RESUMO
A simple prediction test for a combination of hyperthermia and chemotherapy checked on three experimental tumors and cells of the normal murine thymus is described. The testing procedure is based on checking the incorporation of labeled 125I 5-iodo-2-deoxyuridine into the DNA of tested cells affected solely by the enhanced temperature or by the cytostatic itself, or by a combination of both agents. The statistical analysis of repeated results demonstrated the reproducibility of the test. Testing of the potentiation effect of different non-cytostatic substances with enhanced temperatures also gave results corresponding to findings of other authors, performed in vivo or by the use of other techniques. Thus, this simple method is recommended for a screening, before considering the individual use of the combination of hyperthermia and chemotherapy in patients.
Assuntos
Antineoplásicos/uso terapêutico , Hipertermia Induzida , Neoplasias Experimentais/terapia , Animais , Linhagem Celular , Terapia Combinada , DNA de Neoplasias/biossíntese , Idoxuridina/metabolismo , Leucemia L1210/terapia , Camundongos , Camundongos Endogâmicos C57BL , Modelos BiológicosRESUMO
On the basis of an experimental model of the Lewis lung carcinoma a possibility was checked of obtaining basic kinetic data about the individual proliferation of tumour cells by a method of measuring the incorporation of labelles 125I 5-iodo-2-deoxyuridine intratumorously with a subsequent measurement of the retained activity in the tumour. These activity, which resides in the tumour for a time longer than 24 hours depends on the fraction of cells, that in the synthetic part of the cell cycle at the time of the injection.
Assuntos
Idoxuridina/metabolismo , Neoplasias Pulmonares/patologia , Animais , Divisão Celular , DNA de Neoplasias/metabolismo , Idoxuridina/administração & dosagem , Injeções , Cinética , Neoplasias Pulmonares/metabolismo , Camundongos , Camundongos Endogâmicos C57BLRESUMO
In a group of 14 individuals without tumor disease and 125 patients with verified malignant tumors we checked the applicability of determination of the pterins concentration in urine by the procedure described by RAO et al. [3]. In total of 242 examinations were performed. The results were compared with the thin-layer chromatography and on the basis of these comparisons the method was modified. Correlation of the clinical conclusion and the values of tests was obtained in 76% of cases. In patients with undoubtedly persisting tumors the correlation was found in 73%, in a group of patients with the leukemia and lymphomas in 97%, with mammary carcinoma in 81%. Evaluation made in these patients prior to the chemotherapy initiation correlated in 100%. This simple test offers a possibility of the use in differential diagnosis of malignant process or in the course of the screening.
