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1.
In Vivo ; 20(1): 125-7, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16433040

RESUMO

HIV protease inhibitors are antiretroviral drugs able to prevent production of infectious particles. It has been shown that these protease inhibitors are able to inhibit cancer-promoted angiogenesis in patients affected by Kaposi's sarcoma. A preliminary phase I study on dogs with stage III splenic hemangiosarcoma was designed in order to evaluate the efficacy and toxicity of the protease inhibitor Indinavir to delay the progression of this advanced neoplasm. The results suggest that Indinavir is potentially beneficial in dogs affected by microscopic residual disease.


Assuntos
Doenças do Cão/tratamento farmacológico , Inibidores da Protease de HIV/uso terapêutico , Hemangiossarcoma/tratamento farmacológico , Indinavir/uso terapêutico , Neoplasias Esplênicas/tratamento farmacológico , Animais , Doenças do Cão/metabolismo , Cães , Fator 2 de Crescimento de Fibroblastos/biossíntese , Hemangiossarcoma/metabolismo , Hemangiossarcoma/veterinária , Neoplasias Esplênicas/metabolismo , Neoplasias Esplênicas/veterinária , Fator A de Crescimento do Endotélio Vascular/biossíntese
2.
In Vivo ; 19(6): 1051-4, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16277021

RESUMO

Primary melanoma of the gastrointestinal tract is a rare, highly malignant neoplasm that is associated with an unfavorable long-term prognosis. Animal studies are needed to further characterize this tumor and to develop new and more effective protocols. A spontaneous canine intestinal melanoma is described which, because of its local aggressive behavior and advanced stage, was not treatable with conventional strategies, thus being a potential candidate for investigational trials.


Assuntos
Abdome/diagnóstico por imagem , Doenças do Cão/diagnóstico , Neoplasias Intestinais/veterinária , Melanoma/veterinária , Animais , Biópsia , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/patologia , Cães , Endossonografia/veterinária , Neoplasias Intestinais/diagnóstico , Neoplasias Intestinais/diagnóstico por imagem , Neoplasias Intestinais/patologia , Masculino , Melanoma/diagnóstico , Melanoma/patologia , Estadiamento de Neoplasias/veterinária , Prognóstico
3.
Anticancer Res ; 25(1B): 669-74, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15816644

RESUMO

BACKGROUND: Chronic anemia is a well-recognized complication of both cancer and cytotoxic treatments and is associated with symptoms (e.g., fatigue, dyspnea) that may induce or exacerbate functional deterioration. The use of recombinant human erythropoetin (rHuEPO epoetin alfa) clearly increased haemoglobin (Hb) levels, decreased transfusion needs and allowed recovery of quality of life in anemic cancer patients (pts) undergoing chemotherapy (CT). The purpose of this open-label, non randomized, pilot study was to assess the safety and efficacy of an intensive 19-day epoetin alfa treatment in anemic patients with solid tumors receiving chemotherapy. TREATMENT: patients received a single induction s.c. dose of epoetin alfa 40,000 IU day 1 and twice a dose of 10,000 IU s.c. (8.00 a.m.- 8:00 p.m.) on days 3, 5, 8, 10, 12, 15, 17 and 19. The total dose of epoetin alfa per patient was 200, 000 IU. Iron supplementation: 125 mg i.v. days and 8. Soluble transferrin receptor (sTfR) levels were performed on days 1,8 and 15. This epoetin induction regimen was not followed by an epoetin maintenance therapy. PATIENTS: Twenty-nine anemic (Hb< or =11.5 g/dL) pts with non myeloid malignancies undergoing CT were included in the study. RESULTS: At baseline the mean Hb level was 9.41 g/dl. On day 8, the mean Hb level increased to 10.07 g/dl (p<0.0001), reaching 10.68 g/dl on day 15 (p<0.0001). On days 22 and 29, the mean Hb levels increased to 10.93 and 11.05 g/dl, (p=0.002 and 0.033, respectively). No patient received blood transfusions. The global mean increase of Hb level was 1.64 g/dl (basal to d 29). It was defined as a major response: an increase of Hb levels > 1.5 g/dl. A rate of 62% (18/29 patients) of major responses was observed on day 21. Moreover, 25/29 patients (86.2%) presented an increase of Hb levels > 1 g/dl after 21 days. On days 8 and 15, the mean sTfR levels had increased significantly ( p=0.021 and 0.001, respectively). The increase of mean sTfR level after 15 days correlated significantly with the increase of mean Hb level in the first two weeks of epoetin therapy (p=0.05). Epoetin alfa has been well tolerated so far in the study. CONCLUSION: The results of the present study suggest that an induction dose of 40,000 IU of epoetin alfa, followed by 8 maintenance doses of 20,000 IU each, may improve the standard response in terms of both time to response and Hb increase. Moreover, the Hb levels seemed to increase after epoetin therapy discontinuation (d22-29).


