RESUMO
It was investigate the content of zinc, magnesium and copper in granulocytes and blood serum of the rabbits, that were injected with substances, that change the functional state of adrenal cortex, sympathetic-adrenal and parasympathetic nervous systems. It has been found that adrenaline, prednisolone and pilocarpine caused the multidirectional changes of these metals content in cells and in extracellular space. In this significant increase of zinc concentration by 33 - 42%, magnesium--by 33 -50%, and also decrease of copper content by 25-50% was observed in granulocytes of animals after adrenal hormones injections. Under the influence of cholinomimetics content of zinc and magnesium were essential decreased in granulocytes of the rabbits, by 58% and by 33% respectively, and content of copper was risen by 43% (P < 0.001). The opposite pattern was observed in serum. Adrenaline and prednisolone prescription caused a significant decrease of zinc concentration by 20-24%, magnesium--by 22-33%, and increase of copper content by 36-43%. Pilocarpine injection caused a decrease of zinc and magnesium content by 28 and 33% (P < 0.01) respectively, and an increase of copper concentration by 43% (P < 0.001). The obtained results also indicate a synergistic relationship between zinc and magnesium in cells, but antagonistic--these metals with copper.
Assuntos
Cobre/sangue , Granulócitos/metabolismo , Magnésio/sangue , Zinco/sangue , Córtex Suprarrenal/efeitos dos fármacos , Córtex Suprarrenal/fisiologia , Animais , Sistema Nervoso Autônomo/efeitos dos fármacos , Sistema Nervoso Autônomo/fisiologia , Cátions Bivalentes , Colinérgicos/farmacologia , Epinefrina/farmacologia , Espaço Extracelular/química , Espaço Extracelular/efeitos dos fármacos , Espaço Extracelular/metabolismo , Glucocorticoides/farmacologia , Granulócitos/química , Granulócitos/efeitos dos fármacos , Sistema Nervoso Parassimpático/efeitos dos fármacos , Sistema Nervoso Parassimpático/fisiologia , Pilocarpina/farmacologia , Prednisolona/farmacologia , Coelhos , Sistema Nervoso Simpático/efeitos dos fármacos , Sistema Nervoso Simpático/fisiologia , Simpatomiméticos/farmacologiaRESUMO
Dry seeds of Crepis capillaris L. were treated with N-nitroso-N-methyl urea (NMU) and with dioxane (DO) an organic solvent, at pH 7.0 and pH 5.7. The treatment with NMU only was used as a positive control. Three concentrations of NMU were applied. The cytogenetic activity of NMU was found to considerably decrease at two values of pH, while NMU was solved in DO. The relationship between different types of chromosome aberrations remained unchanged in this case.
