RESUMO
The proportion of symmetric and asymmetric IgG molecules was studied in 10 mothers at delivery. IgG was obtained from peripheral blood and placental blood sera and by elution at 4 M KCl from placenta cell membranes. The percentage of symmetric and asymmetric molecules was determined in the IgG and in their corresponding F(ab')2 fragments by absorption to Con A-Sepharose. The presence of antipaternal antibodies was investigated by IIF and MC tests using paternal lymphocytes. The average percentage of asymetric IgG molecules in the sera was 24.4, which is about double the value of that found in normal subjects. In the IgG eluted from the placenta, the proportion of asymmetric IgG was much higher, averaging 44.4%. Antipaternal antibodies were detected in 5 mothers by IIF and MC and in two mothers only by IIF. In three mothers no antibodies could be detected. It was found that the concentration of antipaternal antibodies was about three times higher in the asymmetric IgG fraction than in the summetric one. Considering the percentage of asymmetric IgG molecules with antipaternal antigen specificity eluted from placenta and the possibility that they function as blocking antibodies, their participation in fetal protection is suggested.
Assuntos
Imunoglobulina G/análise , Placenta/imunologia , Gravidez/imunologia , Testes Imunológicos de Citotoxicidade , Eletroforese em Gel de Poliacrilamida , Feminino , Imunofluorescência , Humanos , Fragmentos Fab das Imunoglobulinas/análise , Fragmentos Fab das Imunoglobulinas/imunologia , Imunoglobulina G/isolamento & purificação , Gravidez/sangueRESUMO
In the present study Latin-American celiac disease patients were analyzed for the frequency of certain HLA class II restriction fragment length polymorphisms in order to investigate whether they exhibited the normal associated alleles or showed unusual class II variants. A DPB/RsaI 4.0-kb fragment that was shown to be significantly increased among North Americans celiac disease patients of the DR3,DQw2 haplotype was found with similar frequency in Latin-American control and celiac disease individuals. A DPA/BglII 3.7-kb fragment previously shown to be increased among British celiac disease patients was also present with similar frequency among Latin-American control and celiac disease individuals. These results show that the frequency of the HLA-DP region-derived restriction fragment length polymorphisms linked to celiac disease differs between Caucasian populations of different ethnic backgrounds (Anglo-Saxon and Latin-American). On the other hand, DNA samples from 13 patients and 14 controls bearing the DR5/DR7 phenotype (which is significantly associated with celiac disease in Latin populations) were investigated for the presence of particular restriction fragment length polymorphisms disproportionally present in celiac disease individuals. No significant differences were found between controls and patients when the DNA was analyzed with 10 different restriction enzymes and probes for DRB, DQA, DQB, and DPB HLA class II sequences.
Assuntos
Doença Celíaca/genética , Genes MHC da Classe II , Polimorfismo de Fragmento de Restrição , Alelos , Doença Celíaca/imunologia , Criança , DNA/análise , Feminino , Antígenos HLA-DP/genética , Humanos , América Latina , Masculino , Fenótipo , População Branca/genéticaRESUMO
In this report we discuss the results of the association of chronic active hepatitis (B virus) and coeliac disease with HLA class I and class II antigens, in patients of Latin American Caucasian origin. Evidence is presented showing that the alleles involved differ from those reported in other Caucasian populations of different ethnic background. Differences were observed both at the serology and at the DNA (RFLP) level. The relevance of these findings regarding the clinical implications as well as the molecular mechanisms involved in the associations are discussed.
Assuntos
Doença Celíaca/genética , Antígenos HLA/genética , Hepatite Crônica/genética , Polimorfismo de Fragmento de Restrição , Argentina , Doença Celíaca/etnologia , Doença Celíaca/imunologia , Frequência do Gene , Antígeno HLA-B35/genética , Hepatite Crônica/etnologia , Hepatite Crônica/imunologia , HumanosRESUMO
En este trabajo se describen los resultados de dos estudios de asociación de patologías a determinados alelos de clase I y clase II del sistema HLA en pacientes caucásicos argentinos: la hepatitis crónica activa (virus B) y la enfermedad celíaca. Se presentan evidencias que muestran para ambas patologías que los alelos HLA involucrados no son los mismos que los hallados para otros grupos éticos. Estas diferencias residen tanto a nivel serológico como a nivel del DNA (evaluable por RFLP). Estos hallazgos son relevantes tanto en lo referente a las aplicaciones clínicas de las asociaciones descriptas (por ejemplo la tipificación de hermanos o hijos de pacientes celíacos para identificar a los portadores de los alelos de riesgo), como así también para invetigar las bases moleculares de las maismas (AU)
Assuntos
Humanos , Antígenos HLA/genética , Polimorfismo de Fragmento de Restrição , Hepatite Crônica/genética , Doença Celíaca/genética , Argentina , Frequência do Gene , Antígenos HLA/genética , Hepatite Crônica/imunologia , Doença Celíaca/imunologiaRESUMO
En este trabajo se describen los resultados de dos estudios de asociación de patologías a determinados alelos de clase I y clase II del sistema HLA en pacientes caucásicos argentinos: la hepatitis crónica activa (virus B) y la enfermedad celíaca. Se presentan evidencias que muestran para ambas patologías que los alelos HLA involucrados no son los mismos que los hallados para otros grupos éticos. Estas diferencias residen tanto a nivel serológico como a nivel del DNA (evaluable por RFLP). Estos hallazgos son relevantes tanto en lo referente a las aplicaciones clínicas de las asociaciones descriptas (por ejemplo la tipificación de hermanos o hijos de pacientes celíacos para identificar a los portadores de los alelos de riesgo), como así también para invetigar las bases moleculares de las maismas
Assuntos
Humanos , Antígenos HLA/genética , Doença Celíaca/genética , Hepatite Crônica/genética , Polimorfismo de Fragmento de Restrição , Antígenos HLA/genética , Argentina , Doença Celíaca/imunologia , Frequência do Gene , Hepatite Crônica/imunologiaRESUMO
In this report we discuss the results of the association of chronic active hepatitis (B virus) and coeliac disease with HLA class I and class II antigens, in patients of Latin American Caucasian origin. Evidence is presented showing that the alleles involved differ from those reported in other Caucasian populations of different ethnic background. Differences were observed both at the serology and at the DNA (RFLP) level. The relevance of these findings regarding the clinical implications as well as the molecular mechanisms involved in the associations are discussed.
