Sex-dependent differences in macaque brain mitochondria.

Mitochondrial bioenergetics in females and males is different. However, whether mitochondria from male and female brains display differences in enzymes of oxidative phosphorylation remains unknown. Therefore, we characterized mitochondrial complexes from the brains of male and female macaques (Macaca mulatta). Cerebral tissue from male macaques exhibits elevated content and activity of mitochondrial complex I (NADH:ubiquinone oxidoreductase) and higher activity of complex II (succinate dehydrogenase) compared to females. No significant differences between sexes were found in the content of α-ketoglutarate dehydrogenase or in the activities of cytochrome c oxidase and F1Fo ATPase. Our results underscore the need for further investigations to elucidate sex-related mitochondrial differences in humans.

Caracterización de los desenlaces quirúrgicos a corto plazo en gastrectomía por laparoscopia en un centro de alto volumen: 10 años de experiencia / Short-term surgical outcomes of laparoscopic gastrectomy in a high-volume center: 10-year experience

Introducción. El cáncer gástrico en Colombia es la segunda neoplasia más común en hombres y la cuarta en mujeres. En los últimos años se han descrito ampliamente los beneficios del abordaje laparoscópico en el cáncer gástrico frente a sangrado, recuperación postoperatoria y complicaciones, sin afectar los resultados oncológicos. Métodos. Estudio observacional retrospectivo de pacientes llevados a gastrectomía laparoscópica en la Clínica Universitaria Colombia durante un período de diez años, entre 2013 y 2023. Se describieron los resultados perioperatorios en cuanto a estancia hospitalaria, sangrado operatorio, duración del procedimiento, complicaciones, causas de reintervención y mortalidad en los primeros 30 días. Resultados. Se incluyeron 418 pacientes, 58,9 % hombres, con una edad promedio de 60,8 años. Se documentó un tiempo quirúrgico promedio de 228,7 minutos, con un sangrado de 150 ml. La media de ganglios linfáticos resecados fue de 26,1 ± 11,4. La estancia hospitalaria en promedio fue de 4 ± 4 días, y se registraron complicaciones en 104 sujetos, con una tasa promedio de 24 %, de las cuales 29 (27,4 %) obtuvieron una clasificación Clavien-Dindo IIIB. Conclusiones. La gastrectomía por laparoscopia en un centro de alto volumen y con cirujanos experimentados en Colombia, tiene resultados perioperatorios similares a lo reportado en la literatura mundial. Aún se requiere de estudios de mayor fuerza de asociación para establecer recomendaciones sobre el uso rutinario de este abordaje en patología maligna avanzada.

Introduction. Gastric cancer in Colombia is the second most common neoplasm in men and the fourth in women. In recent years, the benefits of the laparoscopic approach in gastric cancer against bleeding, postoperative recovery com and complications have been widely described, without affecting oncological results. Methods. Retrospective observational study of patients undergoing laparoscopic gastrectomy at the Clínica Universitaria Colombia over a period of ten years, between 2013 and 2023. Perioperative results were described in terms of hospital stay, operative bleeding, duration of the procedure, complications, causes of reintervention, and mortality in the first 30 days. Results. 418 patients were included, 58.9% men, with an average age of 60.88 years. An average surgical time of 228.7 minutes was documented, with a blood loss of 150 ml. The mean number of lymph nodes resected was 26.1 ± 11.4. The average hospital stay was 4 ± 4 days, and complications were recorded in 104 subjects, with an average rate of 24%, of which 29 (27.4%) obtained a Clavien-Dindo IIIB classification. Conclusions. Laparoscopic gastrectomy in a high-volume center and with experienced surgeons in Colombia has perioperative results similar to those reported in the world literature. Studies with greater strength of association are still required to establish recommendations on the routine use of this approach in advanced malignant pathology.

Del COVID leve al síndrome persistente: evaluación integral de la salud oral / From mild COVID to long COVID: comprehensive oral health assessment

Introducción: El COVID-19 ha causado una amplia sintomatología, incluyendo la presente en la cavidad oral. Cada día cobra más importancia un nuevo síndrome relacionado: el COVID persistente. El objetivo de este trabajo es analizar el efecto de la infección por SARS-CoV-2 a nivel oral en sujetos diagnosticados de COVID persistente, en comparación con la infección aguda. Métodos: Se llevó a cabo un estudio de casos y controles con 102 sujetos reclutados entre 2021 y 2022, de los que se obtuvieron 34 variables de salud oral y posibles factores de riesgo. Resultados: El análisis estadístico reveló que los sujetos COVID persistente presentaban significativamente mayor prevalencia de: adenopatías, dolor de ATM, irritación faríngea, xerostomía, obturaciones, ausencias y coronas dentales, mayor valor en índices CAOM y CAOD y mayor número de síntomas odontológicos en total. Además, el estrés apareció como factor de riesgo; aquellos pacientes con COVID persistente que presentaron mayor nivel de estrés (7,73 ± 2,02) también eran los que sufrían, en mayor medida, xerostomía o bruxismo, responsable del dolor de ATM, también más prevalente en este grupo. Conclusiones: El COVID persistente provoca manifestaciones orales relacionadas, algunas de ellas, con el hecho de que la cavidad oral sea vía de entrada del virus, como la irritación mucosa; otras, relacionadas con su posible naturaleza autoinmune, como la xerostomía y, de la misma manera, otras relacionadas con el estrés, reflejado en la presencia de bruxismo. Resulta imprescindible desarrollar protocolos que mejoren tanto el diagnóstico precoz como el manejo de estos pacientes en nuestras clínicas. (AU)

