RESUMO
Conservation of drug-producing plant genetic resources in an active germplasm bank. In August 1983 a project began at the National Genetic Resources Center (CENARGEN) in Brasília, Brazil , to establish an Active Germplasm Banl of drug-producing plants. This Bank seeks the permanent conservation of important species as well as basic research on biological aspects related to plant improvement. A living plant collection at presen occupies an area of 6,000m2 , which will be expanded as needed, and now contains over 200 accessions. The species being conserved are those used as home remedies by the population of the Distrito Federal, and it will also conserve 10-20 of th most important species of each of the five geographical regions of Brazil, as well as ca. 20 species introduced from other countries. The field collection is being studied to provide basic information and material for distribution to research centers specializing in pharmacological, agronomic and other related research. Information being collected includes reproductive biology an seed technology. Standard germplas, routines for documentation, registration, cleaning, quarantining an packaging of seeds are being used. It is expected that this Active Germplasm Banl will becomen a model for others that must be created elsewhere in Brazil so that the disappearance of important drug-producing plant species can be avoided.
Em agosto de 1983, o Centro Nacional de Recursos Genéticos (GENARGEN) iniciou um projeto de estabelecimento de um Banco Ativo de Germoplasma de Plantas Medicinais. Sua finalidade é a conservação permanente de importantes espécies de plantas bem como o estudo de aspectos biológicos relacionados ao melhoramento genético. Uma coleção ativa de plantas no campo, já ocupa uma área de 6.000m2, contendo mais de duzentos acessos. As espécies sendo conservadas são aquelas usadas pela população do Distrito Federal, mas serão também conservadas as 10-20 espécies mato importantes de cada região do país, além de 20 espécies introduzidas. A coleção ativa está sendo estudada para se obter suficientes informações e material a ser distribuído aos centros de pesquisas farmacológicos, agronômicos e outros relacionados. As informações sendo obtidas incluem biologia reprodutiva e tecnologia de sementes. Rotinas de manipulação de germoplasma tal como documentação, registro, fitossanidade, quarentena e empacotamento de sementes estão sendo empregadas. Espera-se que esse banco ativo de germoplasma seja um protótipo para outros que deverão ser criados r todo o país, para evitar o desaparecimento de espécies importantes de plantas produtoras de drogas.
Assuntos
Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Diabetes Mellitus , Escleredema do AdultoRESUMO
Mast cells contain heparin, histamine, hydrolytic enzymes, and possibly serotonin in metachromatic cytoplasmic granules, and are not visualized in routine histological preparations. Special fixation, frozen sections, and toluidine blue staining are essential for counting the number of mast cells in tissue sections. Histological preparations for counting mast cells were made from arteries of the circle of Willis in persons who died after chest or abdominal trauma (control group) and in patients who had subarachnoid hemorrhage (SAH) after aneurysm rupture. The arteries were removed within 6 hours of death, taking care to avoid damage to their structure, and were immersed in the fixative solution. This preliminary note, reporting findings in only a few cases, is justified by the interesting discovery of a marked increase in mast cell population in the muscular layer of arteries after SAH. The series is small because of the difficulty in obtaining suitable material, since mast cells virtually disappear when autopsy is performed later than 6 hours after death. It is concluded from this study that there is an increase of mast cell population in cerebral arterial walls after SAH, mainly in the muscular layer, and that the number of mast cells is higher in arteries closer to the aneurysm.