Assuntos
Anemia/induzido quimicamente , Antineoplásicos/uso terapêutico , Eritropoetina/uso terapêutico , Hematínicos/uso terapêutico , Neoplasias/tratamento farmacológico , Adulto , Idoso , Epoetina alfa , Feminino , Hemoglobinas/metabolismo , Humanos , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Receptores da Transferrina/sangue , Proteínas Recombinantes , Fatores de Tempo , Resultado do Tratamento
4.
In Vivo ; 18(5): 553-4, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15523892

RESUMO

Scrotal leiomyosarcoma is a rare tumour arising from the dartos layer: We describe a case of scrotal leiomyosarcoma in a 40-year-old man. The patient was treated by a wide surgical excision and no recurrence has been recognized 36 months later. A review of the literature is presented, summarizing the principal clinical and morphological characteristics of this rare tumor.


Assuntos
Neoplasias dos Genitais Masculinos/patologia , Leiomiossarcoma/patologia , Escroto/patologia , Adulto , Biomarcadores Tumorais/metabolismo , Neoplasias dos Genitais Masculinos/metabolismo , Neoplasias dos Genitais Masculinos/cirurgia , Humanos , Técnicas Imunoenzimáticas , Leiomiossarcoma/metabolismo , Leiomiossarcoma/cirurgia , Masculino , Estadiamento de Neoplasias , Escroto/cirurgia , Resultado do Tratamento
5.
Anticancer Res ; 24(5B): 3157-9, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15510605

RESUMO

Pleomorphic lipomas are rare benign tumours that can resemble a variety of malignant tissue tumour on histological examination. We describe a case of pleomorphic lipoma arising on the posterior aspect of the neck of a 70-year-old man, successfully treated by surgical excision. A review of the literature is presented, summarizing the principal clinical and morphological characteristics of this rare tumor.


Assuntos
Neoplasias de Cabeça e Pescoço/patologia , Lipoma/patologia , Idoso , Humanos , Imuno-Histoquímica , Masculino
6.
Anticancer Res ; 23(6C): 4901-5, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14981943

RESUMO

Defects in the apoptotic pathway represent a critical element in the progression of neoplastic disease and may also concur to determine treatment efficacy at the cellular level. Fas (Apo-1/CD95) is a cell-surface receptor involved in cell death signaling. In this study we analyzed, by immunohistochemistry, the expression of Fas protein in a group of 68 NSCLC (non-small cell lung cancer) specimens. Statistical analyses did not show any significant relationship between Fas immunohistochemical expression and clinical parameters of lung cancer patients, except for nodal status. Our data do not support the use of Fas as a molecular prognostic role in NSCLC. On the other hand, considering the role of the Fas-pathway in chemotherapy-induced apoptosis, further studies are required to analyze the potential value of Fas expression in predicting the response of NSCLC patients to chemotherapy.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Neoplasias Pulmonares/metabolismo , Receptor fas/metabolismo , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Adenocarcinoma/cirurgia , Antígenos CD/metabolismo , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma Pulmonar de Células não Pequenas/cirurgia , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/cirurgia , Feminino , Humanos , Imuno-Histoquímica/métodos , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Estudos Retrospectivos , Análise de Sobrevida
7.
Anticancer Res ; 23(6D): 5173-9, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14981985