Introduction: COVID-19 has caused a wide range of symptomatology, including that present in the oral cavity. A new related syndrome is gaining importance: Long COVID. The aim of this work is to analyse the effect of SARS-CoV-2 infection at the oral level in subjects diagnosed with Long COVID, compared to acute infection. Methods: A case-control study was conducted with 102 subjects recruited between 2021 and 2022, from whom 34 oral health variables and possible risk factors were obtained. Results: Statistical analysis revealed that Long COVID subjects had significantly higher prevalence of: adenopathies, TMJ pain, pharyngeal irritation, xerostomia, fillings, dental absences and dental crowns, higher CAOM and CAOD index values and higher total dental symptoms. In addition, stress appeared as a risk factor; those patients with Long COVID who presented a higher level of stress (7.73 ± 2.02) were also those who suffered, to a greater extent, from xerostomia or bruxism, responsible for TMJ pain, also more prevalent in this group. Conclusions: Long COVID causes oral manifestations related, some of them, to the fact that the oral cavity is a route of entry of the virus, such as mucosal irritation; others, related to its possible autoimmune nature, such as xerostomia and, in the same way, others related to stress, reflected in the presence of bruxism. It is essential to develop protocols that improve both the early diagnosis and management of these patients in our clinics. (AU)

Protocolo de recuperación mejorada después de cirugía (ERAS) atenúa el estrés y acelera la recuperación en pacientes después de resección radical por cáncer colorrectal: Experiencia en la Clínica Universitaria Colombia / Enhanced Recovery After Surgery (ERAS) protocol attenuates stress and accelerates recovery in patients after radical resection for colorectal cancer: Experience at Clínica Universitaria Colombia

Introducción. El conjunto de estrategias de recuperación mejorada después de la cirugía (ERAS, por sus siglas en inglés) constituye un enfoque de atención multimodal y multidisciplinario, cuyo propósito es reducir el estrés perioperatorio de la cirugía, disminuir la morbilidad y acortar la estancia hospitalaria. Este estudio tuvo como objetivo describir los resultados clínicos de pacientes sometidos a cirugía por cáncer colorrectal, identificando las complicaciones principales y los factores perioperatorios relacionados con el alta temprana. Métodos. Se analizaron los pacientes consecutivos sometidos a cirugía colorrectal entre los años 2020 y 2023, todos los cuales siguieron el protocolo ERAS institucional. Se evaluaron las características clínicas, los factores perioperatorios, los desenlaces postoperatorios y la tasa global de adherencia al protocolo. Resultados. Un total de 456 pacientes fueron sometidos a cirugía colorrectal, 51% de sexo masculino, con edad media de 60 años. La mayoría de las intervenciones se realizaron por laparoscopia (78 %), con una tasa de conversión del 14,5 %. Las complicaciones postoperatorias incluyeron fuga anastomótica (4,6 %), sangrado, infección intraabdominal y obstrucción intestinal. La estancia hospitalaria promedio fue de 4 días y la mortalidad del 2,8 %. La tasa global de adherencia al protocolo ERAS fue del 84,7 %. Conclusiones. El enfoque combinado de cirugía laparoscópica y protocolo ERAS es factible, seguro y se asocia con una estancia hospitalaria más corta. La implementación y adherencia al protocolo ERAS no solo mejora los resultados postoperatorios, sino que también resalta la importancia de acceder a datos sólidos, permitiendo mejorar la atención perioperatoria local.

Introduction. The Enhanced Recovery After Surgery (ERAS) protocol is a multimodal, multidisciplinary approach to care, the purpose of which is to reduce the perioperative stress of surgery, decrease morbidity, and shorten hospital stay. This study aimed to describe the clinical outcomes of patients undergoing surgery for colorectal cancer, identifying the main complications and perioperative factors related to early discharge. Methods. Consecutive patients undergoing colorectal surgery between 2020 and 2023 were analyzed, who followed the institutional ERAS protocol. Clinical characteristics, perioperative factors, postoperative outcomes, and overall protocol adherence rate were evaluated. Results. A total of 456 patients underwent colorectal surgery, 51% male, with a mean age of 60 years. Most interventions were performed laparoscopically (78%), with a conversion rate of 14.5%. Postoperative complications included anastomotic leak (4.6%), followed by bleeding, intra-abdominal infection, and intestinal obstruction. The average hospital stay was 4 days and mortality was 2.8%. The overall adherence rate to the ERAS protocol was 84.7%. Conclusions. The combined approach of laparoscopic surgery and ERAS protocol is feasible, safe, and associated with a shorter hospital stay. Implementation and adherence to the ERAS protocol not only improves postoperative outcomes, but also highlights the importance of accessing solid data, allowing for improved local perioperative care.

