Combing remote sensing information entropy and machine learning for ecological environment assessment of Hefei-Nanjing-Hangzhou region, China.

Urban ecological environment is the basis of citizens' survival and development. A rapid and objective urban ecological environment assessment (UEEA) plays an important role in the urban sustainable development and environment protection. This study established an improved urban ecological comfort index (UECIIMP), which is based on our previous UECI and fully composed of four remote sensing indicators: normalized difference vegetation index (NDVI), normalized difference built-up index (NDBI), land surface temperature (LST), and aerosol optical depth (AOD), representing the greenness, dryness, heat, and atmospheric turbidity, respectively. Combining the entropy method and random forest (RF) algorithm, the weights of four indicators were calculated. To improve the accuracy of UECIIMP, the gap-filled quarterly mean results of each indicator with 30m resolution were obtained using the harmonic analysis of time series (HANTS) method and spatial-temporal information fusion based on non-local means filter (STNLFFM). UECIIMP was applied to the Hefei-Nanjing-Hangzhou Region to explore its spatiotemporal changes and response characteristics. Results show that the weights of UECIIMP fluctuate slightly (within 10%) before and after sensitivity analysis, with good stability and reliability. UECIIMP in Hangzhou > Hefei ≈ Nanjing, spring ≈ autumn > summer â‰« winter. From 2009 to 2019, UECIIMP has improved in all 33 districts of the Hefei-Nanjing-Hangzhou Region. The significant improvement of UECIIMP in 2014-2019 is 4.3 times than that in 2009-2014. The correlation between UECIIMP and economic index indicates that economic development has a positive impact on the urban ecological environment. The significant degradation of UECIIMP in the urban expansion area demonstrates a negative impact on the local environment from urban expansion.

[Temporal and Spatial Variations in Black Carbon Aerosol in Different Atmospheric Background Stations in China from 2006 to 2020].

Black carbon (BC) aerosol emissions are complex and have important environmental and meteorological effects. In China, the temporal and spatial variations in BC in different atmospheric environmental conditions need to be fully understood. Based on the long-term observational BC data in seven atmospheric background stations in China from 2006 to 2020, combined with meteorological data, emission source data, enhanced vegetation index (EVI) data, and aerosol optical depth (AOD) data, we comprehensively analyzed the characteristics of temporal and spatial variations, long-term evolution, and influencing factors of BC in China. The results showed that the BC and AOD values of different atmospheric environments in China were quite different, and BC positively contributed to AOD. The spatial distribution was high in the east and low in the west owing to the differences in emission sources and meteorological conditions. The ρ(BC) and AOD values were higher to the east of the "Hu Huanyong" line, such as at the Mt. Longfeng, Shangdianzi, Lin'an, and Jinsha stations, where the average values were (1699±2213)-(3392±2131) ng·m-3 and 0.36±0.32-0.72±0.37, respectively. These values were lower to the west of the "Hu Huanyong" line, such as at the Akedala, Mt. Waliguan, and Shangri-La stations, where the average values were (287±226)-(398±308) ng·m-3 and 0.20±0.13-0.22±0.19, respectively. The interannual variability in BC included differences between different atmospheric background stations, which could be divided into four categories:low interannual variability, such as at the Akedala station; an initial increase followed by a decrease and subsequent stabilization, such as at the Mt. Waliguan station; an initial decrease followed by stabilization, such as at the Shangri-La station; and an initial stabilization followed by a decrease, such as at the Mt. Longfeng, Shangdianzi, Jinsha, and Lin'an stations. Seasonal variations in BC included differences in different atmospheric background stations. The BC mass concentrations were lowest in autumn and higher in winter and spring west of the "Hu Huanyong" line and were highest in winter and lowest in summer east of the "Hu Huanyong" line. BC contributed to the AOD being larger in all stations in the spring and summer and contributed less at the stations west of the "Hu Huanyong" line in autumn and the stations east of the "Hu Huanyong" line in winter. The diurnal variations in BC were mainly bimodally distributed in the different atmospheric background stations, but the peak times varied in different stations and seasons.

A long-term and comprehensive assessment of the urbanization-induced impacts on vegetation net primary productivity.