RESUMO

BACKGROUND: Liver toxicity can be observed during treatment with most chemotherapic agents, and represents one of the principal causes of dose reduction or chemotherapy delays. S-Adenosylmethionine (AdoMet) plays a critical role in the synthesis of polyamines and provides cysteine for the production of glutathione (GSH), the major endogenous hepatoprotective agent. Our study was aimed at assessing the protective effect of AdoMet supplementation in cancer chemotherapy-induced liver toxicity. PATIENTS AND METHODS: Fifty cancer patients who developed, for the first time, anticancer chemotherapy-induced liver toxicity were studied. Enrolled patients received oral AdoMet supplementation. RESULTS: AST, ALT and LDH levels recorded at the moment of the recognition of liver toxicity were significantly reduced after one week of AdoMet therapy (respectively p: 0.009, 0.0005 and 0.012). AST, ALT and LDH decrease was confirmed after two weeks of treatment. Furthermore, the effect on these enzyme levels persisted in the following chemotherapy courses, permitting our patients to perform the scheduled chemotherapy courses with a minimal number of dose reductions or administration delays. The efficacy of AdoMet supplementation was not influenced by the presence of liver metastases, and no appreciable side-effects were recognized. CONCLUSION: The results of our study clearly demonstrate a protective effect of AdoMet in cancer chemotherapy-induced liver toxicity. Further large phase III studies are required to assess the real clinical benefit associated with AdoMet supplementation.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Hepatopatias/prevenção & controle , S-Adenosilmetionina/uso terapêutico , Adulto , Idoso , Alanina Transaminase/metabolismo , Aspartato Aminotransferases/metabolismo , Doença Hepática Induzida por Substâncias e Drogas , Feminino , Humanos , L-Lactato Desidrogenase/metabolismo , Hepatopatias/enzimologia , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
8.
In Vivo ; 16(5): 333-6, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12494873

RESUMO

Apelin is an endogenous ligand of the human orphan receptor APJ (orphan G protein-coupled receptor). This peptide is produced through processing from the C-terminal portion in the pre-proprotein consisting of 77 amino acid residues and exists in multiple molecular forms. Although the main physiological functions of apelin have not been clarified yet, it has been demonstrated that apelin partially suppresses cytokine production from mouse spleen and, specifically, induces the promotion of extracellular acidification and inhibition of cAMP production in Chinese hamster ovary cells. Moreover, it is involved in the regulation of blood pressure and blood flow. In this study we have analyzed, by immunohistochemistry, apelin distribution in several human tissues, demonstrating that apelin has a widespread pattern of expression. These results seem to confirm that apelin functions widely in various tissues interacting with its specific receptor APJ.


Assuntos
Proteínas de Transporte/metabolismo , Especificidade de Órgãos , Apelina , Feminino , Humanos , Imuno-Histoquímica , Peptídeos e Proteínas de Sinalização Intercelular , Ligantes , Distribuição Tecidual
9.
In Vivo ; 16(5): 387-90, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12494880

RESUMO

A rare case of papillary pneumocytoma of the lung is reported. The immunohistochemical positivities for EMA, cytokeratin and TTF-l strongly support the hypothesis that the neoplastic cells are originated from type 2 pneumocytes. The tumour also presented areas displaying stroma of non-specific mixoid appearance, simulating a benign tumour of the salivary gland type. We propose that these mixoid areas constitute structures of metaplastic origin.


