Transcriptomic data of BT549 triple negative breast cancer cells treated with 20 µM NU7441, a DNA-dependent kinase inhibitor.

DNA-dependent protein kinase catalytic subunit (DNA-PK) is a multifunctional serine­threonine protein kinase that plays roles in non-homologous end joining of DNA repair in cells. NU7441 is a specific DNA-PKcs inhibitor. We investigated the effects of NU7441 on the transcriptome of BT549 triple negative breast cancer cells. Total RNA extracted from NU7441-treated or control BT549 cells was processed for preparation of sequencing libraries. Assessment of read quality was performed using fastqc tool. Trimming and filtering low-quality reads were performed using fastp. Reads were aligned by hisat2. SAM files were converted to BAM files using Samtools. The gene differential expression analysis, Gene Ontology (GO) analysis and KEGG pathway analysis were performed. After NU7441 treatment, total number of 2045 differential genes were selected according to |log2(FoldChange)| >= 1 & padj<= 0.05, among which 1365 genes were down-regulated and 680 genes were up-regulated. The differential expression genes in pattern recognition receptors (PRRs) immune responses signals, including NOD-like receptor signaling, Toll-like receptor signaling, RIG-I-like receptor signaling and cytosolic DNA-sensing pathways were noted in this paper.

One-Pot Synthesis of MOF@MOF: Structural Incompatibility Leads to Core-Shell Structure and Adaptability Control Makes the Sequence.

Core-shell metal-organic frameworks (MOF@MOF) are promising materials with sophisticated structures that cannot only enhance the properties of MOFs but also endow them with new functions. The growth of isotopic lcore-shell MOFs is mostly limited to inconvenient stepwise seeding strategies with strict requirements, and by far one-pot synthesis is still of great challenge due to the interference of different components. Through two pairs of isoreticular MOFs, it reveals that the structural incompatibility is a prerequisite for the formation of MOFs@MOFs by one-pot synthesis, as illustrated by PMOF-3@HHU-9. It further unveils that the adaptability of the shell-MOF is a more key factor for nucleation kinetic control. MOFs with flexible linkers has comparably slower nucleation than MOFs with rigid linkers (forming PMOF-3@NJU-Bai21), and structural-flexible MOFs built by flexible linkers show the lowest nucleation and the most adaptability (affording NJU-Bai21@HHU-9). This degree of adaptability variation controls the sequence and further facilitates the synthesis of a first triple-layered core-shell MOF (PMOF-3@NJU-Bai21@HHU-9) by one-pot synthesis. The insight gained from this study will aid in the rational design and synthesis of other multi-shelled structures by one-pot synthesis and the further expansion of their applications.