Multi-functional composite dressings with sustained release of MSC-SLP and anti-adhesion property for accelerating wound healing.

Exudate management is of significant clinical value for the treatment of acute wound. Various wound dressings have been developed to restore the function of injured tissues and promote wound healing, but proper exploiting the healing factors inside exudate and achieving anti-adhesion wound care remains a challenge. Herein, we present a novel multi-functional composite dressing (MCD) by coupling supernatant lyophilized powder of mesenchymal stem cells (MSC-SLP) with a sandwich-structured wound dressing (SWD). The developed MCDs demonstrated unique unidirectional drainage capability, stable anti-adhesion characteristics, and improved wound healing performance. The designed SWD with both superhydrophobic inner surface and liquid-absorption ability of mid layer enables the dressings exhibit desired anti-adhesion property to neoformative granulation tissues, favorable shielding effect to exogenous bacteria, as well as appropriate exudate-retaining capability and unidirectional exudate-absorption property. The introduction of MSC-SLP in SWD was demonstrated to further improve wound healing quality. Compared to medical gauze, the synergic effect of SWD and MSC-SLP significantly accelerates wound healing rate by over 30%, avoids tissue avulsion when changing dressings, and produces a flat-smooth closure surface. More importantly, the wound treated with MCDs presents more skin accessory organs and blood vessels in regenerated tissues than other groups. In vivo/vitro biocompatibility evaluations indicated little toxicity, demonstrating the biosecurity of the developed dressings. The proposed method offers great potential in clinical applications particularly for chronic wound treatments.

Ultra-Large Scale Stitchless AFM: Advancing Nanoscale Characterization and Manipulation with Zero Stitching Error and High Throughput.

The atomic force microscopy (AFM) is an important tool capable of characterization, measurement, and manipulation at the nanoscale with a vertical resolution of less than 0.1 nm. However, the conventional AFMs' scanning range is around 100 µm, which limits their capability for processing cross-scale samples. In this study, it proposes a novel approach to overcome this limitation with an ultra-large scale stitchless AFM (ULSS-AFM) that allows for the high-throughput characterization of an area of up to 1 × 1 mm2 through a synergistic integration with a compliant nano-manipulator (CNM). Specifically, the compact CNM provides planar motion with nanoscale precision and millimeter range for the sample, while the probe of the ULSS-AFM interacts with the sample. Experimental results show that the proposed ULSS-AFM performs effectively in different scanning ranges under various scanning modes, resolutions, and frequencies. Compared with the conventional AFMs, the approach enables high-throughput characterization of ultra-large scale samples without stitching or bow errors, expanding the scanning area of conventional AFMs by two orders of magnitude. This advancement opens up important avenues for cross-scale scientific research and industrial applications in nano- and microscale.

Engineered Sandwich-Structured Composite Wound Dressings with Unidirectional Drainage and Anti-Adhesion Supporting Accelerated Wound Healing.

Proper management of exudate is of great clinical value for reducing wound infection and promoting wound healing, thus various dressings have been studied to address this widespread medical challenge. Herein, a novel sandwich-structured composite wound dressing (SCWD), integrating of a superlyophobic (SLO) polydimethylsiloxane (PDMS) layer, a superlyophilic gauze layer, and a lyophobic PDMS layer is presented, with particular unidirectional droplet drainage and stable anti-adhesion capabilities, which realizes effective management of wound exudate and provides a favorable environment for wound healing. Thanks to the stable SLO property on the PDMS surface with hierarchical micro/nanostructures, the continuously accumulated wound exudate at the interface between dressing and wound surface is gradually deformed, eventually passing through SLO PDMS layer through milli-scale channels and being absorbed by gauze layer. Experimental results show that the application of SCWD can significantly reduce the occurrence of wound infection, avoid the tearing of wound tissues when replacing dressings, and accelerate wound healing by ≈20%. The combination of SCWD and lyophilized powders of stem cells supernatant (LPSCS) is verified to better accelerate the healing process. The proposed method offers great potential in clinical applications, particularly for acute trauma wound treatments.

