[Method of developing alkaloids of different structural types on a thin layer of sorbent]. / Metod proiavleniia alkaloidov razlichnykh strukturnykh tipov v tonkom sloe sorbenta.

A new method for developing alkaloids of the piperidine, pyridine, isochinoline, indole, purine and chinoline groups in a thin sorbent layer has been developed. This method is based on the use of the Dragendorf reagent, subsequent disintegration of the stained complex, and chromatogram treatment with a sodium thiosulfate ammonia solution. The method makes it possible to measure quantitatively alkaloids of various structures, using UV-spectrometry after their elution. The relative error is 1.9-5.5% in three parallel measurements.

Variability of alkaloid spectrum in early stages of development of Glaucium flavum Crantz.

The alkaloid spectrum of the yellow horned poppy G. flavum was studied in early stages of development of this plant by methods of thin-layer chromatography and spectrophotometry. It was established that gradual formation of the alkaloid complex takes place in parallel with growth differentiation of the organs during seed germination and the formation of seedlings, which is due to the de novo biosynthesis (in a specific sequence) of alkaloids of various structural types: first 1,2,9,10-substituted aporphines, then protopines, and, finally 1,2,10,11-substituted aporphines, benzophenanthridine, as well as morphinans. It was found that glaucine predominates among the alkaloids of the seeds and in the above-ground organs of seedlings of different ages, while protopine predominates in the subsoil organs. The data obtained on the nature of the formation of the alkaloid complex suggest that the aporphines are the phylogenetically oldest compounds among alkaloids of other groups of this plant.