Urbanization not only directly alters the regional ecosystem net primary productivity (NPP) through land-cover replacement, but it is also accompanied by huge indirect impacts due to the associated climate change and anthropogenic activities. However, to date, limited efforts have been made to quantitatively separate the two types of urbanization impacts, and the continuous variations over a long-time span are not well understood. In this study, both the long-term direct and indirect impacts of urbanization on NPP were established and analyzed based on multi-source remote sensing data, taking the city of Kunming in China as a case study area. The results indicated that the intense urbanization process has led to a continuous decrease in NPP from 1990 to 2014, due to the direct impact of land-cover replacement. Nevertheless, the urbanization has also resulted in an apparently positive indirect impact on NPP, which has offset about 30% of the direct impact in recent years. The increasing trend of the indirect impact was found to be higher than the NPP trend in the surrounding forest areas, which proves that vegetation growth has been promoted by the urban environment. The indirect impact has also shown great spatial and temporal heterogeneity, with generally higher values in the old city area and winter season. This can mostly be attributed to the distribution of temperature, i.e., the urban heat island effect, which has shown a significantly positive correlation with the indirect impact. However, the correlations between NPP and climatic factors were found to be completely different, which confirmed the need to separate the direct and indirect impacts. Overall, this study has demonstrated that urbanization has reduced the total NPP over the region, but has promoted some vegetation growth, and the knowledge of the indirect impact will help to support urban greening planning.

Analyses of deep mammalian sequence alignments and constraint predictions for 1% of the human genome.

A key component of the ongoing ENCODE project involves rigorous comparative sequence analyses for the initially targeted 1% of the human genome. Here, we present orthologous sequence generation, alignment, and evolutionary constraint analyses of 23 mammalian species for all ENCODE targets. Alignments were generated using four different methods; comparisons of these methods reveal large-scale consistency but substantial differences in terms of small genomic rearrangements, sensitivity (sequence coverage), and specificity (alignment accuracy). We describe the quantitative and qualitative trade-offs concomitant with alignment method choice and the levels of technical error that need to be accounted for in applications that require multisequence alignments. Using the generated alignments, we identified constrained regions using three different methods. While the different constraint-detecting methods are in general agreement, there are important discrepancies relating to both the underlying alignments and the specific algorithms. However, by integrating the results across the alignments and constraint-detecting methods, we produced constraint annotations that were found to be robust based on multiple independent measures. Analyses of these annotations illustrate that most classes of experimentally annotated functional elements are enriched for constrained sequences; however, large portions of each class (with the exception of protein-coding sequences) do not overlap constrained regions. The latter elements might not be under primary sequence constraint, might not be constrained across all mammals, or might have expendable molecular functions. Conversely, 40% of the constrained sequences do not overlap any of the functional elements that have been experimentally identified. Together, these findings demonstrate and quantify how many genomic functional elements await basic molecular characterization.

An intermediate grade of finished genomic sequence suitable for comparative analyses.

Although the cost of generating draft-quality genomic sequence continues to decline, refining that sequence by the process of "sequence finishing" remains expensive. Near-perfect finished sequence is an appropriate goal for the human genome and a small set of reference genomes; however, such a high-quality product cannot be cost-justified for large numbers of additional genomes, at least for the foreseeable future. Here we describe the generation and quality of an intermediate grade of finished genomic sequence (termed comparative-grade finished sequence), which is tailored for use in multispecies sequence comparisons. Our analyses indicate that this sequence is very high quality (with the residual gaps and errors mostly falling within repetitive elements) and reflects 99% of the total sequence. Importantly, comparative-grade sequence finishing requires approximately 40-fold less reagents and approximately 10-fold less personnel effort compared to the generation of near-perfect finished sequence, such as that produced for the human genome. Although applied here to finishing sequence derived from individual bacterial artificial chromosome (BAC) clones, one could envision establishing routines for refining sequences emanating from whole-genome shotgun sequencing projects to a similar quality level. Our experience to date demonstrates that comparative-grade sequence finishing represents a practical and affordable option for sequence refinement en route to comparative analyses.

[Descriptions and microscopy identification of Ephemerantha fimbriatum].

In this paper, the descriptions and microscopy characters of Ephemerantha fimbriatum were reported.