Polarized light sensitivity in Pieris rapae is dependent on both color and intensity.

There is an ever increasing number of arthropod taxa shown to have polarization sensitivity throughout their compound eyes. However, the downstream processing of polarized reflections from objects is not well understood. The small white butterfly, Pieris rapae, has been demonstrated to exploit foliar polarized reflections, specifically the degree of linear polarization (DoLP), to recognize host plants. The well-described visual system of P. rapae includes several photoreceptor types (red, green, blue) that are sensitive to polarized light. Yet, the roles and interaction among photoreceptors underlying the behavioral responses of P. rapae to stimuli with different DoLP remain unknown. To investigate potential neurological mechanisms, we designed several two-choice behavioral bioassays, displaying plant images on paired LCD monitors, which allowed for independent control of polarization, color and intensity. When we presented choices between stimuli that differed in either color or DoLP, both decreasing and increasing the intensity of the more attractive stimulus reduced the strength of preference. This result suggests that differences in color and DoLP are perceived in a similar manner. When we offered a DoLP choice between plant images manipulated to minimize the response of blue, red, or blue and red photoreceptors, P. rapae shifted its preference for DoLP, suggesting a role for all of these photoreceptors. Modeling of P. rapae photoreceptor responses to test stimuli suggests that differential DoLP is not perceived solely as a color difference. Our combined results suggest that Prapae females process and interpret polarization reflections in a way different from that described for other polarization-sensitive taxa.

Polarization of foliar reflectance: novel host plant cue for insect herbivores.

Insect herbivores exploit plant cues to discern host and non-host plants. Studies of visual plant cues have focused on colour despite the inherent polarization sensitivity of insect photoreceptors and the information carried by polarization of foliar reflectance, most notably the degree of linear polarization (DoLP; 0-100%). The DoLP of foliar reflection was hypothesized to be a host plant cue for insects but was never experimentally tested. Here, we show that cabbage white butterflies, Pieris rapae (Pieridae), exploit the DoLP of foliar reflections to discriminate among plants. In experiments with paired digital plant images, P. rapae females preferred images of the host plant cabbage with a low DoLP (31%) characteristic of cabbage foliage over images of a non-host potato plant with a higher DoLP (50%). By reversing the DoLP of these images, we were able to shift the butterflies' preference for the cabbage host plant image to the potato non-host plant image, indicating that the DoLP had a greater effect on foraging decisions than the differential colour, intensity, or shape of the two plant images. Although previously not recognized, the DoLP of foliar reflection is an essential plant cue that may commonly be exploited by foraging insect herbivores.