Inhibitory Activity of 4-Benzylidene Oxazolones Derivatives of Cinnamic Acid on Human Acetylcholinesterase and Cognitive Improvements in a Mouse Model.

We synthesized seven (Z)-benzylidene-2-(E)-styryloxazol-5(4H)-ones derivatives of cinnamic acid and evaluated the ability of these compounds to inhibit human acetylcholinesterase (hAChE). The most potent compound was evaluated for cognitive improvement in short-term memory. The seven compounds reversibly inhibited the hAChE between 51 and 75% at 300 µM, showed an affinity (Ki) from 2 to 198 µM, and an IC50 from 9 to 246 µM. Molecular docking studies revealed that all binding moieties are involved in the non-covalent interactions with hAChE for all compounds. In addition, in silico pharmacokinetic analysis was carried out to predict the compounds' blood-brain barrier (BBB) permeability. The most potent inhibitor of hAChE significantly improved cognitive impairment in a modified Y-maze test (5 µmol/kg) and an Object Recognition Test (10 µmol/kg). Our results can help the rational design of hAChE inhibitors to work as potential candidates for treating cognitive disorders.

Evaluation of the Stability of a 1,8-Cineole Nanoemulsion and Its Fumigant Toxicity Effect against the Pests Tetranychus urticae, Rhopalosiphum maidis and Bemisia tabaci.

Pest control is a main concern in agriculture. Indiscriminate application of synthetic pesticides has caused negative impacts leading to the rapid development of resistance in arthropod pests. Plant secondary metabolites have been proposed as a safer alternative to conventional pesticides. Monoterpenoids have reported bioactivities against important pests; however, due to their high volatility, low water solubility and chemical instability, the application of these compounds has been limited. Nanosystems represent a potential vehicle for the broad application of monoterpenoids. In this study, an 1,8-cineole nanoemulsion was prepared by the low energy method of phase inversion, characterization of droplet size distribution and polydispersity index (PDI) was carried out by dynamic light scattering and stability was evaluated by centrifugation and Turbiscan analysis. Fumigant bioactivity was evaluated against Tetranychus urticae, Rhopalosiphum maidis and Bemisia tabaci. A nanoemulsion with oil:surfactant:water ratio of 0.5:1:8.5 had a droplet size of 14.7 nm and PDI of 0.178. Formulation was stable after centrifugation and the Turbiscan analysis showed no particle migration and a delta backscattering of ±1%. Nanoemulsion exhibited around 50% more bioactivity as a fumigant on arthropods when compared to free monoterpenoid. These results suggest that nanoformulations can provide volatile compounds of protection against volatilization, improving their bioactivity.

In Silico Characterization of the Physicochemical and Biological Properties of the Pink (Pleurotus djamor var. salmoneostramineus) Oyster Mushroom Chromoprotein.

Cap color is an important commercial trait for oyster mushrooms. Various pigment constituents determine a diverse color. However, the pigments of oyster mushrooms are still ambiguous. The pink oyster mushroom (Pleurotus salmoneostramineus or Pleurotus djamor) chromoprotein is one of the few proteins belonging to this fungus that has a record of its sequence of amino acid residues. However, even though there are studies about this chromoprotein isolation, purification, and crystallization, the current information focused on its 3-dimensional model and the cofactor and prosthetic group (3H-indol-3-one) binding sites is unreliable and fragmented. Therefore, in this study, using free online servers such as Prot pi, GalaxyWEB, MIB, and CB-Dock2, a structural analysis and the prediction of its physicochemical and biological properties were conducted, to understand the possible function of this chromoprotein. The obtained results showed that this molecule is a protein with a molecular weight of 23 712.5 Da, an isoelectric point of 7.505, with oligomerization capacity in a dimer and glycation in the Ser6 residue. In addition, the participation of the residues Leu5, Leu8, Lys211, Ala214, and Gln215 in the binding of the prosthetic group to the protein was highlighted; as well as Ser6 and Pro7 are important residues for the interaction of the Mg2+ ion and eumelanin. Likewise, morphological changes based on different culture conditions (light/dark) showed that this protein is constitutive expressed and independent of blue light. The findings in this study demonstrate that pink chromoprotein is a melanosomal protein, and it possibly has a critical role in melanogenesis and the melanin polymerization. However, more experimental studies are needed to predict a possible mechanism of action and type of enzymatic activity.

The Hibiscus Model: A Feasible Cadaveric Model Using Continuous Arterial Circulation for Intracranial Bypass Training and Its Validation.

