Estudio de citoquinas y proteínas de choque térmico en pacientes polifracturados / Study of cytokines and heat shock proteins in multiple fracture patients

Objetivo: Valorar la repercusión y comportamiento de los niveles séricos de factores proinflamatorios con respecto a la aparición de complicaciones médico–quirúrgicas en pacientes politraumatizados. Material y métodos: se incluyeron 18 pacientes politraumatizados, 10 hombres y 8 mujeres, con 2 o más fracturas óseas y un ISS>16, con edad media de 42 años y cuyo mecanismo lesional más frecuente fue el accidente de tráfico (44%). El valor medio del ISS fue de 26,83 y de 33,72 para el NISS. Se recogieron datos demográficos, lesiones ocasionadas, intervenciones quirúrgicas realizadas, datos de evolución, complicaciones y secuelas. Se analizó en sangre, leucocitos, fibrinógeno, proteína C reactiva, TNFα, interleucina 1β, interleucina 6, proteína de choque térmico HSP70i y anticuerpos antiHSP70i. Resultados: Los valores de TNFα, tienen una curva ascendente, con un aumento de la pendiente a partir de las 48 horas del traumatismo. La IL-1‚ mostró el pico máximo en la primera medición inmediatamente después del traumatismo, para disminuir de manera progresiva. La IL-6 presentó cifras por encima de 500 pg/ml. Los niveles séricos elevados de HSP70i máximos en el momento inicial para disminuir en las siguientes 48 horas. Conclusiones: Las curvas de reacción de factores proinflamatorios establecidas servirán de base para futuros estudios que los afiancen como biomarcadores de politraumatismo (AU)

Objective: To evaluate the repercussion and behavior of the serum levels of proinflammatory factors in relation to the appearance of clinical-surgical complications in polytraumatized patients. Material and methods: The study comprised 18 polytraumatized patients, 10 males and 8 females, with two or more bone fractures and an injury severity score (ISS) >16, and with a mean age of 42 years, in which traffic accidents were the main cause of injury (44%). The mean ISS was 26.83, with a new injury severity score (NISS) of 33.72. Demographic data were collected, together with information on the injuries produced, the surgical interventions, outcome, complications and sequelae. Blood tests were performed to record leukocyte count, fibrinogen, C-reactive protein, TNF·, interleukin 1‚, interleukin 6, heat shock protein HSP70i and antiHSP70i antibodies. Results: The TNF· values showed an ascending tendency, with an increase in slope starting 48 hours after trauma. IL-1 in turn showed a maximum value on occasion of the first measurement immediately after injury, followed by a gradual decrease. IL-6 showed values above 500 pg/ml. Peak serum HSP70i elevation were recorded at first determination, followed by a decrease over the following 48 hours. Conclusions: The established proinflammatory factor response curves will serve as a basis for future studies to consolidate them as biomarkers applicable to polytraumatized patients (AU)

Estudio prospectivo de los reactantes fase aguda tras una artroplastia total de rodilla / Prospective study of acute phase response factors after total knee artrhoplasty

Introducción: La artroplastia total de rodilla actualmentees el tratamiento de elección para las artrosis evolucionadasde dicha articulación. Al igual que otras lesiones mayores, lasintervenciones quirúrgicas o los traumatismos, originan respuestasmetabólicas, hormonales y hemodinámicas. Nos proponemosvalorar los reactantes de fase aguda tanto en prótesistotales de rodilla normales como en infectadas.Material y método: De forma consecutiva, a partir de marzodel 2003, se reclutaron 36 pacientes a los que se les implantóuna prótesis total de rodilla (grupo I). Además se estudiaron10 pacientes (grupo II) a los que se les habíaimplantado una prótesis de rodilla y presentaban criterios deinfección.Resultado: En el grupo I, los leucocitos y la PCR presentanun pico máximo a las 24 horas de la intervención, observándoseniveles preoperatorios en la extracción obtenida al mes;El fibrinógeno y la VSG muestran un ascenso hasta la extraccióntomada al mes de la cirugía, y continúan con niveles superioresa los basales incluso después de tres meses.Lo pacientes del grupo II mostraron niveles, estadísticamentesignificativos, más altos de fibrinógeno, VSG y PCR.Conclusiones: Los reactantes de fase aguda ofrecen unamoderada eficacia diagnóstica por lo que deben apoyar aldiagnóstico basado en los criterios clínicos de infección

Introduction: Nowadays total knee arthroplasty (TKA) isthe choice treatment in evolved knee artrosis. Operations ortraumas cause metabolism and hormone responses.Patients and Methods: Consecutively, from 2003, we includedprospectively in this research, 36 patients undergoingelective TKA (group I). Also we studied 10 patients (group II)that were diagnosed as infected TKA.Results: Acute phase response evolution in group I showsmaximum leukocytes and C-reactive protein (CRP) levels duringthe first 24 hours after the surgery, returning to presurgicallevels after one month. Erythrocyte sedimentation (ESR)rate and fibrinogen are increased even 3 months after the surgery.The patients of the goup II showed hihger levels, statisticallysignificant, of fibrinogen, CRP and ESR.Discussion: Acute phase response factors offer modest diagnosticefficacy, so we always have to use the clinical criterionto achive a god diagnosis

[Predictive value of certain clinical parameters in the myelodysplastic syndromes]. / Carácter predictivo de determinados parámetros clínicos en los síndromes mielodisplásicos.

Myelodysplasic Syndromes (MDS) are a group of hematological entities whose feature is their evolution, in a variable percentage, to acute non-lymphoid leukemia (ANLL) with a very poor prognosis despite the new therapeutical advances. Our purpose was to study the predictive value of certain clinical parameters at the time of the diagnosis, in order to identify the patients with the worst prognosis and, thus, the ones in which the adoption of more aggressive therapeutical measures than a simple support therapy would be justified. This is why we conducted a predictive analysis of 22 hematological variables (13 quantitative and 9 qualitative in 41 patients diagnosed of MDS). Based on the results of such study, we conclude that the average survival of the patients with MDS ranges between 11 and 33 months, 17% evolving to ANLL. The predictive parameters of poor prognosis are younger ages, leukocytary count, neutrophil and platelet count, as well as the degree of medullary blastosis. The following variables did not have any predictive value: sec, multinuclearity, macroblastic maturity, karyorrhexis, increase of immature forms, hypoglanularity in the white series and megakaryocytic count.

[Structural changes of the erythrocyte membrane in myelodysplastic syndromes]. / Alteraciones estructurales de la membrana eritrocitaria en los síndromes mielodisplásicos.

Myelodisplasic syndromes (MS) is a group of hematological alterations with well-known clinical, diagnosis, prognosis and therapeutic features. The etiology is still, however, unclear. Following the hypothesis of the lesion of the pluripotential cell, we have studied the composition of red cell membrane in 5 patients afflicted with MS (1 with refractory anemia; 3 refractory anemia plus excessive blast count and 1 chronic myelomonocytic leukemia). The red cells of the patients afflicted with MS has morphological alterations at examination in fresh. The most frequently observed being acanthocytosis and macrocytosis. Structurally, they showed an increase in the cholesterol/phospolipids (C/P) quotient, which was responsible for the increase of the cell area and macrocytosis. Meanwhile, the high level of sphingomyelin (SP) and its depletion of phosphatidil-ethanol-amine (PEA), justify the double population of red cells; one of which being acantocytes and the other has and aquired mutation of the genetic code, which confirms the chromosomic alterations widely described. We feel that our findings confirmed the hypothesis of the effects of an oncogen on the genoma of the mother cell, which can be causal of some acute leukemias and other genetically confirmed alterations.