Susceptibility to bacteriophages of Staphylococcus aureus strains received at the National Reference Centre for Staphylococci in Romania 1978-1992.

In order to determine the distribution among lytic groups of Staphylococcus aureus strains circulating in Romania 18,539 strains were phage typed in the National Reference Centre for Staphylococci between 1978 and 1992. Strains susceptible to group II phages prevailed, representing 28.28% of all phage typed strains. The distribution on lytic groups differed in terms of the type of the clinical isolate. The results are statistically analysed and compared with those obtained in Romania by Popovici et al. between 1966 and 1978 as well as with those reported for other geographical areas by other authors.

[Studies of oral microbiogenesis. II. Significance of staphylocci present in periodontal lesions, pulp gangrene and in the alveolus after tooth extraction]. / Studii de microbiogenoza orala. II. Semnificatia stafilococilor prezenti în leziunile parodontale, gangrena pulpara si în alveola dupa extractia dentara.

A study was carried out on the properties and significance of staphylococci isolated from the alveoli after dental extraction (12 strains), from parodontal pus (19 strains) and pulpar gangrene (15 strains). By their frequency, staphylococci of the Staphylococcus aureus have an etiologic significance in pulpar gangrene. Of particular importance for the diagnosis and epidemiology was the predominance of lysotype 80 among the staphylococci isolated from pulpar gangrene and lysotype 77 in healthy carriers. In 2 of 11 patients the coexistence of the same lysotype was found in the pulpar gangrene and nasal secretion.

[Value of different diagnostic tests for Micrococcaceae and their implication in pathology]. / Semnificatia a diferite teste de diagnostic al Micrococcaceae-lor si implicatia lor in patologie.

A number of 1431 products were examined and 200 coagulase-negative micrococcaceae strains were isolated and identified. On the basis of morphological aspects, enzymatic reactions and behavioural testing to antibiotics 18 strains (9%) were listed as Micrococcus and 182 (91%) as Staphylococcus strains. Efficient among the tests used for differentiating between the two genera were : microscopic aspects, pigmentogenesis, resistance to ClNa, anaerobic glucose fermentation, glycerol fermentation in the presence of erythromycin, reduction of nitrates to nitrites, and testing to novobiocin. Other tests, due to their variability, could only be used conjointly, and, finally, a further category had no diagnostic value, such as oxidase and catalase tests or the rhamnose fermentation reaction. Both the Micrococcus and the Staphylococcus strains were isolated from pathological cases and from healthy carriers, and can therefore be listed in the group of potential pathogens.

[Studies of oral microbiocenosis. I. Incidence and properties of staphylocci in young persons]. / Studii de microbiocenoza orala. I. Frecventa si proprietatile stafilococilor la tineri

The study of staphylococci in the buccodental washings of 117 second year students at the Faculty of Stomatology, all healthy youths aged 20-25 years, gave the following results: (I) stapphylococci were present in 90% of the students; (II) 50.66% were oral carriers of pathogenic staphylococcal strains; (III) in 23 cases two or more staphylococcal strains were found with different metabolic and biologic properties; (IV) part of the bucal lysozyme was of microbial origin since 95% of the coagulasopositive strains and 88% of the coagulasonegative strains produce lysozymes; (V) 35.23% of the strains yield staphylococcal active with regard to M. lysodeicticus; (VI) 56.19% of the staphylococcal strains were resistant to penicillin and/or tetracyclin, and 5 coagulopositive strains developed in media with a penicillin concentration of 800 u/ml; (VII) 85% of the coagulopositive staphylococci were sensitive to human anti-staphylococcal bacteriophages, the most frequent being phage type 77 of group III.