine groups of the support as macro-molecularized substrate; (ii) catalyse their oxidation to the corresponding aldehydes, with release of NH3 and H2O2; and (iii) be immobilized on the activated support by a coupling between the nascent aldehyde groups and SAO free amine groups. This affinity immobilization procedure, with the self-activation of the support, being mild, allows by simple incubation for 24 h, the enzyme immobilization with the retention of 80% from original specific activity of free SAO. Immobilized SAO on AH-Sepharose microcolumns, viewed as a continuous flow-system reactor, was able to catalyse benzylamine oxidation for several weeks.
Assuntos
Amina Oxidase (contendo Cobre) , Cromatografia de Afinidade/métodos , Enzimas Imobilizadas/isolamento & purificação , Oxirredutases atuantes sobre Doadores de Grupo CH-NH/metabolismo , Sefarose/análogos & derivados , Animais , Benzilaminas/metabolismo , Bovinos , Fumaratos/metabolismo , Peróxido de Hidrogênio/metabolismo , Focalização Isoelétrica , Maleatos/metabolismo , Oxirredução , Peptídeos/metabolismo , Sefarose/metabolismo , Fatores de TempoRESUMO
Rhus vernicifera laccase (Japanese) was deglycosylated by treating it with exo- and endoglycosidases. In absence of unfolding agents deglycosylation does not exceed 32% while in denaturating conditions only 10% of saccharides are not digested. In the former case specific activity seems to be directly related to the amount of the residual carbohydrates. After deglycosylation in denaturing conditions the modified enzyme exhibits an electrophoretic component of about 60-65 Kd. A stabilizing effect of saccharide moiety on the catalytic site of native laccase was demonstrated. Antiserum raised against the enzyme recognized native Japanese, Vietnamese as well as deglycosylated laccase indicating that the two isoforms are immunologically similar.
Assuntos
Oxirredutases/química , Oxirredutases/imunologia , Plantas Tóxicas , Toxicodendron/enzimologia , Complexo Antígeno-Anticorpo/imunologia , Cobre/análise , Reações Cruzadas/imunologia , Eletroforese em Gel de Poliacrilamida , Glicosídeo Hidrolases , Glicosilação , Focalização Isoelétrica , Lacase , Oxirredutases/metabolismoRESUMO
1H-NMR relaxation measurements of Rhus laccase showed that a portion of the relaxivity was specifically abolished by less than stoichiometric EDTA. Another portion of relaxivity was removed by addition of N3(-) to the EDTA saturated enzyme. This treatment or selective removal of the Type 2 Cu left a large residual paramagnetic relaxivity (1700 M-1s-1) which was assigned to the Type 1 Cu. It is concluded that only a portion of the laccase relaxivity can be assigned to the Type 1 Cu and that this copper type does not behave homogeneously: the two fractions have different relaxivity, 5200 and less than or equal to 2400 M-1s-1 respectively.
Assuntos
Cobre/isolamento & purificação , Oxirredutases/isolamento & purificação , Plantas Tóxicas , Toxicodendron/enzimologia , Fenômenos Químicos , Química , Ácido Edético , Lacase , Espectroscopia de Ressonância Magnética , Proteínas de Plantas/isolamento & purificaçãoRESUMO
1. Spectroscopic and functional properties of Japanese-lacquer-tree (Rhus vernicifera) laccase were re-investigated, with special emphasis on the relationships between the different types of copper centres (Types 1, 2, and 3). 2. On removal of the Type 2 Cu(II), a decrease of absorbance occurred in the wavelength region above 650 nm (delta epsilon 750 = 300 M-1 . cm-1) and around 330 nm (delta episom 330 up to 2200 M-1 . cm-1). 3. Reductive titrations with ascorbic acid or ferrocyanide showed that the electron-accepting capacity of the partial apoprotein is one electron-equivalent lower than that of the native protein, i.e. the protein two-electron acceptor is present in the oxidized state in spite of absorbance loss at 330 nm. 4. The 330 nm chromophore apparently depends on the presence of both the Type 2 and the Type 3 copper in the oxidized state. 5. This finding may have implications in the relative location of Type 2 and 3 copper centres and on the redox behaviour of laccase.
Assuntos
Cobre , Oxirredutases , Plantas/enzimologia , Dicroísmo Circular , Cobre/análise , Lacase , Oxirredução , Plantas Tóxicas , Espectrometria de Fluorescência , Espectrofotometria , Toxicodendron/enzimologiaRESUMO
1. Redox titrations are reported of the metal centres in Japanese-lacquer-tree (Rhus vernicifera) laccase with ferrocyanide. 2. The redox potential of Type 1 Cu was found to increase with ferrocyanide concentration up to a limiting value similar to that for the Type 1 Cu in Type 2 Cu-depleted enzyme (which is independent of ferrocyanide concentration). 3. The redox potential of the two-electron acceptor (Type 3 Cu) is also independent of ferrocyanide concentration in Type 2 Cu-depleted enzyme and lower than values reported for the native enzyme. 4. The two-electron acceptor is present in the oxidized state in the Type 2 Cu-depleted enzyme, though the latter lacks the 330 nm absorption band. 5. The redox potential of Type 2 Cu also depends on ferrocyanide concentration, at least in the presence of azide. 6. The redox potentials are affected by freezing the solutions and/or addition of azide, the latter binding to Type 2 Cu with affinity dependent on the redox state of the two-electron acceptor.
Assuntos
Cobre , Ferrocianetos , Oxirredutases , Plantas/enzimologia , Sítios de Ligação , Oxirredução , Plantas Tóxicas , Nitrito de Sódio , Espectrofotometria , Toxicodendron/enzimologiaRESUMO
Electron spin-echo decay envelopes for types I and II copper of Rhus vernicifera laccase and for type II copper of procine ceruloplasmin have been studied. Nuclear modulation patterns show that imidazole is a ligand for all of them. The linear electric field effect (LEFE) in EPR was studied for type I copper in a laccase preparation from which type II had been removed. The symmetry of the site is near tetrahedral and the magnitude of the LEFE is correlated with the intensity of blue color.