RESUMO
AIM: Study infection level by West Nile virus (WNV) of donors living on the territory of West Nile fever (WNF) foci with long-term activity, and evaluate the possibility of non-transmissible transfer of the causative agent during use of donor blood. MATERIALS AND METHODS: Blood sera (432) collected in August and September 2012 in Volgograd and Volzhsky cities were studied by PCR and EIA. RESULTS: Antibodies to WNV were detected in 16.2% (70/432); 8.8% (38/432) donors had IgG; 0.93% (4/432)--IgM and 6.5% (28/432)--IgG and IgM antibodies simultaneously. WNV RNA was detected in one of the samples in the last group. Thus, 5 donors (1.1%, 5/432) were at the early stages after infection. CONCLUSION: The data obtained give evidence on the necessity to execute control of donor blood by PCR and EIA in WNF foci with long-term activity.
Assuntos
Anticorpos Antivirais/sangue , Doadores de Sangue , Infecção Focal , RNA Viral/sangue , Febre do Nilo Ocidental/epidemiologia , Monitoramento Epidemiológico , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Federação Russa/epidemiologia , Febre do Nilo Ocidental/imunologia , Febre do Nilo Ocidental/transmissão , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/genética , Vírus do Nilo Ocidental/imunologia , Vírus do Nilo Ocidental/isolamento & purificaçãoRESUMO
The prevalence of Kemerovo virus in ixodid ticks collected in 2008-2012 from 11 regions of the Russian Federation was investigated by real-time reverse-transcription PCR (RT-PCR). The presence of Kemerovo virus in Ixodes persulcatus, Ixodes ricinus, and Dermacentor reticulatus was confirmed. Virus prevalence depended on the region and varied from zero to 10.1%.
Assuntos
Vetores Aracnídeos/virologia , Ixodidae/virologia , Orbivirus/isolamento & purificação , Animais , Sequência de Bases , Dados de Sequência Molecular , Orbivirus/classificação , Orbivirus/genética , Filogenia , Federação RussaRESUMO
AIM: Determine the duration of persistence of IgM and IgG in reconvalescents of West Nile fever (WNF) 1 year after the disease in southern regions of Russia and evaluate effectiveness of PCR method for acute infection diagnostics. MATERIALS AND METHODS: Blood sera of 87 patients with WNF diagnosis was studied for the presence of West Nile virus (WNV) RNA and IgM and IgG by PCR and EIA. Samples of the first sera were collected in 2010 at days 2 - 30 after the onset of the disease, samples of the second sera--at days 5 - 23 and third--264 - 385 days later. RESULTS: During the first 2 weeks of the disease WNV RNA was detected in more than 50% of patients. In all the first sera IgM at titers of ≥ 1:800 were detected. Seroconversion of IgG titers of 4 and more times was observedin 83% (30/36) of patients. In 2011 IgG were detected in 91% of reconvalescents (79/87), IgM--in 57% (50/87), and in 25% (22/87) IgM titers were ≥ 1:800. CONCLUSION: The results obtained give evidence on the necessity of using several diagnostic criteria simultaneously for the confirmation of WNF clinical diagnosis.