Laser Joining of Continuous Carbon Fiber-Reinforced PEEK and Titanium Alloy with High Strength.

The generation of high-performance heterojunctions between high-strength resin matrix composites and metals is of great significance for lightweight applications in fields such as aerospace and automobile engineering. Herein, we explored the feasibility of employing a laser joining process to achieve high-strength heterojunctions between continuous carbon fiber-reinforced PEEK (CCF30/PEEK) composites and titanium alloy (Ti6Al4V). A joint strength of over 50 MPa was achieved through constructing mechanical interlocking structures between CCF30/PEEK and Ti6Al4V. Tensile tests revealed that the fracture of joints was mainly ascribed to the detachment of carbon fibers from the resin matrix and the breakage of carbon fibers. The structures with different orientations and dimensions were confirmed to significantly influence the formation of interlocking structures near the joining interface and the resultant fracture strength of joints. It is believed that the results presented in this study provide a strong foundation for the production of high-performance heterojunctions.

Artificial neural network for cytocompatibility and antibacterial enhancement induced by femtosecond laser micro/nano structures.

The failure of orthopedic and dental implants is mainly caused by biomaterial-associated infections and poor osseointegration. Surface modification of biomedical materials plays a significant role in enhancing osseointegration and anti-bacterial infection. In this work, a non-linear relationship between the micro/nano surface structures and the femtosecond laser processing parameters was successfully established based on an artificial neural network. Then a controllable functional surface with silver nanoparticles (AgNPs) to was produced to improve the cytocompatibility and antibacterial properties of biomedical titanium alloy. The surface topography, wettability, and Ag+ release were carefully investigated. The effects of these characteristics on antibacterial activity and cytocompatibilty were also evaluated. Results show that the prepared surface is hydrophobic, which can prevent the burst release of Ag+ in the initial stage. The prepared surface also shows both good cytocompatibility toward the murine calvarial preosteoblasts MC3T3-E1 cells (derived from Mus musculus (mouse) calvaria) and good antibacterial effects against Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria, which is caused by the combined effect of appropriate micro/nano-structured feature and reasonable Ag+ release rate. We do not only clarify the antibacterial mechanism but also demonstrate the possibility of balancing the antibacterial and osteointegration-promoting properties by micro/nano-structures. The reported method offers an effective strategy for the patterned surface modification of implants.

Robust Heterojunctions of Metallic Alloy and Carbon Fiber-Reinforced Composite Induced by Laser Processing.

The development of heterojunctions with a strong bonding interface between metals and non-metals has attracted much attention owing to their great potential for use in lightweight structures. Laser joining technology, which emerged as a fast and reliable method, has proven its feasibility and unique advantages in joining metal to polymer matrix composites. Herein, an optimized laser joining configuration has been employed to realize high-quality joining of titanium alloy and carbon fiber-reinforced composite. Cross-sectional microstructures of laser-produced joints reveal that micro-bubbles near the interface have been effectively suppressed and eliminated due to the continual clamping pressure applied to the joined area during the joining process. Tensile tests suggest that the joint strength increases with structure density on a titanium alloy surface, and the greatest fracture strength of joints reaches more than 60 MPa even after experiencing a high-low temperature alternating aging test. For higher structure density (>95%), the joints fail by the fracture of parent plastics near the joined area due to the tensile-loading-induced peel stress at the edges of the overlap region. Otherwise, the joints fail by interfacial shear fracture with breakage when the structure density is lower than 91.5%. The obtained high-performance heterojunctions show great potential in the aerospace and automotive fields.

Enhancing SERS detection on a biocompatible metallic substrate for diabetes diagnosing.