Assuntos
Adenoma Pleomorfo/patologia , Adenoma/patologia , Neoplasias Brônquicas/patologia , Adenoma/química , Adenoma/cirurgia , Idoso , Biomarcadores Tumorais/análise , Neoplasias Brônquicas/química , Neoplasias Brônquicas/cirurgia , Diagnóstico Diferencial , Humanos , Queratinas/análise , Masculino , Mucina-1/análise , Proteínas Nucleares/análise , Radiografia Torácica , Fator Nuclear 1 de Tireoide , Fatores de Transcrição/análise , Resultado do Tratamento
10.
Clin Cancer Res ; 8(12): 3850-6, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12473599

RESUMO

Altered expression of cell cycle regulators represents a frequent event in both small cell and non-small cell lung cancer (NSCLC). Despite several studies that reported involvement of tumor suppressor genes, such as p53 and pRb, in the development and progression of lung cancer, contrasting opinions exist about the prognostic role of this protein in this neoplasm. We developed an immunohistochemical assay suitable for the detection of pRb2/p130, the last discovered member of the retinoblastoma gene family, on formalin-fixed and paraffin-embedded sections. We evaluated the immunohistochemical expression of pRb2/p130 in 135 lung cancer specimens, and performed Western blot analysis in a subset of 30 corresponding tumor lysates. A high correlation between immunohistochemical data and Western blot results (P = 0.0004) was found. We statistically analyzed the relationship between overall survival (OS) time and pRb2/p130 expression according to the different histological types in 105 patients. We did not find any correlation between pRb2/p130 expression and OS in small cell lung cancers, whereas in NSCLCs a direct relationship between pRb2 and OS was found in both adenocarcinoma (P = 0.0002) and squamous cell carcinoma (P = 0.0002) histotypes. According to univariate analysis, pRb2/p130 was a prognostic factor of which the lost or reduced expression correlated with a shorter OS (P < 0.0000). At multivariate analysis, pRb2/p130 expression was an independent predictor of OS (P = 0.0001) when considered together with histotype. This study demonstrates for the first time the potential independent prognostic value of pRb2/p130 expression on formalin-fixed, paraffin-embedded sections from lung cancer patients. pRb2/p130 immunoreactivity can be used to predict OS in patients with NSCLC and, therefore, may represent a new prognostic marker.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma de Células Pequenas/metabolismo , Neoplasias Pulmonares/metabolismo , Fosfoproteínas/metabolismo , Proteínas , Proteína do Retinoblastoma/metabolismo , Western Blotting , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Carcinoma de Células Pequenas/patologia , Regulação para Baixo , Feminino , Humanos , Técnicas Imunoenzimáticas , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Proteína p130 Retinoblastoma-Like , Taxa de Sobrevida
11.
Anticancer Res ; 22(6C): 4039-43, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12553029

RESUMO

Cell adhesion is a basic count in inter- and intracellular communication and plays an important role in tumor progression. In this study, the expression of E-selectin, ICAM-1 and VCAM-1 were evaluated by means of immunohistochemistry in a group of 153 lung cancer specimens. E-selectin immunoreactivity was localized mostly on endothelial cell venules and capillaries with an average staining intensity of 75% of cells in the NSCLC, while in SCLC the intensity of the staining was 69%. The staining pattern for ICAM-1 reached an average intensity of 57%, in both NSCLC and SCLC. Finally, VCAM-1 immunoreactivity was detected only in NSCLC with an average intensity of 12% on endothelial cell venules and capillaries. This study provides a contribution towards the understanding of the basic mechanisms of cell adhesion in lung cancer progression.


Assuntos
Selectina E/biossíntese , Molécula 1 de Adesão Intercelular/biossíntese , Neoplasias Pulmonares/metabolismo , Molécula 1 de Adesão de Célula Vascular/biossíntese , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Western Blotting , Carcinoma de Células Pequenas/metabolismo , Carcinoma de Células Pequenas/patologia , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias
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