Determinants of farms' antibiotic consumption - A longitudinal study of pig fattening farms in Germany.

As high consumption of antibiotics in livestock production poses risks to public health, Germany has implemented a monitoring system to decrease their administration to farm animals. Data from 1,984 German pig farms are used to describe prescription trends for different antibiotic subclasses between Autumn 2017 and Autumn 2019. A panel Tobit model with control function approach is implemented to identify determinants of antibiotic consumption, where variables studied include farm, farmer, and county characteristics as well as weather variables. The overall quantity of prescribed antibiotics has been stable but with seasonal fluctuations and a shift away from critically important antibiotics used. Biosecurity factors such as livestock farm density in a county and pigs per farm are shown to be important drivers of antibiotic consumption. In addition, the number of cold days within a season increases antibiotic consumption but precipitation and the number of hot days have no significant effect.

Immediate effects of whole blood donation on the endothelial surface layer and glycocalyx shedding.

BACKGROUND: The endothelium plays a pivotal role in regulating microvascular function, especially in situations associated with acute blood loss. Whether blood donation and the associated volume loss affects the dimensions of the endothelial surface layer (ESL) and glycocalyx integrity remains unknown. MATERIALS AND METHODS: This study was designed to determine real-time ESL and perfusion measurements of the sublingual microcirculation using sidestream dark field imaging performed in healthy subjects shortly before and after a donation of 500 mL whole blood. A novel image acquisition and analysis software (GlycoCheck™) automatically calculated the perfused boundary region (PBR), an inverse parameter for red blood cell (RBC) penetration into the ESL, in vessels between 5 and 25 µm in diameter. Microvascular perfusion was measured by RBC filling percentage. Soluble glycocalyx components were determined in the peripheral circulation. RESULTS: There was no significant immediate effect of whole blood donation on PBR and RBC filling percentage. Linear regression analysis revealed a distinct association between change in PBR and change in RBC filling percentage (regression coefficient ß: -0.040; 95% confidence interval: -0.049 to -0.030; p<0.001). A significant reduction in plasma heparan sulphate (1,329±316 vs 1,237±275 ng/mL, p=0.005) and hyaluronan (94±18 vs 90±16 ng/mL, p=0.002) was noted, while syndecan-1 levels (30 [23-50] vs 29 [24-46] ng/mL, p=0.282) remained unchanged. DISCUSSION: Dimensions and integrity of the ESL appear to remain stable following a 500 mL whole blood donation and reflect its ability to ensure microvascular function and perfusion.