OBJECTIVE: The frequency of intracranial bypass procedures has declined. Thus it is difficult for neurosurgeons to develop the necessary skills for this complex procedure. We present a perfusion-based cadaveric model to provide a realistic training experience with high anatomic and physiological fidelity, as well as instantaneous assessment of bypass patency. Validation was assessed by evaluating the educational impact and skill improvement of the participants. METHODS: Fourteen participants attended a hands-on revascularization course with 7 cadaveric models connected to a continuous arterial circulation system pumping a red-colored solution through the entire cranial vasculature, mimicking blood circulation. The ability to perform a vascular anastomosis was evaluated initially. Further, a questionnaire on prior experience was provided. At the end of the 36-hour course, the ability to perform an intracranial bypass was reexamined and the participants completed a self-assessment questionnaire. RESULTS: Initially, only 3 attendees were able to perform an end-to-end anastomosis within the time limit, and only 2 of these anastomoses showed adequate patency. After having accomplished the course, all participants were able to complete a patent end-to-end anastomosis within the time limit, thus demonstrating a significant improvement. Further, both overall educational gain and surgical skills were regarded as remarkable (n = 11 and n = 9). CONCLUSIONS: Simulation-based education is considered an important aspect of medical and surgical development. The presented model is a feasible and accessible alternative to the prior models used for cerebral bypass training. This training may serve as a helpful and widely available tool to improve neurosurgeons' development irrespective of financial resources.

eIF4A/PDCD4 Pathway, a Factor for Doxorubicin Chemoresistance in a Triple-Negative Breast Cancer Cell Model.

Cells employ several adaptive mechanisms under conditions of accelerated cell division, such as the unfolded protein response (UPR). The UPR is composed of a tripartite signaling system that involves ATF6, PERK, and IRE1, which maintain protein homeostasis (proteostasis). However, deregulation of protein translation initiation could be associated with breast cancer (BC) chemoresistance. Specifically, eukaryotic initiation factor-4A (eIF4A) is involved in the unfolding of the secondary structures of several mRNAs at the 5' untranslated region (5'-UTR), as well as in the regulation of targets involved in chemoresistance. Importantly, the tumor suppressor gene PDCD4 could modulate this process. This regulation might be disrupted in chemoresistant triple negative-BC (TNBC) cells. Therefore, we characterized the effect of doxorubicin (Dox), a commonly used anthracycline medication, on human breast carcinoma MDA-MB-231 cells. Here, we generated and characterized models of Dox chemoresistance, and chemoresistant cells exhibited lower Dox internalization levels followed by alteration of the IRE1 and PERK arms of the UPR and triggering of the antioxidant Nrf2 axis. Critically, chemoresistant cells exhibited PDCD4 downregulation, which coincided with a reduction in eIF4A interaction, suggesting a sophisticated regulation of protein translation. Likewise, Dox-induced chemoresistance was associated with alterations in cellular migration and invasion, which are key cancer hallmarks, coupled with changes in focal adhesion kinase (FAK) activation and secretion of matrix metalloproteinase-9 (MMP-9). Moreover, eIF4A knockdown via siRNA and its overexpression in chemoresistant cells suggested that eIF4A regulates FAK. Pro-atherogenic low-density lipoproteins (LDL) promoted cellular invasion in parental and chemoresistant cells in an MMP-9-dependent manner. Moreover, Dox only inhibited parental cell invasion. Significantly, chemoresistance was modulated by cryptotanshinone (Cry), a natural terpene purified from the roots of Salvia brandegeei. Cry and Dox co-exposure induced chemosensitization, connected with the Cry effect on eIF4A interaction. We further demonstrated the Cry binding capability on eIF4A and in silico assays suggest Cry inhibition on the RNA-processing domain. Therefore, strategic disruption of protein translation initiation is a druggable pathway by natural compounds during chemoresistance in TNBC. However, plasmatic LDL levels should be closely monitored throughout treatment.

Cordyceps militaris Inhibited Angiotensin-Converting Enzyme through Molecular Interaction between Cordycepin and ACE C-Domain.

One of the most important therapeutic modalities for the management of hypertension is the inhibition of the angiotensin-converting enzyme (ACE). Cordyceps militaris has received substantial attention because to its therapeutic potential and biological value. To gather information about the antihypertensive properties of C. militaris, the ACE inhibitory activity was evaluated. An ethanolic extract of the fruiting body of C. militaris was obtained, and the extract was separated by UHPLC method with a fluorescence detector for the quantification of cordycepin and adenosine. The ethanolic extract had a considerably higher cordycepin level. Additionally, an in vitro kinetic analysis was carried out to find out how much C. militaris extract inhibited ACE. This extract exhibited non-competitive inhibition on ACE. The Ki value of the C. militaris extract against ACE was found to be 8.7 µg/mL. To the best of our knowledge, this is the first report of the analysis of a protein cavity together with molecular docking carried out to comprehend the intermolecular interactions between cordycepin and the ACE C-domain, which impact the spatial conformation of the enzyme and reduce its capacity to break down the substrate. According to a molecular docking, hydrogen bonding interactions between the chemicals and the ACE S2' subsite are primarily responsible for cordycepin inhibition at the ACE C domain. All these findings suggest that C. militaris extract are a kind of natural ACE inhibitor, and cordycepin has the potential as an ACE inhibitor.

A retrospective study on the effect of the COVID-19 pandemic on dental treatments in adults.

