Transfer and degradation of polyacrylamide-based flocculants in hydrosystems: a review.

The aim of this review was to summarize information and scientific data from the literature dedicated to the fate of polyacrylamide (PAM)-based flocculants in hydrosystems. Flocculants, usually composed of PAMs, are widely used in several industrial fields, particularly in minerals extraction, to enhance solid/liquid separation in water containing suspended matter. These polymers can contain residual monomer of acrylamide (AMD), which is known to be a toxic compound. This review focuses on the mechanisms of transfer and degradation, which can affect both PAM and residual AMD, with a special attention given to the potential release of AMD during PAM degradation. Due to the ability of PAM to adsorb onto mineral particles, its transport in surface water, groundwater, and soils is rather limited and restricted to specific conditions. PAM can also be a subject of biodegradation, photodegradation, and mechanical degradation, but most of the studies report slow degradation rates without AMD release. On the contrary, the adsorption of AMD onto particles is very low, which could favor its transfer in surface waters and groundwater. However, AMD transfer is likely to be limited by quick microbial degradation.

Microbial aerobic and anaerobic degradation of acrylamide in sludge and water under environmental conditions--case study in a sand and gravel quarry.

Polyacrylamides (PAMs) are used in sand and gravel quarries as water purification flocculants for recycling process water in a recycling loop system where the flocculants remove fine particles in the form of sludge. The PAM-based flocculants, however, contain residual amounts of acrylamide (AMD) that did not react during the polymerization process. This acrylamide is released into the environment when the sludge is discharged into a settling basin. Here, we explore the microbial diversity and the potential for AMD biodegradation in water and sludge samples collected in a quarry site submitted to low AMD concentrations. The microbial diversity, analyzed by culture-dependent methods and the denaturing gradient gel electrophoresis approach, reveals the presence of Proteobacteria, Cyanobacteria, and Actinobacteria, among which some species are known to have an AMD biodegradation activity. Results also show that the two main parts of the water recycling loop-the washing process and the settling basin-display significantly different bacterial profiles. The exposure time with residual AMD could, thus, be one of the parameters that lead to a selection of specific bacterial species. AMD degradation experiments with 0.5 g L(-1) AMD showed a high potential for biodegradation in all parts of the washing process, except the make-up water. The AMD biodegradation potential in samples collected from the washing process and settling basin was also analyzed taking into account on-site conditions: low (12 °C) and high (25 °C) temperatures reflecting the winter and summer seasons, and AMD concentrations of 50 µg L(-1). Batch tests showed rapid (as little as 18 h) AMD biodegradation under aerobic and anaerobic conditions at both the winter and summer temperatures, although there was a greater lag time before activity started with the AMD biodegradation at 12 °C. This study, thus, demonstrates that bacteria present in sludge and water samples exert an in situ and rapid biodegradation of AMD at low concentration, whatever the season, and in both the aerobic and anaerobic parts of the water recycling system.