Binding properties of mono- and dimeric pyridine dicarboxamide ligands to human telomeric higher-order G-quadruplex structures.

Here, we report on the in vitro binding properties of the known pyridine dicarboxamide G-quadruplex ligand 360A and a new dimeric analogue (360A)2A to human telomeric DNA higher-order G-quadruplex (G4) structures. This study points to original binding features never reported for G4 ligands, and reveals a greater efficiency for the dimeric ligand to displace RPA (a ssDNA binding protein involved in telomere replication) from telomeric DNA.

[Plasma levels of HCS and beta SP1 in normal and pathological pregnancies]. / Dosage plasmatique de HCS et betaSP1 dans les grossesses normalies et pathologiques.

A study was carried out on 599 pregnant women from whom 2,991 levels were estimated of HCS and beta SP1 to determine their variations. The control group was of 400 normal pregnancies (1,770 couples of levels of HCS and beta SP1. The pathological population was in two groups: - non-complicated pathological pregnancies, from whom 510 coupled levels were estimated in 77 women, and - complicated pregnancies, from whom 711 coupled levels were estimated in 122 patients. This pathological group was compared with the control population statistically. The results conform to those that have already been published. This work shows two original features: - very high levels of beta SP1 and HCS ;are an index of the severity of the prognosis of fetuses and hydramnios, - a drop in the level of beta SP1 towards the end of pregnancies is a warning that fetal distress may occur in labour.