BACKGROUND: The aim was to analyze the prevalence of dental treatments that were not performed in a dental care university referral center in the capital of Spain during the first wave of the COVID-19 pandemic. METHODS: This was a retrospective observational study based on the registry of medical records. Sex, age, nationality, and the type of treatment that was not performed in the service of the Integrated Adult Dental Clinic subject of the Dentistry degree at the Rey Juan Carlos University of Madrid were analyzed. RESULTS: A total of 392 medical records were analyzed. The prevalence of the treatments that were not performed was 58.67% (95% CI 53.74-63.44) of conservative treatments, 47.45% (95% CI 42.55-52.39) of periodontal treatments, 27.30% (95% CI 23.12-31.91) and 13.52% (95% CI 10.49-17.26) of clinical activities. The patients most affected by the absence of dental treatment ranged in age from 35 to 74 years. Age, sex, and nationality were not influential in not performing dental treatments. CONCLUSIONS: The COVID-19 pandemic could have negatively influenced treatments, such as conservative and periodontal treatments, that increasing the risk of tooth loss in adults.

The IWH-BEAT Questionnaire Validation.

Positive Psychology has been devoted to enhancing well-being within organizations during the first two decades of the current millennium. Unfortunately, little data is available on current assessment related to positive psychology practices in the workplace. Therefore, to assess organizational well-being in a valid and reliable way, a new scale has been created and validated by the Institute for Wellbeing and Happiness at Tecmilenio University in Mexico: the BEAT Questionnaire whose main contribution to previous models is the element of meaningful work. EFA and CFA were carried out to determine and confirm the scale's structure; internal consistency tests were performed too; additionally, convergence with measures of engagement, labor resources, and worker relations were also confirmed, and discriminant validity was tested by comparing associations with job search intentions and negative relationships in the workplace, yielding an instrument with four clearly defined latent dimensions, composed by 24 highly consistent items, convergent with three other valid and reliable scales. All procedures complied with statistical requirements, delivering a valid and reliable instrument for measuring well-being in the workplace.

La enfermedad renal crónica. Revisión de la literatura y experiencia local en una ciudad de Ecuador

La enfermedad renal crónica (ERC) representa una problemática importante de salud pública a nivel mundial, pues tiene implicaciones clínicas y socioeconómicas significativas. Lo anterior ha motivado a incrementar los esfuerzos por parte de los sistemas sanitarios para mejorar su detección precoz, así como la coordinación entre las distintas especialidades médicas. La evidencia científica ha demostrado que, en la actualidad, la incidencia de la ERC incrementa en correlación con el proceso de envejecimiento, siendo este uno de los factores asociados más importantes a tener en cuenta, junto con las enfermedades crónicas no transmisibles. Por lo tanto, se hace relevante realizar una revisión de la literatura disponible para exponer la situación actual de la ERC a nivel mundial y poderla comparar con la experiencia local de la ciudad de Ambato, Ecuador, esto desde un enfoque nefroprotector.

Chronic kidney disease (CKD) represents a major public health problem. It has significant clinical and socioeconomic implications. This disease increases the efforts of the health system for its early detection and to improve coordination between the different medical specialties. Scientific evidence has shown that the incidence of CKD increases in the population in correlation to the aging process and chronic non-transmissible diseases. Therefore, it is relevant to review the literature to expose the current situation of CKD worldwide and compare it with the local experience from Ambato, Ecuador, with a nephroprotective approach.

Parámetros productivos y digestibilidad de pollos, utilizando cayeno (Hibiscus rosa-sinensis) y probiótico (Lactobacilius acidophilus) más pectina / Productive parameters and digestibility of chickens, using cayenne (Hibiscus rosa-sinensis) and probiotic (Lactobacilius acidophilus) plus pectin / Parâmetros produtivos e digestibilidade de frango, utilizando pimenta-caiena (Hibiscus rosa-sinensis) e probiótico (Lactobacilius acidophilus) mais pectina

