[Determination of nystatin component composition using HPLC and TLC with densitometry]. / Opredelenie komponentnogo sostava nistatina metodami VEZhKh i TSKh s ispol'zovaniem densitometrii.

The component composition of nystatin produced by an improved strain of Streptomyces noursei was determined by HPLC on Milichrom chromatograph (USSR). It was shown that the antibiotic consisted of nystatins A1, A2, A3 and B and admixture substances. The data appeared to be in good agreement with the results of the complex TLC investigation, by using densitometry. The component composition of the samples was evidenced by SIEAP mass spectrometry. Physiochemical and biological characteristics of separate components are presented.

[Synthesis and biological activity of aminophenylphosphonic derivatives of levorin]. / Poluchenie i biologicheskaia aktivnost' aminofenilfosfonistykh proizvodnykh levorina.

Reactions of levorin, a polyenic macrolide antibiotic, with aromatic aldehydes and hypophosphorous acid resulted in formation of its amino phenylphosphonium derivatives. Physicochemical and biological properties of the derivatives were studied. The levorin amino phenylphosphonium derivatives were shown to be low toxic and have antifungal and antiviral activities.

[Effect of adenosine on hemodynamics in cats]. / Vliianie adenozina na gemodinamiku u koshek.

In anesthetized cats, i.v. administration of adenosine reduced the mean systemic blood pressure and systemic vascular resistance while increasing the cardiac output and coronary blood flow.

[Isolation and study of the properties of the hemagglutinins produced by Streptomyces badius actinomycetes]. / Vydelenie i izuchenie nekotorykh svoistv gemaggliutininov, produtsirumykh aktinomitsetami Streptomyces badius.

Hemagglutinin inducing polyagglutination of erythrocytes in the presence of the group blood sera was isolated from the supernatant of the culture fluid of Streptomyces badius 0626 by precipitation with ammonium sulfate and subsequent chromatography on Sephadex. Hemagglutinin proved to be a protein containing no lipid or carbohydrate components and possessing no bacterioagglutinating ability. Examination of the culture fluid supernatants of 150 cultures did not reveal any strains definitely exhibiting the transformation capability for human blood groups.

[Effect of chemical modification on proteinase interaction with inhibitors, on their coagulability and acute toxicity]. / Vliianie khimicheskoi modifikatsii na vzaimodeistvie proteinaz s ingibitorami, na ikh koaguliruiushchuiu aktivnost' i ostruiu toksichnost'.

A comparative study of terrylytin, trypsin and products of their covalent binding to human serum albumin was carried out. Modification caused a decrease of the affinity of both enzymes for proteinase inhibitors from the blood. The inhibition constant for terrylytin was increased 3-4-fold, that for trypsin - by 2 or 3 orders. The recalcification time of human blood plasma in the presence of terrylytin remained practically unchanged after enzyme chemical modification. Trypsin binding to albumin decreased its coagulability. Preparations of modified enzymes were characterized by a decreased acute toxicity, this effect being especially well-pronounced in the case of trypsin. The decrease of terrylytin toxicity due to modification can be accounted for by a decrease in its affinity for alpha2-macroglobulin. The decrease of trypsin toxicity is apparently due to a decrease of its coagulability due to binding to albumin.