Translation and validation of a French version of the Xerostomia Inventory.

OBJECTIVE: This study aimed to assess the transcultural adaptation and psychometric properties of the French-language version of the Xerostomia Inventory (XI-Fr). METHODS: In total, 65 patients aged 65 years or older were recruited from three departments of a single French hospital. Patients had to have a Mini Mental State Examination score of 10 or more and be able to read and write French. The XI-Fr was administered to all patients after transcultural adaptation, at the start of the study, and again at 7 days after inclusion. We assessed reliability, acceptability, validity, internal consistency and reproducibility of the instrument in its French-language version. RESULTS: The XI-Fr showed good internal consistency (Cronbach's alpha 0.79) and good reliability (intra-class correlation 0.83) at 7 days. Convergent validity showed no relation between salivary flow and XI-Fr score, as with the original instrument. Discriminant validity showed a positive correlation between the XI-Fr and the GOHAI, but no difference for the MNA or miniGDS scores. CONCLUSION: The XI-Fr is a valid and reliable measure of xerostomia in French, with psychometric properties comparable to those of the original English-language version.

Orientation and Mobility During COVID-19 and the Effect on Psychosocial Functioning.

Introduction: This research explored the psychosocial effects observed during the COVID-19 pandemic as it relates to orientation and mobility (O&M) services for children with visual impairments. The survey asked professionals to identify what were their experiences in providing O&M instruction in-person and online and to describe their interactions with students and families during the fall of 2020. Methods: The online survey results included open-ended responses from 166 O&M specialists. The authors used thematic analysis to examine and code participants' qualitative responses. Results: Psychological and social effects were identified as a major theme across the participants for how they affected an individual's health, well-being, and ability to complete aspects of O&M lessons. Professionals were challenged with providing appropriate instruction given the risks associated with COVID. Additionally, children with visual impairments became more isolated and less physically active over time. Discussion: Professionals, students, and parents often placed their concerns aside with the idea that changes to O&M services were a temporary measure with the student's best interest in mind. As the pandemic became prolonged, the effects on individuals became more pronounced. Implications for Practitioners: These circumstances raise many questions about the effectiveness of virtual instruction in O&M and its effect on professionals, students, and families. The importance of O&M becomes much more evident when considering the isolation many students experienced as a result of quarantine events.

Use of novel chitosan hydrogels for chemical tissue bonding of autologous chondral transplants.

The objective of this study was to evaluate the effect of chemical tissue bonding (CTB) on adhesion strength, fluid permeability, and cell viability across a cartilaginous graft-host interface in an in vitro autologous chondral transplant (ACT) model. Chitosan-based cross-linkers; Chitosan-Rose Bengal [Chi-RB (Ch-ABC)], Chitosan-Genipin [Chi-GP (Ch-ABC)], and Chitosan-Rose Bengal-Genipin [Chi-RB-GP (Ch-ABC)] were applied to bovine immature cartilage explants after pre-treatment with surface degrading enzyme, Chondroitinase-ABC (Ch-ABC). Adhesion strength, fluid permeability and cell viability were assessed via mechanical push-out shear testing, fluid transport and live/dead cell staining, respectively. All three chitosan-based cross-linkers significantly increased the adhesion strength at the graft-host interface, however, only a statistically significant decrease in fluid permeability was noted in Chi-GP (Ch-ABC) specimen compared to untreated controls. Cell viability was maintained for 7 days of culture across all three treatment groups. These results show the potential clinical relevance of novel chitosan-based hydrogels in enhancing tissue integration and reducing synovial fluid penetration after ACT procedures in diarthoidal joints such as the knee and ankle. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1139-1146, 2016.

Resurfacing damaged articular cartilage to restore compressive properties.