A method to realize surface-enhanced Raman spectroscopy (SERS) at a titanium alloy substrate for glucose detection has been experimentally demonstrated. A silver-coated laser-induced periodic surface structure (LIPSS) was prepared via femtosecond laser micro-processing. The low detection limit of glucose is 10-7mol/L and, a good linear relationship between the glucose concentration and Raman intensity is found in the range between 1×10-7 and 1×10-3mol/L. Moreover, we investigate SERS detection for glucose sensing in human urine samples, while the results are in good agreement with clinical results. The Letter provides a facile method for producing a structure-controlled SERS substrate to realize glucose detection, which is promising for long-term in vivo diagnostics.

Laser Grinding of Single-Crystal Silicon Wafer for Surface Finishing and Electrical Properties.

In this paper, we first report the laser grinding method for a single-crystal silicon wafer machined by diamond sawing. 3D laser scanning confocal microscope (LSCM), X-ray diffraction (XRD), scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), laser micro-Raman spectroscopy were utilized to characterize the surface quality of laser-grinded Si. Results show that SiO2 layer derived from mechanical machining process has been efficiently removed after laser grinding. Surface roughness Ra has been reduced from original 400 nm to 75 nm. No obvious damages such as micro-cracks or micro-holes have been observed at the laser-grinded surface. In addition, laser grinding causes little effect on the resistivity of single-crystal silicon wafer. The insights obtained in this study provide a facile method for laser grinding silicon wafer to realize highly efficient grinding on demand.

In vitro evaluation of ultrafast laser drilling large-size holes on sheepshank bone.

Bone drilling has been widely used in medical surgeries such as repair and fixation in orthopedics. Traditional drilling method using drill-bits inevitably causes significant thermal and mechanical trauma in the adjacent bone tissues. This paper demonstrates the feasibility of femtosecond laser drilling in vitro large-size holes on the sheepshank bone with high efficiency and minimal collateral damage. A Yb:KGW femtosecond laser was utilized to drill millimeter-scale holes on the bone under different cooling conditions including gas- and water-assisted processes. Scanning electron microscopy, confocal laser scanning microscopy and infrared thermographic imaging system were used to investigate the residual debris, removal rate, bone temperature variation and hole morphology. Histological examination, Fourier transform infrared spectroscopy and Raman spectroscopy were employed to study thermal damage. Results show that a 4 mm hole with smooth and clean surface was successfully drilled on the bone, and the highest removal rate of 0.99 mm3/s was achieved, which was twenty times higher than the previous study of 0.05 mm3/s. Moreover, bone and bone marrow were distinguished by real-time monitoring system during laser drilling. This work demonstrates the potential for clinical applications using an ultrafast laser to produce crack-free large-size bone holes.

One-Step Fabrication of Robust Superhydrophobic Steel Surfaces with Mechanical Durability, Thermal Stability, and Anti-icing Function.

Superhydrophobic metallic materials have drawn broad research interest because of promising applications in various fields. The mechanical stability of superhydrophobic surfaces is currently a major concern limiting their practical applications. Herein, we developed a simple method to fabricate robust superhydrophobic surfaces on stainless steel via direct ultrafast laser microprocessing. Of note is that the fabricated superhydrophobic surfaces can withstand mechanical abrasion against an 800 grit SiC sandpaper for 2.3 m at an applied pressure of 5.5 kPa without losing superhydrophobicity. It is proposed that the robust superhydrophobicity may be attributable to the formation of unique hierarchical micro-/nanostructures and a nonpolar carbon layer on the surface. The hierarchical structures are composed of laser-created micropillars and ablation-induced nanoparticles. The fabricated surfaces exhibit good thermal stability and still show superhydrophobicity after thermal treatment at 100 °C for 120 min, which is related to the inorganic nature of metallic materials. An excellent anti-icing property is achieved on the fabricated surfaces with the water droplets on it retaining the liquid state for over 500 min at -8.5 ± 0.5 °C, which benefits from the obtained superhydrophobicity, based on classic nucleation theory and the heat transfer between the rough solid surface and water droplet. We envision that the presented method provides a facile and effective route to fabricate large-area superhydrophobic surfaces with robust mechanical stability and excellent anti-icing property.

Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence Scattering.

The integration of surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF) has attracted increasing interest and is highly probable to improve the sensitivity and reproducibility of spectroscopic investigations in biomedical fields. In this work, dual-mode SERS and SEF hierarchical structures have been developed on a single bio-metallic substrate. The hierarchical structure was composed of micro-grooves, nano-particles, and nano-ripples. The crystal violet was selected as reporter molecule and both the intensity of Raman and fluorescence signals were enhanced because of the dual-mode SERS-SEF phenomena with enhancement factors (EFs) of 7.85 × 105 and 14.32, respectively. The Raman and fluorescence signals also exhibited good uniformity with the relative standard deviation value of 2.46% and 5.15%, respectively. Moreover, the substrate exhibited high sensitivity with the limits of detection (LOD) as low as 1 × 10-11 mol/L using Raman spectroscopy and 1 × 10-10 mol/L by fluorescence spectroscopy. The combined effect of surface plasmon resonance and "hot spots" induced by the hierarchical laser induced periodical surface structures (LIPSS) was mainly contributed to the enhancement of Raman and fluorescence signal. We propose that the integration of SERS and SEF in a single bio-metallic substrate is promising to improve the sensitivity and reproducibility of detection in biomedical investigations.

Enhancement of Heat Dissipation by Laser Micro Structuring for LED Module.

Optimization for heat dissipation plays a significant role in energy saving and high-efficiency utilizing of integrated electronics. In this paper, we present a study of micro structuring on polymer-based flexible substrate coupled with aluminum-alloy heat sink. The heat dissipation performance was investigated by temperature evolution of a heat sink under natural convection by infrared (IR) camera, and results showed that the heat dissipation enhancement could be up to 25%. Moreover, the heat dissipation performance of a typical heat sink in terms of light-emitting diode (LED) hip was investigated via both thermal transient measurement and the finite element analysis (FEA). The maximum LED chip temperature of the laser-textured heat sink was approximately 22.4% lower than that of the as-received heat sink. We propose that these properties accompanied with the simplicity of fabrication make laser surface texturing a promising candidate for on-chip thermal management applications in electronics.

Enhancement of pulsed laser ablation in environmentally friendly liquid.

Enhancement of pulsed laser ablation can be achieved in acetic acid as an environmentally friendly liquid. This paper evaluates microholes and textured features induced by a nanosecond pulsed laser under different processing circumstances. The microholes are fabricated by laser drilling in acetic acid and found to be 100% deeper than in air. The textured features achieved in the liquid demonstrate a higher content of Copper and a lower content of Oxygen. The improvement of laser ablation efficiency in the liquid is attributed to the strong confinement of plasma plume accompanying with shockwave and cavitation bubbles. Meanwhile, the laser enhanced chemical etching by the weak acid plays a critical role.

Remediation of petroleum-contaminated soil after composting by sequential treatment with Fenton-like oxidation and biodegradation.

A laboratory study was conducted to enhance removal of residual contaminants after composting in a highly petroleum-contaminated soil by combining Fenton-like pretreatment with biodegradation. The contaminants were characterized by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) during soil treatment. The optimum molar ratio of H(2)O(2) and Fe(3+) was 300/1 determined in batch experiments. At the end of Fenton-like treatment, total dichloromethane-extractable organics (TEO) decreased from 32,400 to 21,800 mg kg(-1) soil, and the toxicity of soil was reduced greatly in the preoxidation process. A significant loss of the number of soil microorganisms was observed in the Fenton-like reaction. During the microbial treatment period, 50.6% of TEO was destroyed. Numerous varieties of polar compounds containing nitrogen and oxygen were identified by FT-ICR MS. The number of compounds containing two oxygen atoms dropped from 604 to 163 during Fenton-like oxidation, and increased again to 577 after biodegradation.