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2.
Sci Rep ; 10(1): 5109, 2020 03 20.
Artigo em Inglês | MEDLINE | ID: mdl-32198370

RESUMO

Splenic immune function was enhanced in diet-induced-obese (DIO) mice caused by Escherichia coli. The changes in spleen function on apoptosis were still unknown. Two hundred mice in groups Lean-E. coli and DIO-E. coli were intranasal instillation of E. coli. And another two hundred mice in groups Lean-PBS and DIO-PBS were given phosphate-buffered saline (PBS). Subsequently, spleen histology was analyzed. Then the rates of spleen cell (SC) apoptosis, and expression of the genes and proteins of Bcl-2, Bax, caspase-3 and caspase-9 were quantified in each group at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. The SC apoptosis rates of the DIO-E. coli groups were lower than those of the DIO-PBS groups at 12, 24 and 72 h (p < 0.05). Anti-apoptotic Bcl-2 expression gene and protein of the DIO-E. coli groups were higher than those of the DIO-PBS groups (p < 0.05). Gene expressions of pro-apoptotic Bax, caspase-3 and caspase-9 of the DIO-E. coli groups were lower than those of DIO-PBS groups at 12, 24 and 72 h (p < 0.05). The SC apoptosis rates of the Lean-E. coli groups were higher than those of the Lean- PBS groups at 12 h and 24 h (p < 0.05). Interestingly, the SC apoptosis rates in the DIO-E. coli groups were lower than those of the Lean-E. coli groups at 12 h (p < 0.05). In conclusion, our results suggested that the DIO mice presented stronger anti-apoptotic abilities than Lean mice in non-fatal acute pneumonia induced by E. coli infection, which is more conducive to protecting the spleen and improving the immune defense ability of the body.


Assuntos
Apoptose/imunologia , Infecções por Escherichia coli/imunologia , Escherichia coli/imunologia , Obesidade/imunologia , Pneumonia/imunologia , Baço/imunologia , Administração Intranasal , Animais , Caspase 3/biossíntese , Caspase 9/biossíntese , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/mortalidade , Macrófagos/imunologia , Masculino , Megacariócitos/imunologia , Camundongos , Camundongos Obesos , Neutrófilos/imunologia , Pneumonia/microbiologia , Pneumonia/mortalidade , Proteínas Proto-Oncogênicas c-bcl-2/biossíntese , Baço/citologia , Proteína X Associada a bcl-2/biossíntese
3.
J Immunol Res ; 2020: 5948256, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32104715

RESUMO

Obese mice exhibited more lymphocytes in the bronchoalveolar lavage fluid and milder lung injury after Escherichia coli (E. coli) infection. However, it remained unclear whether the spleen contributed to the effect of obese mice with infection. The study was purposed to reveal the histopathological changes of the spleen caused by oxidative stress and inflammation in diet-induced obesity (DIO) mice challenged by Escherichia coli. After infection, the spleen tissues were obtained in normal and DIO mice at 0 h (uninfected), 12 h, 24 h, and 72 h postinfection. Results revealed that DIO mice have higher contents of resistin, TNF-α, IL-6, and IL-1ß in the spleen than normal mice and lower concentrations of GSH-Px, SOD, and CAT and higher MDA than normal mice. After an intranasal drip of E. coli, the activities of GSH-Px, SOD, and CAT in the DIO mice were elevated and the content of MDA declined. The activities of SOD and CAT in the normal mice declined, and the content of MDA was elevated. Moreover, the contents of TNF-α, IL-6, and IL-1ß in the spleen declined in DIO mice at 24 and 72 h, although the contents of leptin, resistin, TNF-α, IL-6, and IL-1ß were elevated at 12 h. The contents of resistin, TNF-α, IL-6, and IL-1ß were elevated in normal mice at 12 and 24 h. Those results indicated that obesity elevated splenic oxidation and inflammatory levels, but it enhanced antioxidant capacity and reduced cytokine levels of the spleen in mice to resist splenic injury after an intranasal drip of E. coli.


Assuntos
Antioxidantes/metabolismo , Citocinas/metabolismo , Infecções por Escherichia coli/metabolismo , Infecções por Escherichia coli/microbiologia , Escherichia coli , Obesidade/metabolismo , Baço/metabolismo , Baço/microbiologia , Animais , Biomarcadores , Biópsia , Modelos Animais de Doenças , Infecções por Escherichia coli/diagnóstico , Fibrose , Camundongos , Obesidade/etiologia , Estresse Oxidativo , Baço/patologia , Avaliação de Sintomas
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