The occurrence and human health risk assessment of microcystins in diverse food matrixes during production.

Globally, the presence of cyanotoxins in water supplies and food has been widely investigated for over a decade. Cyanotoxins are harmful metabolites produced by toxic cyanobacterial genera. These metabolites belong to diverse chemical classes, with a variety of physicochemical properties, chemical structures, and toxic activities. The present study seeks to investigate the occurrence of cyanotoxins in water supplies destined for food processing and assess the human health risk from exposure to cyanotoxins. To achieve this, a simple, sensitive, and reliable analytical method was developed for the determination of microcystins (MC-RR, MC-LR, MC-YR) in process water, raw maize meal, and cooked maize (porridge) at ppb (parts per billion) levels. These compounds were extracted using Solid Phase Extraction (SPE) with optimized parameters; thereafter, Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) was used for the rapid determination of the analytes selected for the present study. The method developed was applied to samples collected from the meal grinding station located in Mawoni village in South Africa; and was able to detect and quantify all the target cyanotoxins. MC-LR, MC-YR and MC-RR were detected at concentrations ranging from 10 to 11.2 µg/L, 9.1-9.4 µg/L, and 2.3-3.5 µg/L, in water samples, respectively. However, MC-YR was not detected in ground water sample. Moreover, MC-LR, MC-YR, and MC-RR concentrations in maize and porridge samples ranged between 9.2 and 11.2, 5.5-8.6, and 6.3-9.3 µg/kg dry weight, respectively. The hazard quotient index (HQi) levels found in the present study ranged between 2.2 - 8.4 and 0.11-8.9 for adults and children, respectively, representing potential risks to human health. Findings from LC-MS/MS reveal that cyanotoxins can be transferred from water to food during food processing using cyanotoxins contaminated water. Furthermore, the methods developed can be used by environmental and health agencies to strengthen the monitoring of cyanotoxins in water and food.

Using FlowCam and molecular techniques to assess the diversity of Cyanobacteria species in water used for food production.

Globally, the occurrence of cyanobacteria in water currently remains an important subject as they produce cyanotoxins that pose threat to human health. Studies on the contamination of maize meals during mill grinding processes using cyanobacteria-contaminated water have not been conducted. The present study aimed to assess the diversity of cyanobacteria in the samples (process water, uncooked maize meal, and cooked maize meal (porridge)). Polymerized Chain Reaction (PCR) and Advanced digital flow cytometry (FlowCAM) were used to detect and identify cyanobacterial species available in these samples. 16S Primers (forward and reverse) tailed with Universal Sequences were used for amplification and sequencing of full-length 16S rRNA genes from cyanobacteria found in all samples. Cyanobacterial species from order Nostocales, Pseudanabaenales, Oscillatoriales Chroococcales, Synechococcales, and unclassified cyanobacterial order, some of which have the potential to produce cyanotoxins were amplified and identified in process water, raw maize meal and porridge samples using PCR. Images of the genus Microcystis, Phormidium, and Leptolyngbya were captured in process water samples using FlowCAM. These findings show the presence of cyanobacteria species in process water used for maize meal and the absence in cooked maize meal. The presence of cyanobacteria in process water is likely another route of human exposure to cyanotoxins.

Retrospective analysis of Chlorophyll-a and its correlation with climate and hydrological variations in Mindu Dam, Morogoro, Tanzania.

