RESUMO
We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.
Assuntos
Neoplasias da Mama/metabolismo , Carcinoma Ductal de Mama/metabolismo , Perfilação da Expressão Gênica/métodos , Fixação de Tecidos/métodos , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Carcinoma Ductal de Mama/genética , Carcinoma Ductal de Mama/patologia , Feminino , Fixadores/química , Formaldeído/química , Expressão Gênica , Humanos , Pessoa de Meia-Idade , Estabilidade de RNA , Kit de Reagentes para DiagnósticoRESUMO
Inflammatory breast cancer is characterized by high malignancy, early and rapid lymphogenic and hematogenic metastasizing, and high mortality. The diagnosis of this form of cancer is based fully on the clinical criteria. Whole transcriptome analysis of tumor tissue specimens from patients with inflammatory breast cancer detected 137 differentially expressed mRNA (17 genes with low expression and 120 with high expression). Genes involved in the organization of inflammatory process, chemotaxis, and transcription regulation were active in the process of pathogenesis of inflammatory breast cancer.
Assuntos
Regulação Neoplásica da Expressão Gênica , Neoplasias Inflamatórias Mamárias/genética , Proteínas de Neoplasias/genética , RNA Mensageiro/genética , Transcriptoma , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Quimiotaxia/genética , Feminino , Perfilação da Expressão Gênica , Humanos , Neoplasias Inflamatórias Mamárias/patologia , Metástase Linfática , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Análise de Sequência com Séries de OligonucleotídeosRESUMO
The paper considers the morphological features of a rare hyalinizing trabecular tumor of the thyroid, which is of follicular cell origin. The signs of invasive tumor growth into to the intrinsic capsule and its blood vessels were observed in 2 of 7 cases. There was a positive immunohistochemical reaction of tumor cells with antibodies to thyroglobulin and neuron-specific enolase and a negative one to calcitonin, galectin 3, cytokeratin 19, and HBME-1. The differential diagnosis of a tumor and papillary and medullary carcinomas of the thyroid, as well as its malignancy potential are discussed in the paper.
Assuntos
Carcinoma Medular/metabolismo , Carcinoma Medular/patologia , Carcinoma Papilar/metabolismo , Carcinoma Papilar/patologia , Proteínas de Neoplasias/metabolismo , Neoplasias da Glândula Tireoide/metabolismo , Neoplasias da Glândula Tireoide/patologia , Adulto , Carcinoma Medular/irrigação sanguínea , Carcinoma Papilar/irrigação sanguínea , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Estudos Retrospectivos , Neoplasias da Glândula Tireoide/irrigação sanguíneaRESUMO
Efficacy of application of intraarterial erythrocytes infusion, photomodified by using low-energy laser irradiation, was proved, basing on comparative analysis of treatment results of the different groups of patients with obliterating disease of the lower extremities vessels.