Surface damage to articular cartilage is recognized as the initial underlying process causing the loss of mechanical function in early-stage osteoarthritis. In this study, we developed structure-modifying treatments to potentially prevent, stabilize or reverse the loss in mechanical function. Various polymers (chondroitin sulfate, carboxymethylcellulose, sodium hyaluronate) and photoinitiators (riboflavin, irgacure 2959) were applied to the surface of collagenase-degraded cartilage and crosslinked in situ using UV light irradiation. While matrix permeability and deformation significantly increased following collagenase-induced degradation of the superficial zone, resurfacing using tyramine-substituted sodium hyaluronate and riboflavin decreased both values to a level comparable to that of intact cartilage. Repetitive loading of resurfaced cartilage showed minimal variation in the mechanical response over a 7 day period. Cartilage resurfaced using a low concentration of riboflavin had viable cells in all zones while a higher concentration resulted in a thin layer of cell death in the uppermost superficial zone. Our approach to repair surface damage initiates a new therapeutic advance in the treatment of injured articular cartilage with potential benefits that include enhanced mechanical properties, reduced susceptibility to enzymatic degradation and reduced adhesion of macrophages.

An in vitro model for the pathological degradation of articular cartilage in osteoarthritis.

The objective of this study was to develop an in vitro cartilage degradation model that emulates the damage seen in early-stage osteoarthritis. To this end, cartilage explants were collagenase-treated to induce enzymatic degradation of collagen fibers and proteoglycans at the articular surface. To assess changes in mechanical properties, intact and degraded cartilage explants were subjected to a series of confined compression creep tests. Changes in extracellular matrix structure and composition were determined using biochemical and histological approaches. Our results show that collagenase-induced degradation increased the amount of deformation experienced by the cartilage explants under compression. An increase in apparent permeability as well as a decrease in instantaneous and aggregate moduli was measured following collagenase treatment. Histological analysis of degraded explants revealed the presence of surface fibrillation, proteoglycan depletion in the superficial and intermediate zones and loss of the lamina splendens. Collagen cleavage was confirmed by the Col II-3/4Cshort antibody. Degraded specimens experienced a significant decrease in proteoglycan content but maintained total collagen content. Repetitive testing of degraded samples resulted in the gradual collapse of the articular surface and the compaction of the superficial zone. Taken together, our data demonstrates that enzymatic degradation with collagenase can be used to emulate changes seen in early-stage osteoarthritis. Further, our in vitro model provides information on cartilage mechanics and insights on how matrix changes can affect cartilage's functional properties. More importantly, our model can be applied to develop and test treatment options for tissue repair.

Effect of sugar-free Red Bull energy drink on high-intensity run time-to-exhaustion in young adults.

Consuming sugar-free Red Bull energy drink before exercise has become increasingly popular among exercising individuals. The main purported active ingredient in sugar-free Red Bull is caffeine, which has been shown to increase aerobic exercise performance. The purpose of this study was to determine the effects of sugar-free Red Bull energy drink on high-intensity run time-to-exhaustion in young adults. Physically active university students (n = 17, 9 men, 8 woman; 21 +/- 4 years, 73.4 +/- 3.1 kg, 175.1 +/- 3.2 cm) participated in a double-blind, crossover, repeated-measures study where they were randomized to supplement with sugar-free Red Bull (2 mg x kg(-1) body mass caffeine or approximately 147 mg caffeine; 4 kcal/250 mL) and noncaffeinated, sugar-free placebo (lemon-lime flavored soft drink, tonic water, lime juice; 4 kcal/250 mL) separated by 7 days. Exercise capacity was assessed by a run time-to-exhaustion test at 80% Vo2max, perceived exertion was assessed immediately after exercise, and blood lactate was measured before and after exercise. There were no differences in run time-to-exhaustion (Red Bull: 12.6 +/- 3.8 minutes, placebo: 11.8 +/- 3.4 minutes), perceived exertion (Red Bull: 17.1 +/- 2.0, placebo: 16.6 +/- 1.8), or blood lactate between groups. In conclusion, sugar-free Red Bull energy drink did not influence high-intensity run time-to-exhaustion in young adults.

Smooth muscle alpha-actin and calponin expression and extracellular matrix production of human coronary artery smooth muscle cells in 3D scaffolds.