resumen está disponible en el texto completo

Abstract The growth in broiler chicken production has been a determining factor regarding Colombia's economy and a determining factor in promoting the countryside's economic development. Using probiotics containing lactic acid-producing bacteria in broiler chickens' diet contributes to intestinal flora integrity and stability, thereby hampering pathogen proliferation which helps prevent diseases and improve productivity. This research was aimed at evaluating broiler chicken nutrient digestibility and production parameters by including cayenne pepper flour (Hibiscus rosa-sinensis, L.) with and without a probiotic (Lactobacillus acidophilus) plus pectin in chicken feed to replace concentrated food. This project was carried out in Villavicencio in Colombia's Meta department; 90 broilers were fed on broiler starter concentrate for 15 days. T1 treatment consisted of concentrate commercial (CC) milled crumble, T2 CC plus 6% cayenne flour (CF) and T3 CC plus 12% CF; these treatments were compared with a probiotic (WP) and without it (WoP) plus pectin (PP). Cayenne pepper green stems and leaves were harvested 60 days after the last pruning for making CF; they were dehydrated for 72 hours at 60oC and then ground. The chickens' average weight was 675.1±50g; a randomised complete block design (RCBD) was used, along with Tukey's multiple comparison test for statistical analysis of the resulting data. Excreta were collected over a five-day period for estimating digestibility coefficients (DC). Broiler diets and excreta were analysed regarding dry matter (DM), protein, fibre, fat, non-nitrogenous extract (NNE) and neutral detergent fibre (NDF); % total digestible nutrients (TDN) and protein biological value (BV) were estimated. Production variables such as food consumption rate, weight gain, feed conversion and carcass yield were evaluated. T2 had the highest DM WoP DC (0.91) and DM WP DC (0.93) compared to those for T1 (0.71 DM WoP DC and DM WP DC 0.72) (p<0.05); the same happened for T2 protein (0.88 DM WoP DC and 0.92 DM WP DC) and fibre (0.82 DM WoP DC and 0.84 DM WP DC), such DC being greater (p<0.05) than those for T3 (protein 0.72 DM WoP DC and 0.81 DM WP DC and fibre 0.56 DM WoP DC and 0.57 DM WP DC). T2 had the highest TDN value: 92 (83%) compared to T3: 77 (56%). All treatments had DM similar consumption rates. Daily weight gain was higher (p<0.05) for T1 WoP (93.74g) and WP (89.78g) compared to T3 (84.08g T1 WoP and 81.95g WP); no differences were observed regarding T2. T2 WoP (2.0) and T3 WP (1.95) had the lowest food to weight conversion rates; the probiotic had an effect on this because T2 WP (1.74) was similar to T1 WoP (1.81) and WP (1.86). T1 WP (66.12%) and WoP (65.49%) and T2 WP (64.92%) had promising carcass yields compared to that for the other treatments. There were no differences between T2 and T1 regarding most variables evaluated here, meaning that commercial concentrate can be confidently replaced by 6% cayenne flour in broiler diets during their fattening phase.

Resumo O crescimento da produção avícola tem sido um fator determinante na economia, sendo um dos motores para promover o desenvolvimento econômico do campo colombiano. O uso de probióticos que contêm bactérias produtoras de ácido lático na ração de aves contribui para a integridade e estabilidade da flora intestinal, dificultando a proliferação de patógenos, o que ajuda a prevenir doenças e melhorar o desempenho produtivo. O objetivo desta pesquisa foi avaliar a digestibilidade dos nutrientes e parâmetros produtivos em frangos de corte incluindo a farinha de pimenta de Caiena (Hibiscus rosa sinensis) sem e com probiótico (Lactobacilius acidophilus) mais pectina em substituição ao concentrado. Este projeto foi realizado em Villavicencio, Meta, foram utilizados 90 frangos, durante 15 dias, eles foram alimentados com concentrado inicial. Os tratamentos foram: T1 concentrado comercial moído (CC), T2 CC e farinha de pimenta-caiena 6% (HC) e T3 CC e 12% HC, comparando-se esses tratamentos sem (SP) e com probiótico mais pectina (CP). Para a produção do HC, os caules verdes e as folhas de caiena foram colhidos 60 dias após o último corte, foram desidratados por 72 horas a 60 graus centígrados e moídos. O peso médio dos frangos foi de 675,1 ± 50g, sendo distribuídos em delineamento inteiramente casualizado e aplicado o teste de comparações múltiplas de Tukey. Para estimar os coeficientes de digestibilidade (COD), as excretas foram coletadas por cinco dias. As dietas e excretas foram analisadas: matéria seca (MS), proteína, fibra, gordura, extrato não nitrogenado (ENN) e fibra em detergente neutro (FDN). Foram estimados: nutrientes digestíveis totais (NDT), valor biológico (VB) da proteína. Variáveis ​​produtivas como: consumo de ração, ganho de peso, conversão alimentar e rendimento de carcaça também foram avaliadas. O maior DM COD (P <0,05) foi para T2 SP e CP: 0,91 e 0,93 VS T1 0,71 e 0,72, o mesmo aconteceu com o COD de proteína e fibra de T2 sendo maior (P <0,05) em relação ao T3: 0,88 e 0,92 VS 0,72 e 0,81; 0,82 e 084 VS 0,56 e 0,57, respectivamente. O maior valor de NDT foi para T2: 92,83% VS T3: 77,56%. O consumo de matéria seca foi semelhante para todos os tratamentos. O ganho de peso diário foi maior (P <0,05) em T1 CP e SP 93,74 e 89,78 g em comparação com T3 84,08 e 81,95 g, não foram observadas diferenças com T2. As conversões de alimentação para peso mais baixas foram para T2 SP (2,0) e T3 CP (1,95). Observando o efeito do probiótico em T2 CP (1,74). O desempenho de carcaça foi semelhante para todos os tratamentos, embora valores numericamente superiores tenham sido observados em T1 CP e SP; T2 CP: 66,12, 65,49 e 64,92%), em relação aos demais tratamentos. Na maioria das variáveis ​​avaliadas, T2 não apresentou diferença com T1, o que significa que nas dietas para aves em fase de engorda, o concentrado comercial pode ser substituído por 6% de farinha de pimenta de caiena.

