Assuntos
Hepatite A/complicações , Alanina Transaminase/sangue , Criança , Pré-Escolar , Feminino , Hepatite C/complicações , Humanos , MasculinoRESUMO
Commercial bulk products and pharmaceutical drug formulations of aminoglycoside antibiotics obtained by fermentation (kanamycin, gentamicin, sisomicin and tobramycin) or by synthesis (amikacin) were analysed with high-performance liquid chromatography on a C8 reversed-phase column. The method is based on a pre-column derivatization of the aminoglycosides with a 2,4,6-trinitrobenzenesulphonic acid reagent and UV detection (350 nm). The quantitative determination was carried out vs. an external standard; both peak heights and areas were used. A gentamicin mixture was separated into five or four components, depending on the column used. Amikacin was separated from its possible regioisomers and kanamycin A was easily separated from its minor components B and C.
Assuntos
Antibacterianos/análise , Amicacina/análise , Aminoglicosídeos/análise , Aminoglicosídeos/isolamento & purificação , Antibacterianos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Gentamicinas/análise , Espectrofotometria UltravioletaRESUMO
Antibodies to HTLV-III have been investigated in 118 multitransfused beta-thalassemia patients. Thirteen patients (11.01%) were found to be positive; 3 of these 13 showed clinical and immunological signs of the lymphadenopathy syndrome. A retrospective study carried out on 65 sera has shown that at least 6 patients were negative 3 years before the present investigation. This is the first extensive study on HTLV-III infection in multitransfused beta-thalassemics. It suggests that these patients are at risk for the acquired immune deficiency syndrome and related diseases.