The measurement of Chlorophyll-a in aquatic systems has usually correlated to harmful algae in water bodies. Harmful algal blooms (HABs) are as a result of massive proliferation of blue-green algae (Cyanobacteria). Harmful algal blooms (HABs) pose threats to both the environment as well as human health, and despite this well-known fact, their monitoring and management are still challenging. Climate change, extreme weather events, and hydrological changes are the main drivers and predicted to benefits HABs dynamics in most parts of the world. In Tanzania, studies of HABs proliferation and their possible correlation with variability in climate and hydrology still lag behind despite high demand for developing predicting tools and prevention of HABs proliferation. The present study reports on the retrospective analysis of HABs variation in Mindu Dam located in Morogoro, Tanzania using remote sensing techniques. In the present study comparison between in situ measurement and ocean color (OC2) Chlorophyll-a with the surface reflectance's (band and band combinations) of Landsat 7 and Landsat 8 Operational Land Imager (OLI), was performed. Another approach involved searching for patterns and trends, and teleconnection between Chlorophyll-a index (best band ration) and the climate and hydrological variations in the catchment. The findings demonstrated that minimum and maximum temperatures, solar radiation, Chlorophyll-a concentration registered significant increasing trends. Wind speed and directions, water levels for Mindu Dam showed a significant decreasing trend. On the other hand, rainfall showed no trend. The patterns suggest that there are link and causality between the HABs variations and meteorological parameters such as temperatures, solar radiations, and water levels. The study, therefore, contributes to the application of recent advances in remote sensing and retrospectively analysis of bloom dynamics and search for their link with climate and hydrological changes.

Algae colonisation of brick pavement at the University of Venda: A potential slippery hazard.

A brick pavement, tramped by humans, is exposed to atmospheric elements, thus allowing cyanobacteria and algae to colonise. In this article, we report on the factors that contribute to the slipperiness of a brick pavement at the University of Venda in the Limpopo province of the South Africa. Samples were collected from brick surfaces either colonised by green algae (treated) or not (control). The samples were acid-digested and analysed for metals by Inductively Coupled Plasma Mass Spectrometry (ICP MS) in parts per billion (ppb). The treated bricks, with green algae, had average high metal contents (ppb): Al 9456.02, Ti 731.23, V 46.44, Cr 78.85, Mn 862.93, Fe 16295.18, Co 23.57, Ni 59.36, Cu 66.31, Zn 160.57, As 7.92, Se 10.45, Mo 6.74, Cd 5.19, Sn 4.65, Sb 2.31 and Pb 19.51. In contrast, control bricks had a low average of metal content (ppb) as follows: Al 2.99, Ti 0.28, V 4.04, Cr 1.42, Mn 4.29, Fe 20.89, Co 0.36, Ni 2.74, Cu 5.64, Zn 4.21, As 0.56, Se <3.00, Mo 0.88, Cd 0.01, Sn 1.05, Sb 0.04 and Pb 0.04. Other factors that promote algae colonisation include high solar radiation, neutral pH, nutrients, low electrical conductivity and total dissolved solids. The algae colonisation of brick pavement results in an unaesthetic sighting and a slippery surface that is hazardous to humans.

Assessment of impact of land use change on the wetland in Makhitha village, Limpopo province, South Africa.

Wetlands are essential for the natural function of an ecosystem, by regulating floods and remaining as a source of water supply. However, land use changes are the main forces behind wetland deterioration worldwide, including South Africa. In this article, we report on the impact of land use changes at the Makhitha wetland, Limpopo province, South Africa. The data was collected using techniques such as ecological survey, socio-economic survey and interpretation of satellite images obtained between 1978 and 2004. The study findings revealed that factors such as poverty and population growth were the driving forces behind wetland mismanagement. The cultivation of crops, grazing of livestock and road construction were the main land use activities that were practised in the wetland, which contributed to wetland deterioration, a disaster that can be mitigated. The study then recommended strategies such as environmental education, fencing and land use planning to resolve the problem of land use changes, in order to contribute to sustainable wetland management.

The occurrence of cyanobacteria blooms in freshwater ecosystems and their link with hydro-meteorological and environmental variations in Tanzania.

