[Vulvar vestibulitis syndrome as an important clinical problem]. / Zespól zapalenia przedsionka pochwy jako istotny problem kliniczny.

Clinical problems of etiology and therapy vulvovaginitis syndrome were shown.

[Epidemiology of the pregnant women risk groups according to Troszynski's criterion with special regard to risk factors of potentially environmental character]. / Epidemiologia grup ryzyka kobiet ciezarnych wedlug Troszynskiego ze szczególnym uwzglednieniem czynników ryzyka ciazy mogacych zalezec od srodowiska.

THE AIM: To evaluate the occurrence of specific increased risk groups of pregnancies against a background of the environment contamination in Rybnik. Environmental pregnancy risk factors were particularly taken into consideration. METHODS: Pregnant women giving birth in the Obstetrical Department in Rybnik in the years 1986-2000. Were study subject. The occurrence of factors dependent on the environment was the object of the inquiry. RESULTS AND CONCLUSIONS: The concentration of chemical substances in Rybnik in the years 1988-2000 revealed a declining tendency. Uncomplicated pregnancies were noticed in 50% of the cases. There was no evident correlation between professional activity and the occurrence of increased risk groups.

[Analysis of stillbirths in Rybnik in the years 1991-2000]. / Analiza ciaz obumarlych na terenie miasta Rybnika w latach 1991-2000.

153 cases of stillbirths, which took place in Gynecological & Obstetrical Department in Rybnik by 1991-2000 were analyzed. In one third of the cases women did not receive prenatal care. Most stillbirths were noticed in a birth weight range between 501-1000 g. The majority of parturients were unemployed and multiparous ones.

Immunohistochemical analysis of P-glycoprotein expression in breast cancer: clinical correlations.

Paraffin-embedded, formalin-fixed tissue (PEFFT) specimens from a subset of breast cancer patients were analyzed by immunohistochemistry to determine whether or not the presence of P-glycoprotein identified chemotherapy resistance. Two antibodies, C219 (monoclonal) and Ab1 (polyclonal), demonstrated appropriate immunostaining. Retrospectively and prospectively, P-glycoprotein expression was determined from PEFFT of 20 breast cancer biopsies (19 patients with locally advanced or metastatic disease). Immunohistochemical staining was graded for number of positive cells (N0 to N4) and intensity (I0 to I3). The immunostaining N and I of both antibodies were similar. There was no correlation between N, (P = 0.13) or I, (P = 0.67) and chemotherapy response or between N, (P = 0.63) or I, (P = 0.89) and survival. Five patients had residual cancer at repeat biopsy after systemic chemotherapy for locally advanced disease. These specimens had similar N and I as the primary cancer. This assay accurately identifies P-glycoprotein expression in PEFFT and revealed no correlation between expression and chemotherapy response or survival.

Cytometrically coherent transfer of receptor proteins on microporous membranes.

A new method (Freeze-Transfer) is described for performing high-resolution immunocytochemistry for soluble cell proteins on frozen sections of biological tissues that involves thaw-mounting frozen tissue sections directly onto the surface of nitrocellulose thin films instead of directly onto glass slides. This technically straight-forward change in methodology resulted in chromogenic immunocytochemical assays for Her-2 and EGF receptors that were 1-2 orders of magnitude more sensitive while still fully utilizing the diagnostic resolving power of light microscopy. The effects of membrane pore size and surface chemistry on the resolution and intensity of Her-2 signal suggest that the enhanced sensitivity of Freeze-Transfer was caused by the cytologically coherent transfer of target molecules normally lost from cut surfaces of cells mounted on nonporous glass during assay.