[Hyperbaric oxygenation in the treatment of facial neuritis]. / Giperbaricheskaia oksigenatsiia pri lechenii nevrita litsevogo nerva.

The effectiveness of hyperbaric oxygenation (HBO) in a multiple modality therapy versus the conventional treatment of patients with facial neuritis was investigated. HBO was used in the treatment of 42 patients. Twenty-nine patients with a similar picture of the diseases treated by conventional methods without the use of HBO constituted the control group. The inclusion of hyperbaric oxygenation in to the multiple modality treatment of patients with facial neuritis increased the efficacy of therapy and reduced the period needed for the restoration of the function of the damaged nerve.

[Regulatory region of fr phage replicase cistron. II. Isolation and structure of specific fr RNA fragments]. / Reguliatornyi uchastok tsistrona replikazy faga fr. II. Vydelenie i struktura spetsificheskikh fragmentov RNK fr.

A new set of short RNA templates has been prepared for functional studies in initiation of translation in vitro. Number of individual RNA fragments which contain complete or part of the initiatory region of phage fr replicase cistron were isolated from complex fr RNA--fr coat protein. Their primary structure were determined by using standard fingerprint technique and rapid gel sequencing. Secondary structure of several RNA fragments and their binding activity with phage fr and MS2 coat proteins has been also studied.

[Regulatory region of the MS2 phage RNA replicase cistron. Accesibility of MS2 RNA specific fragment to T1 RNAse digestion and chemical modification with kethoxal]. / Reguliatornyi uchastok tsistrona replikazy RNK faga MS2. Dostupnost' Spetsificheskogo fragmenta RNK faga MS2 deistviiu RNKazy T1 i khimicheskoi.

Partial digestion with T1 RNAase and chemical modification with kethoxal were used to study stability of two hairpin in the proposed secondary structure of the functionally active MS2 RNA fragment MS2 R(--53 leads to 6), containing the regulatory region of the phage replicase cistron. Analysis of the products obtained after the above treatments showed that T1 RNAase and kethoxal attacked predominantly the guanosine residues in the hairpin b of the MS2 R(--53 leads to 6). This implies that in contrast to the structurally stable hairpin a of the polynucleotide, hairpin b appears to be more labile and may exist under the present experimental conditions in equilibrium with its open form. The data of the competition experiments demonstrated that the kethoxal modified MS2 R(-53 leads to 6) and shorter polynucleotide MS2 R(-53 leads to-11) obtained from MS2 R(-53 leads to 6) after T1 RNAase digestion failed to bind with MS2 coat protein. The relatively unstable hairpin b region in the polynucleotide MS2 R(-53 leads to 6) is suggested to play essential role in the complex formation.