Cyanobacteria (blue-green algae) are photosynthetic bacteria that under favorable environmental conditions produce secondary metabolites (cyanotoxins) which are harmful to the environment, including humans. The mass proliferation of harmful cyanobacteria is termed CyanoHABs. CyanoHABs can adapt to different climatic fluctuations, therefore, understanding their dynamics in freshwater systems is crucial. Variation in climatic and hydrological processes, changing land use and economic growth all influence the occurrence and distribution of CyanoHABs. There have been inadequate CyanoHAB studies at local scales, therefore their occurrence and dynamics cannot be generalized. This study reviews and synthesizes cases of CysnoHAB occurrence, magnitude, and timing and how these are linked with climatic and hydrological variations in the United Republic of Tanzania. In this study, a scoping review approach was adopted. Research articles, reports, and databases were consulted. The most common species of toxin-producing cyanobacteria were identified in different water bodies in Tanzania, as well as the record of mass fatality of birds (Lesser Flamingo) in Lake Manyara, which in almost all cases occurred during dry years. While previous studies on CyanoHAB dynamics and their links to climate, hydrological, and environmental changes have not been undertaken in Tanzania, there are studies in Lake Victoria and Tanganyika. Therefore, there should be an immediate response from water users, managers, researchers, and water authorities to address and actively engage in monitoring and managing the risks associated with CyanoHABs in Tanzania.

Synthesis and performance evaluation of Al/Fe oxide coated diatomaceous earth in groundwater defluoridation: Towards fluorosis mitigation.

The quest to reduce fluoride in groundwater to WHO acceptable limit of 1.5 mg/L to prevent diseases such as teeth mottling and skeletal fluorosis was the motivation for this study. Al/Fe oxide-modified diatomaceous earth was prepared and its defluoridation potential evaluated by batch method. The sorbent with pHpzc 6.0 ± 0.2 is very reactive. The maximum 82.3% fluoride removal attained in 50 min using a dosage of 0.3 g/100 mL in 10 mg/L fluoride was almost attained within 5 min contact time; 81.3% being the percent fluoride removal at 5 min contact time. The sorbent has a usage advantage of not requiring solution pH adjustment before it can exhibit its fluoride removal potential. A substantial amount of fluoride (93.1%) was removed from solution when a sorbent dosage of 0.6 g/100 mL was contacted with 10 mg/L fluoride solution for 50 min at a mixing rate of 200 rpm. The optimum adsorption capacity of the adsorbent was 7.633 mg/g using a solution containing initially 100 mg/L fluoride. The equilibrium pH of the suspensions ranged between 6.77 and 8.26 for 10 and 100 mg/L fluoride solutions respectively. Contacting the sorbent at a dosage of 0.6 g/100 mL with field water containing 5.53 mg/L at 200 rpm for 50 min reduced the fluoride content to 0.928 mg/L-a value below the upper limit of WHO guideline of 1.5 mg/L fluoride in drinking water. The sorption data fitted to both Langmuir and Freundlich isotherms but better with the former. The sorption data obeyed only the pseudo-second-order kinetic, which implies that fluoride was chemisorbed.

Assessment of heavy metals in municipal sewage sludge: a case study of Limpopo province, South Africa.

Heavy metals in high concentrations can cause health and environmental damage. Nanosilver is an emerging heavy metal which has a bright future of use in many applications. Here we report on the levels of silver and other heavy metals in municipal sewage sludge. Five towns in Limpopo province of South Africa were selected and the sludge from their wastewater treatment plants (WWTPs) was collected and analysed. The acid digested sewage sludge samples were analysed using Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) and Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) methods. The concentrations of silver found were low, but significant, in the range 0.22 to 21.93 mg/kg dry mass. The highest concentration of silver was found in Louis Trichardt town with a concentration of 21.93 ± 0.38 mg/kg dry mass while the lowest was Thohoyandou with a concentration of 6.13 ± 0.12 mg/kg dry mass. A control sludge sample from a pit latrine had trace levels of silver at 0.22 ± 0.01 mg/kg dry mass. The result showed that silver was indeed present in the wastewater sewage sludge and at present there is no DWAF guideline standard. The average Cd concentration was 3.10 mg/kg dry mass for Polokwane municipality. Polokwane and Louis Trichardt municipalities exhibited high levels of Pb, in excess DWAF guidelines, in sludge at 102.83 and 171.87 mg/kg respectfully. In all the WWTPs the zinc and copper concentrations were in excess of DWAF guidelines. The presence of heavy metals in the sewage sludge in excess of DWAF guidelines presents environmental hazards should the sludge be applied as a soil ameliorant.