RESUMO
Concentrating properties of a new adsorbent, active aluminum oxide, towards poliomyelitis virus type III and simian rotavirus are studied using virus contamination of sewage and drinking water. Optimal concentrations of the adsorbent for effective adsorption of both rota- and polioviruses are established (1.5 and 1 g/liter, respectively) at pH typical of sewage and drinking water (7.0-8.5), as well as the optimal time of virus contact with the adsorbent (30 min). Elution conditions are determined: 3% elution agent beef extract and pH 8.5-9.5 are optimal for both viruses. Active aluminum oxide is recommended as an adsorbent for elimination of enteroviruses from water objects.
Assuntos
Óxido de Alumínio/química , Enterovirus/isolamento & purificação , Intestinos/virologia , Microbiologia da Água , Abastecimento de Água , Adsorção , Concentração de Íons de Hidrogênio , Esgotos/microbiologiaRESUMO
Experimental and clinical studies showed a number of virus infections to be accompanied by lipidemic disorders. Experimentally, dyslipidemias were found in tick-borne encephalitis (TBE) in mice, rotavirus infection in rabbits, and amyotrophic leukospongiosis in guinea pigs. The possibility of correcting the virus-induced lipidemic disorders with an antiviral drug, lincomycin, was demonstrated in TBE in mice. Dynamic study of the lipidemic status of patients with virus hepatitis A revealed marked dyslipidemia of the atherogenic type which was stable and persisted up to the time of clinical recovery. The data obtained supplement the current concepts concerning the pathogenesis of virus infections.
Assuntos
Encefalite Transmitida por Carrapatos/complicações , Hepatite A/complicações , Hiperlipidemias/etiologia , Doenças Priônicas/complicações , Infecções por Rotavirus/complicações , Adulto , Animais , Terapia Combinada , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Encefalite Transmitida por Carrapatos/sangue , Encefalite Transmitida por Carrapatos/tratamento farmacológico , Feminino , Cobaias , Hepatite A/sangue , Hepatite A/terapia , Humanos , Hiperlipidemias/sangue , Hiperlipidemias/epidemiologia , Lincomicina/uso terapêutico , Lipídeos/sangue , Lipoproteínas/sangue , Masculino , Camundongos , Doenças Priônicas/sangue , Coelhos , Fatores de Risco , Infecções por Rotavirus/sangue , Fatores de TempoRESUMO
The large immune stratum and intense collective immunity to virus hepatitis A among the urban population of Byelorussia are characteristic of hyperendemic territory. The geometric mean of the antibody titer has been noted to increase with age, which is probably due to repeated infections of persons who have already had the disease. The use of this value for the characterization of collective immunity and epidemiological situation has been proposed.
Assuntos
Hepatite A/imunologia , Adolescente , Adulto , Criança , Pré-Escolar , Hepatite A/epidemiologia , Anticorpos Anti-Hepatite A , Anticorpos Anti-Hepatite/análise , Hepatovirus/imunologia , Humanos , Técnicas Imunoenzimáticas , Lactente , Recém-Nascido , Pessoa de Meia-Idade , República de Belarus/epidemiologiaRESUMO
Human rotavirus strain 649 was isolated from a child with clinical picture of acute intestinal infection in the West European part of the U.S.S.R. This strain was adapted to MA-104 (rhesus monkey kidney) cells. By electrophoresis and haemagglutination it differed from the human rotavirus strain Wa and from the simian strain Sa-11. The productive reproduction of human rotavirus strain 649 may allow its use for the preparation of antigens for diagnostic, immunoprophylaxis, and treatment.
Assuntos
Gastroenterite/microbiologia , Infecções por Rotavirus/microbiologia , Rotavirus/isolamento & purificação , Animais , Anticorpos Antivirais/imunologia , Antígenos Virais/imunologia , Células Cultivadas , Reações Cruzadas , Fezes/microbiologia , Humanos , Rim , Macaca mulatta , Rotavirus/classificação , Rotavirus/imunologia , Cultura de VírusRESUMO
The spread of hepatitis A in children's institution occurred through everyday contacts, the boundaries of the focus of infection embraced the whole institution, and the active detection of the prevailing subclinical forms of the disease proved to be possible by means of virological and biomedical tests. The enhancement of the effectiveness of clinical and epidemiological surveillance by its orientation to the control of the spread of this infection through water and everyday contacts, the rational organization of sanitary, microbiological and serological studies, the development of criteria of epidemiological safety of children's institutions is proposed.
Assuntos
Creches/estatística & dados numéricos , Surtos de Doenças , Hepatite A/epidemiologia , Vigilância da População , Estações do Ano , Antígenos Virais/análise , Pré-Escolar , Fezes/análise , Hepatite A/imunologia , Hepatite A/transmissão , Anticorpos Anti-Hepatite/sangue , Hepatovirus/imunologia , Humanos , Lactente , República de Belarus/epidemiologia , Estudos Soroepidemiológicos , População Urbana/estatística & dados numéricosRESUMO
The possibility of using monkey red blood cells conserved by freezing at --196 degrees C in serological tests was studied. Hemagglutination and hemagglutination-inhibition tests with measles antigen and measles gamma globulin revealed no differences in the sensitivity of native and conserved red blood cells. The method of washing red blood cells from cryoprotective solution modified by the authors is described.
Assuntos
Preservação de Sangue/métodos , Eritrócitos/imunologia , Animais , Haplorrinos , Testes de Inibição da Hemaglutinação , Testes de Hemaglutinação , MacacaRESUMO
The pathogenic, infectious, and immunogenic properties of vaccinia virus were studied in various species of small laboratory animals (mice of different ages, Syrian hamsters, guinea pigs, white and cotton rats, rabbits). Cotton rats were found to be most susceptible to the infection. Vaccinia virus in this species causes an acute, frequently lethal infection. The inoculated virus intensively accumulated in parenchymatous organs, and this was accompanied by a marked rise of hemagglutinating and virus-neutralizing antibody levels.