For a tissue-engineered coronary artery substitute to be a viable clinical option in the treatment of vascular diseases, it is necessary to use tissue-specific human cells. Coronary artery smooth muscle cells are the main resident cells in the tunica media of arteries. In this work, we examined the behavior and differentiation state of human coronary artery smooth muscle cells (HCASMCs) when cultured on 3D polyurethane scaffolds to fabricate hybrid vascular tissues. As the mechanical strength of the scaffold is an important element in engineered hybrid vascular substitutes, porous 3D polyurethane scaffolds fabricated using paraffin spheres and ammonium chloride particles were tested for their mechanical properties both in tension and in compression. The use of ammonium chloride particles as porogen generated scaffolds with superior mechanical properties, which are suitable for vascular tissue engineering. When seeded on uncoated, fibronectin-coated, and Matrigel-coated scaffolds, HCASMCs were well spread and started producing collagen as judged by histochemical analysis but appeared to lack elastin production. Fibronectin coating appeared to promote the infiltration of HCASMCs into the scaffold better than Matrigel coating but did not appear to affect the expression of collagen and elastin. Western blot analyses after successful cell recovery from the scaffolds indicated that HCASMCs, after culturing for 4 and 7 days, expressed similar amounts of smooth muscle alpha-actin and calponin regardless of extracellular matrix coating. Taken together, our data showed that the behavior and differentiation phenotype of HCASMCs can be analyzed after culture in 3D polyurethane scaffolds to establish appropriate conditions that will favor the fabrication of hybrid-engineered vascular substitutes.

Interactions of coronary artery smooth muscle cells with 3D porous polyurethane scaffolds.

One strategy in vascular tissue engineering is the design of hybrid vascular substitutes where vascular cells infiltrate biostable porous scaffolds that provides favorable environment for guided cell repopulation and acts as a mechanically supporting layer after the tissue regeneration process. The aim of the present work was to study the interaction of human coronary artery smooth muscle cells (HCASMC) with 3D porous polyurethane scaffolds. We therefore fabricated porous and highly interconnected 3D polyurethane scaffolds that can promote HCASMC attachment, proliferation, and migration. SEM and microCT studies of the fabricated scaffolds showed that the current scaffolds had highly open and interconnected pore structures, with an average porosity of 84%. HCASMC interaction on polyurethane films revealed that cells adhere and express specific marker proteins (vinculin and h-caldesmon). This expression was further enhanced by coating the polyurethane with Matrigel. On uncoated 3D scaffolds, dense spherical aggregates of cells were often encountered with little adhesion of individual cells alongside the struts of the scaffold, independent of the porogens used. In contrast, when cultured on Matrigel-coated scaffolds, cell numbers quickly increased after 14 days and spread along the entire scaffold. At the upper scaffold surface, elongated cells were seen adhering to one another and also to the scaffold surface. These cells were elongated, aligned in parallel and contained abundant F-actin bundles suggesting a differentiated contractile phenotype. Deep into the scaffold, cells were encountered that formed actin-rich lamellipodial extensions spreading along the strut and lacked stress fibers, suggesting active cell migration along the substrate.

Polyurethane biomaterials for fabricating 3D porous scaffolds and supporting vascular cells.

Successful tissue engineering of vascular grafts largely depends on synthetic scaffolds that support the survival, proliferation, and differentiation of seeded cells. To investigate the utility of polyurethanes for vascular tissue engineering, three-dimensional porous polyurethane scaffolds with highly interconnected pore structures were fabricated by a pressure differential/particulate leaching technique. Ammonium chloride and paraffin porogens were prepared to fabricate the scaffolds. Grinding of ammonium chloride resulted in particulates with uniform particle sizes but irregular shapes. Paraffin particulates made by a dispersion method, on the other hand, had spherical shapes and uniform particle sizes. Polyurethane scaffolds fabricated from these particulates had open faced, highly interconnected channels that could allow cellular infiltration and nutrient delivery. Human coronary artery smooth muscle and endothelial cell interactions with polyurethane surfaces revealed these biomaterials to maintain the contractile phenotype of human coronary artery smooth muscle cells and the formation of endothelial monolayers. During longer culture times, surface modification with cell adhesive extracellular matrix (ECM) protein promoted vascular cell proliferation, maintenance of the differentiated phenotype and endothelial monolayer integrity. Our results suggest that these polyurethanes, in conjunction with cell adhesive ECM proteins, could also support vascular cells in three-dimensional bioreactor-based culture conditions.