El reto del abordaje quirúrgico en la rehabilitación sobre un implante unitario en un caso de alto requerimiento estético del sector anterior. Descripción a propósito de un caso / The challenge of the surgical approach in the rehabilitation of a unitary implant in a case of high aesthetic requirements of the anterior sector. Case report

Objetivo: Describir de forma detallada el abordaje clínico basado en la evidencia científica actual de un implante postextracción con carga y provisionalización inmediata. Caso clínico: Se presenta el caso de una paciente mujer de 32 años de edad, que acude por presentar una posible fractura radicular del incisivo central superior izquierdo (ICSI), acompañada de la aparición de un absceso periodontal en la región del fondo de vestíbulo de dicho diente. Tras llevar a cabo la exploración clínica y radiológica, se establece que el pronóstico del ICSI es desfavorable para llevar a cabo un tratamiento conservador del mismo. Tras la valoración de las características clínicas del caso presente, el plan de tratamiento se inclinó por la realización de la exodoncia del ICSI con la colocación simultánea de un IOI postextracción y la carga inmediata con una prótesis provisional del mismo. Conclusiones: La rehabilitación sobre implantes en situaciones de pérdida dental en el sector anterior estético y en especial, en pacientes jóvenes, requiere un plan de tratamiento multidisciplinar en cuanto a la extracción del diente y colocación del IOI en la posición tridimensional correcta, existiendo diferentes aspectos a tener en cuenta para ello, especialmente el remanente óseo residual, la posición del margen gingival, así como la preservación y acondicionamiento de los tejidos duros y blandos periimplantarios mediante injertos y un manejo correcto de una prótesis provisional hasta conseguir un perfil de emergencia y contorno gingival ideal antes de la corona definitiva. (AU)

Objective: To describe in detail the clinical approach based on current scientific evidence for a post-extraction implant with immediate loading and provisionalisation. Clinical case: We present the case of a 32-year-old female patient who presented with a possible root fracture of the upper left central incisor (ULCI), accompanied by the appearance of a periodontal abscess in the region of the bottom of the vestibule of said tooth. After carrying out the clinical and radiological examination, it is established that the ULCI prognosis is unfavourable for carrying out conservative treatment of the tooth. After assessing the clinical characteristics of the present case, the chosen treatment plan was to extract the ULCI with the simultaneous placement of a post-extraction osseointegrated implant (OII) and immediate loading of a provisional prosthesis on the implant. Conclusions: Rehabilitation on implants in situations of tooth loss in the aesthetic anterior sector, especially in young patients, requires a multidisciplinary treatment plan in relation to tooth extraction and placement of the OII in the correct three-dimensional position. There are various aspects to be taken into account, particularly the residual remaining bone, the position of the gingival margin, as well as the preservation and conditioning of the peri-implant hard and soft tissues by means of grafts and proper handling of a provisional prosthesis until an ideal emergence profile and gingival contour is achieved before the final crown. (AU)

Patogénesis del dolor en la mucositis oral. Revisión de la literatura / Pathogenesis of pain in oral mucositis. Review of the literature

INTRODUCCIÓN: La mucositis oral es una lesión dolorosa que tiene lugar en la mucosa de la cavidad oral, normalmente su etiología se encuentra asociada a tratamientos farmacológicos en pacientes oncológicos. Se presenta como úlceras bien delimitadas cuya sintomatología dolorosa supone en ocasiones la suspensión del tratamiento oncológico o la alimentación por vía parenteral, siendo por tanto un efecto adverso importante, marcando el devenir en este tipo de terapias contra el cáncer. OBJETIVO: El objetivo del presente artículo es poner en relieve cómo se produce el dolor en esta patología que acontece en la mucosa de la cavidad oral. DISCUSIÓN: La mucositis oral se va a presentar tras una cascada de eventos biológicos que implican diferentes procesos moleculares tras el tratamiento con quimioterapia o radioterapia. El dolor en la mucositis oral puede poseer un componente inflamatorio y también un componente neuropático. En su fisiopatología, el dolor va a estar mediado por diferentes familias de receptores y factores. CONCLUSIÓN: La mucositis oral presenta un gran componente doloroso asociado, en el que cobran especial protagonismo en su aparición, las familias de los receptores y factores TRP, ET-1, TNF y ROS, entre otros. El conocimiento de la patogénesis del dolor en esta patología permitirá desarrollar terapéuticas contra el dolor en estudios futuros

INTRODUCTION: Oral mucositis is a painful lesion that occurs in the mucosa of the oral cavity. Its aetiology is usually associated with drug treatments in cancer patients. It presents as well-defined ulcers whose painful symptoms sometimes lead to the suspension of cancer treatment or parenteral nutrition. They therefore represent a significant adverse effect that marks the future in this type of cancer therapy. OBJECTIVE: The objective of this article is to highlight how pain occurs in this pathology that takes place in the mucosa of the oral cavity. DISCUSSION: Oral mucositis will occur following a cascade of biological events involving different molecular processes following treatment with chemotherapy or radiotherapy. Pain in oral mucositis may have an inflammatory component as well as a neuropathic component. In its pathophysiology, pain will be mediated by different families of receptors and factors. CONCLUSION: Oral mucositis has a large associated painful component, in which the families of TRP, ET-1, TNF and ROS receptors and factors, among others, play a major role in its appearance. Knowledge of the pathogenesis of the pain in this pathology will allow pain therapies to be developed in future studies

Third molar autotransplant planning with a tooth replica. A year of follow-up case report.

