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J Biol Chem ; 272(18): 12189-94, 1997 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-9115292

RESUMO

Macrophage elastase (ME) was originally named when metal-dependent elastolytic activity was detected in conditioned media of murine macrophages. Subsequent cDNA cloning of the mouse and human enzyme demonstrated that ME is a distinct member of the matrix metalloproteinase family. To date, the catalytic parameters that describe the hydrolysis of elastin by ME have not been quantified and its activity against other matrix proteins have not been described. In this report, we have examined the action of purified recombinant human ME (rHME), produced in Escherichia coli, on elastin and other extracellular matrix proteins. On a molar basis, rHME is approximately 30% as active as human leukocyte elastase in solubilizing elastin. rHME also efficiently degrades alpha1-antitrypsin (alpha1-AT), the primary physiological inhibitor of human leukocyte elastase. In addition, rHME efficiently degrades fibronectin, laminin, entactin, type IV collagen, chondroitan sulfate, and heparan sulfate. These results suggest that HME may be required for macrophages to penetrate basement membranes and remodel injured tissue during inflammation. Moreover, abnormal expression of HME may contribute to destructive processes such as pulmonary emphysema and vascular aneurysm formation. To further understand the specificity of HME, the initial cleavage sites in alpha1-AT have been determined. In addition, the hydrolysis of a series of synthetic peptides with different P'1 residues has been determined. rHME can accept large and small amino acids at the P'1 site, but has a preference for leucine.


Assuntos
Proteínas da Matriz Extracelular/metabolismo , Macrófagos/enzimologia , Elastase Pancreática/metabolismo , Sequência de Aminoácidos , Animais , Células Cultivadas , Clonagem Molecular , Meios de Cultivo Condicionados , Escherichia coli , Proteínas da Matriz Extracelular/biossíntese , Proteínas da Matriz Extracelular/isolamento & purificação , Humanos , Hidrólise , Cinética , Leucócitos/enzimologia , Macrófagos/citologia , Camundongos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Especificidade por Substrato
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