The advantages of dental autotransplantation and its high level of clinical success mean that it should be considered as a therapeutic option when replacing a lost tooth. In order to achieve optimum results, it is necessary to know the technique of dental autotransplantation, promoting its use whenever the clinical conditions to perform it are present. The objective of this article is to describe the technique in detail by means of a clinical case of a dental autotransplant whose donor tooth was a third unerupted molar. A 39-year-old male patient with no medical history of interest. On clinical examination, tooth 2.6 shows vertical fracture with indication of exodontia. A compatibility study is carried out using a CBCT and after this, a subsequent preparation of a 3D-printed replica of the donor tooth 2.8 is made. A step-by-step description is given of the autotransplantation technique from 2.8 to 2.6. After this, antibiotic coverage, semi-rigid splinting and root canal treatment are carried out in a short time. Results are shown at 12 months. The main factor for the success of this technique is the preservation of periodontal ligament cells. The unerupted teeth are the only ones that fully preserve the periodontal ligament, but they require greater surgical skills. Autotransplantation is a predictable treatment alternative to dental implants, being above all an option indicated to replace teeth with dental fissures or vertical root fractures or poor restorative and/or endodontic prognosis. The third molars are the most used teeth for transplantation, due to their indications for extraction in a high percentage preserving the entire periodontal ligament. The diagnosis by CBCT and the use of 3D- printed replicas of the tooth to be transplanted have meant a highly significant improvement in the prognosis and predictability of the technique. Key words:Dental autotransplant, tooth replica, third molar.

Inhibition of squalene synthase of rat liver by abietane diterpenes derivatives.

In the current study, chemical composition of cultivated Salvia canariensis L was determined. Carnosol was the main product isolated. We prepared more lipophilic derivatives from carnosol, and both isolated and semisynthetic abietane diterpenes were evaluated in vitro as inhibitors of squalene synthase. Among the compounds tested, carnosol was the most potent inhibitor (IC50 = 17.6 µM). These results highlight the great potential of this species for the production of new ingredients in nutritional supplements for the treatment of hyperlipidemia.

Tanshinones and their Derivatives: Heterocyclic Ring-Fused Diterpenes of Biological Interest.

The available scientific literature regarding tanshinones is very abundant, and after its review, it is noticeable that most of the articles focus on the properties of tanshinone I, cryptotanshinone, tanshinone IIA, sodium tanshinone IIA sulfonate and the dried root extract of Salvia miltiorrhiza (Tan- Shen). However, although these products have demonstrated important biological properties in both in vitro and in vivo models, their poor solubility and bioavailability have limited their clinical applications. For these reasons, many studies have focused on the search for new pharmaceutical formulations for tanshinones, as well as the synthesis of new derivatives that improve their biological properties. To provide new insights into the critical path ahead, we systemically reviewed the most recent advances (reported since 2015) on tanshinones in scientific databases (PubMed, Web of Science, Medline, Scopus, and Clinical Trials). With a broader perspective, we offer an update on the last five years of new research on these quinones, focusing on their synthesis, biological activity on noncommunicable diseases and drug delivery systems, to support future research on its clinical applications.

Medium cut-off dialyzers in a large population of hemodialysis patients in Colombia: COREXH registry.

Expanded hemodialysis (HDx) provides increased clearance of conventional and large middle molecules through innovative medium cutoff (MCO) membranes. However, there is a paucity of real-world data regarding the benefits and safety of HDx. This large observational study evaluated outcomes among patients in Colombia undergoing HDx at a extended dialysis clinical services provider. This was a prospective single cohort study of prevalent patients who were treated with HDx; baseline information was collected from the most recent data before patients were started on HDx. Patients were followed prospectively for 1 year for changes in serum albumin and other laboratory parameters compared with the baseline. Survival, hospitalization and safety were assessed from the start of HDx. A total of 1000 patients were invited to enroll; 992 patients met the inclusion criteria for data analysis and 638 patients completed the year of follow-up. Seventy-four (8%) patients died during 866 patient-years (PY) of follow-up; the mortality rate was 8.54 deaths/100 PY (95% confidence interval [CI], 6.8-10.7). There were 673 hospitalization events with a rate of 0.79 events/PY (95% CI, 0.73-0.85) with 6.91 hospital days/PY (95% CI, 6.74-7.09). The observed variability from baseline and maximum average change in mean serum albumin levels were -1.8% and -3.5%, respectively. No adverse events were related to the MCO membrane. HDx using an MCO membrane maintains stable serum albumin levels and is safe in terms of nonoccurrence of dialyzer related adverse events.